Repositorio UFRO · Omeka S

Perfil de Metilación de Genes Supresores de Tumores APAF1, ASSP1, p73 y FHIT en Pacientes con Leucemia Linfoblástica Aguda Infantil

Primer Autor	Roa S, Juan Carlos
Co-autores	Melo A, Angelica Artigas A, Carmen Gloria Munoz N, Sergio Brebi M, Priscilla Hoffstetter G, Rene
Título	Perfil de Metilación de Genes Supresores de Tumores APAF1, ASSP1, p73 y FHIT en Pacientes con Leucemia Linfoblástica Aguda Infantil
Alternative Title	Detection of Hypermethylation in Tumor Suppressor Genes APAF1, ASSP1, p73 and FHIT in Childhood Acute Lymphoblastic Leukemia
Editorial	SOC CHILENA ANATOMIA
Revista	INTERNATIONAL JOURNAL OF MORPHOLOGY
Lenguaje	es
Resumen	Acute lymphoblastic leukemia (ALL) is the most common malignancy in children. It is manifested by a loss of differentiation of lymphoid progenitors, producing an increase of immature cells. Hypermethylation in promoter region of tumor suppressor genes (GST) may produce a gene silencing that provides a leukemic cell a proliferative advantage or prevent apoptosis. We studied the hypermethylation status of 4 GST involved in apoptosis: APAF1, ASPP1, p73 and FHIT and its association with survival of patients <15 years diagnosed with ALL. We analyzed 38 samples of bone marrow by DNA bisulfite modification and chain reaction methylation-specific polymerase (MSP). The mean age at diagnosis was 10 months to 13.8 years. Overall survival was 69% at 5 years. 81.5% of patients had at least one hypermethylated gene. The frequency observed was: APAF1 68.4%, 56.4% FHIT, p73 ASPP1 42% and 18.4%. The association between hypermethylation and group <5 years and >= 5 years was: Global p = 0.20, APAF1 p = 0.03, FHIT p = 0.51, p73 p = 0.51, ASPP1 p = 0.67. Survival curves were calculated by frequency of hypermethylation of each gene: APAF1 p = 0.05, p = 0.31 FHIT, p73 p = 0.98 and ASPP1 p = 0.82. The high frequency of hypermethylation obtained confirms enrollment of methylation in the promoter region of GST in the pathogenesis of ALL. APAF1 gene hypermethylation was very frequent and was significantly associated with survival in the study group, showing this gene as a predictor of poor prognosis in patients with ALL.
Tipo de Recurso	Artículo original
doi	10.4067/S0717-95022013000300033
Formato Recurso	pdf
Palabras Claves	Hypermethylation Childhood acute lymphoblastic leukemia Tumor suppressor genes p53 target genes Apoptosis Survival CPG ISLAND ABERRANT METHYLATION DNA METHYLATION PROMOTER CHILDREN INFANT ASPP1 TP53 Hipermetilación Leucemia linfoblástica aguda infantil Genes supresores de tumores Genes diana de p53 Apoptosis Supervivencia ISLA CPG METILACIÓN ABERRANTE METILACIÓN DEL ADN PROMOTOR NIÑOS LACTANTES ASPP1 TP53
Ubicación del archivo	http://dx.doi.org/10.4067/S0717-95022013000300033
Materias	Hypermethylation Childhood acute lymphoblastic leukemia Tumor suppressor genes p53 target genes Apoptosis Survival CPG ISLAND ABERRANT METHYLATION DNA METHYLATION PROMOTER CHILDREN INFANT ASPP1 TP53 Hipermetilación Leucemia linfoblástica aguda infantil Genes supresores de tumores Genes diana de p53 Apoptosis Supervivencia ISLA CPG METILACIÓN ABERRANTE METILACIÓN DEL ADN PROMOTOR NIÑOS LACTANTES ASPP1 TP53
Disciplinas de la OCDE	Oncología Genética Humana Biología Molecular
Título de la cita (Recomendado-único)	Perfil de Metilación de Genes Supresores de Tumores APAF1, ASSP1, p73 y FHIT en Pacientes con Leucemia Linfoblástica Aguda Infantil
Revista/Libro	INTERNATIONAL JOURNAL OF MORPHOLOGY
Tipo de ruta	dorada verde
Access Rights	acceso abierto
Derechos de acceso	acceso abierto
License	CC BY-NC
Condición de la licencia (Recomendado-repetible)	CC BY-NC
Idioma	es
Identificador del recurso (Mandatado-único)	Artículo original
Versión del recurso (Recomendado-único)	versión publicada
Editorial	SOC CHILENA ANATOMIA
ISSN	0717-9502
Página de inicio (Recomendado-único)	973
Página final (Recomendado-único)	979
Categoría WOS	Anatomía y morfología Anatomy & Morphology
Formato	pdf
Id de Web of Science	WOS:000327821700033