Repositorio UFRO · Omeka S

Criopreservación de Espermatozoides Caninos a - 80C

Primer Autor	Salinas, Paulo
Co-autores	Sanchez, Raul Risopatron, Jennie
Título	Criopreservación de Espermatozoides Caninos a - 80C
Alternative Title	Cryopreservation of Canine Spermatozoa at-80°C
Editorial	SOC CHILENA ANATOMIA
Revista	INTERNATIONAL JOURNAL OF MORPHOLOGY
Lenguaje	es
Resumen	The freezing and storage in liquid nitrogen (LN2) is the technique used most for the preservation of canine and other domestic species semen, for long periods of time. This study was designed to validate the use of deep freezer at -80 degrees C to freeze and store canine semen in straws. Three protocols were tested (n = 10): Control: sperm frozen in N2L and stored; Exp 1: sperm frozen and stored -80 degrees C, and Exp. 2: semen frozen at -80 degrees C and stored in N2L. Post-thawing was assessed by flow cytometry and the viability of plasma membrane integrity (SYBR-14/PI), mitochondrial membrane potential (Psi Delta m, JC-1), acrosome membrane integrity (PSA / FITC-PI), translocation of phosphatidylserine (Annexin -V-FITC/PI) and DNA fragmentation (TUNEL). No significant differences (P <0.05) between the Control, Exp. 2 and Exp. 1groups regarding: intact plasma membrane integrity (42.2 +/- 5.3, 35.57 +/- 10.3 and 40.76 +/- 12.1, respectively), Psi Delta m normal (54.7 +/- 20.6, 44.4 +/- 15.8 and 43.4 +/- 15.3, respectively), intact acrosomal membrane integrity (42.9 +/- 11, 1, 53.2 +/- 15.8 and 48.7 +/- 20.1, respectively) and fragmented DNA (0.87 +/- 0.41, 0.75 +/- 0.16, 0.79 +/- 0.27, respectively). However, the average motility of the post-thawing of Exp. 1 and Exp. 2 groups (37.0 +/- 4.4% 36.8 +/- 4.1% respectively) showed significant differences (P <0.05) than the Control group (55.9 +/- 8.7%). The results obtained in this study showed that the use of deep freezer at -80 degrees C for freezing and storing canine sperm has potential use in veterinary medicine as an alternative to the use of N2L.
Tipo de Recurso	Artículo original
doi	10.4067/S0717-95022013000100036
Formato Recurso	pdf
Palabras Claves	Canine Sperm freezing Ultrafreezer at-80 degrees C IN-VITRO SURVIVAL SEMEN QUALITY DOG SPERM FROZEN MOTILITY FRESH OF-152-DEGREES-C INSEMINATION APOPTOSIS Canino Congelación de esperma Ultracongelador a -80 grados centígrados Supervivencia in vitro calidad del semen perro esperma congelado motilidad fresco a 152 grados centígrados inseminación apoptosis
Ubicación del archivo	http://dx.doi.org/10.4067/S0717-95022013000100036
Materias	Canine Sperm freezing Ultrafreezer at-80 degrees C IN-VITRO SURVIVAL SEMEN QUALITY DOG SPERM FROZEN MOTILITY FRESH OF-152-DEGREES-C INSEMINATION APOPTOSIS Canino Congelación de esperma Ultracongelador a -80 grados centígrados Supervivencia in vitro calidad del semen perro esperma congelado motilidad fresco a 152 grados centígrados inseminación apoptosis
Disciplinas de la OCDE	Ciencias Veterinarias Ciencia Animal y lechería Biología Reproductiva
Título de la cita (Recomendado-único)	Criopreservación de Espermatozoides Caninos a - 80C
Revista/Libro	INTERNATIONAL JOURNAL OF MORPHOLOGY
Tipo de ruta	dorada verde
Access Rights	acceso abierto
Derechos de acceso	acceso abierto
License	CC BY-NC
Condición de la licencia (Recomendado-repetible)	CC BY-NC
Idioma	es
Identificador del recurso (Mandatado-único)	Artículo original
Versión del recurso (Recomendado-único)	versión publicada
Editorial	SOC CHILENA ANATOMIA
ISSN	0717-9502
Página de inicio (Recomendado-único)	217
Página final (Recomendado-único)	224
Categoría WOS	Anatomía y morfología Anatomy & Morphology
Formato	pdf
Id de Web of Science	WOS:000321868400036