Repositorio
Institucional

Roa, Juan C.

Garcia, Patricia

Leal, Pamela

Ili, Carmen

Brebi, Priscilla

Alvarez, Hector

Inhibition of connective tissue growth factor (CTGF/CCN2) in gallbladder cancer cells leads to decreased growth in vitro

WILEY

INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY

en

Gallbladder cancer (GBC) is an aggressive neoplasm associated with late diagnosis, unsatisfactory treatment and poor prognosis. Previous work showed that connective tissue growth factor (CTGF) expression is increased in this malignancy. This matricellular protein plays an important role in various cellular processes and its involvement in the tumorigenesis of several human cancers has been demonstrated. However, the precise function of CTGF expression in cancer cells is yet to be determined. The aim of this study was to evaluate the CTGF expression in gallbladder cancer cell lines, and its effect on cell viability, colony formation and in vitro cell migration. CTGF expression was evaluated in seven GBC cell lines by Western blot assay. Endogenous CTGF expression was downregulated by lentiviral shRNA directed against CTGF mRNA in G-415 cells, and the effects on cell viability, anchorage-independent growth and migration was assessed by comparing them to scrambled vector-transfected cells. Knockdown of CTGF resulted in significant reduction in cell viability, colony formation and anchorage-independent growth (P<0.05). An increased p27 expression was observed in G-415 cells with loss of CTGF function. Our results suggest that high expression of this protein in gallbladder cancer may confer a growth advantage for neoplastic cells.

Artículo original

We thank Dr. Anirban Maitra for providing the GB-d1, G-415 and SNU-308 gallbladder cancer cell lines and Dr. Juan Carcamo for HEK-293T and HeLa cells. This work was supported by FONDECYT Grant No1090171 (to JCR), DIUFRO project DI09-0082 (to PG) and Grant for support Doctoral Thesis CONICYT-24090177 (to PG).

Agradecemos al Dr. Anirban Maitra por proporcionar las líneas celulares de cáncer de vesícula biliar GB-d1, G-415 y SNU-308, y al Dr. Juan Carcamo por las células HEK-293T y HeLa. Este trabajo fue financiado por la beca FONDECYT n.° 1090171 (a JCR), el proyecto DIUFRO DI09-0082 (a PG) y la beca de apoyo a la tesis doctoral CONICYT-24090177 (a PG).

10.1111/iep.12023

pdf

connective tissue growth factor

gallbladder cancer cells

shRNA-mediated knock-down

FACTOR EXPRESSION

DOWN-REGULATION

GASTRIC-CANCER

TUMOR-GROWTH

CCN FAMILY

CTGF

INVASION

KINASE

PROLIFERATION

ANGIOGENESIS

factor de crecimiento del tejido conectivo

células de cáncer de vesícula biliar

silenciamiento génico mediado por shRNA

EXPRESIÓN DE FACTORES

REGULACIÓN NEGATIVA

CÁNCER GÁSTRICO

CRECIMIENTO TUMORIAL

FAMILIA CCN

CTGF

INVASIÓN

QUINASA

PROLIFERACIÓN

ANGIOGÉNESIS

connective tissue growth factor

gallbladder cancer cells

shRNA-mediated knock-down

FACTOR EXPRESSION

DOWN-REGULATION

GASTRIC-CANCER

TUMOR-GROWTH

CCN FAMILY

CTGF

INVASION

KINASE

PROLIFERATION

ANGIOGENESIS

factor de crecimiento del tejido conectivo

células de cáncer de vesícula biliar

silenciamiento génico mediado por shRNA

EXPRESIÓN DE FACTORES

REGULACIÓN NEGATIVA

CÁNCER GÁSTRICO

CRECIMIENTO TUMORIAL

FAMILIA CCN

CTGF

INVASIÓN

QUINASA

PROLIFERACIÓN

ANGIOGÉNESIS

Biología Celular

Biología Molecular

Oncología

Inhibition of connective tissue growth factor (CTGF/CCN2) in gallbladder cancer cells leads to decreased growth in vitro

INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY

hibrida

verde

metadata

metadata

PMID 23593935

en

Artículo original

versión publicada

ANID CONICYT FONDECYT 1090171

UFRO DI09-0082

ANID CONICYT Doctoral Thesis 24090177

ANID CONICYT FONDECYT 1090171

UFRO DI09-0082

ANID CONICYT Doctoral Thesis 24090177

WILEY

0959-9673

195

202

Patología

Pathology

pdf

WOS:000318989900004