Repositorio UFRO · Omeka S

Chemical and botanical characterization of Chilean propolis and biological activity on cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus

Primer Autor	Salazar, Luis A.
Co-autores	Barrientos, Leticia Herrera, Christian L. Montenegro, Gloria Ortega, Ximena Veloz, Jorge Alvear, Marysol Cuevas, Alejandro Saavedra, Nicolas
Título	Chemical and botanical characterization of Chilean propolis and biological activity on cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus
Editorial	SPRINGER
Revista	BRAZILIAN JOURNAL OF MICROBIOLOGY
Lenguaje	en
Resumen	Propolis is a non-toxic natural substance with multiple pharmacological properties including anticancer, antioxidant, fungicidal, antibacterial, antiviral, and anti-inflammatory among others. The aim of this study was to determine the chemical and botanical characterization of Chilean propolis samples and to evaluate their biological activity against the cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. Twenty propolis samples were obtained from beekeeping producers from the central and southern regions of Chile. The botanical profile was determined by palynological analysis. Total phenolic contents were determined using colorimetric assays. Reverse phase HPLC and HPLC-MS were used to determine the chemical composition. The minimum inhibitory concentration (MIC) was determined on S. mutans and S. sobrinus. All propolis samples were dominated by structures from native plant species. The characterization by HPLC/MS, evidenced the presence of quercetin, myricetin, kaempferol, rutine, pinocembrin, coumaric acid, caffeic acid and caffeic acid phenethyl ester, that have already been described in these propolis with conventional HPLC. Although all propolis samples inhibited the mutans streptococci growth, it was observed a wide spectrum of action (MIC 0.90 to 8.22 mu g mL(-1)). Given that results it becomes increasingly evident the need of standardization procedures, where we combine both the determination of botanical and the chemical characterization of the extracts. Research conducted to date, describes a promising effectiveness of propolis in the prevention of caries and other diseases of the oral cavity, making it necessary to develop studies to identify and understand the therapeutic targets or mechanisms of molecular action of the various compounds present on them.
Tipo de Recurso	Artículo original
Description	We gratefully acknowledge Rodrigo Pizarro and Gabriela Valdebenito for technical help in palynological and HPLC-MS analysis, respectively. This study was supported by grants from Direccion de Investigacion y Desarrollo (DIUFRO DI10-0031, DIUDRO DI09-1007, DIUFRO DI12-1002 and DIUFRO DI12-2013), Universidad de La Frontera, and CONICYT (FONDEF D05I-10021), Chile. We gratefully acknowledge Rodrigo Pizarro and Gabriela Valdebenito for technical help in palynological and HPLC-MS analysis, respectively. This study was supported by grants from Direccion de Investigacion y Desarrollo (DIUFRO DI10-0031, DIUDRO DI09-1007, DIUFRO DI12-1002 and DIUFRO DI12-2013), Universidad de La Frontera, and CONICYT (FONDEF D05I-10021), Chile.
doi	10.1590/S1517-83822013000200038
Formato Recurso	pdf
Palabras Claves	propolis botanical characterization chemical characterization Streptococcus mutans Streptococcus sobrinus VITRO ANTIMICROBIAL ACTIVITY APIS-MELLIFERA PROPOLIS CARIES DEVELOPMENT BEE PROPOLIS ANTIBACTERIAL GLUCOSYLTRANSFERASE CONSTITUENTS ANTIFUNGAL ADHERENCE ORIGINS propóleo caracterización botánica caracterización química Streptococcus mutans Streptococcus sobrinus ACTIVIDAD ANTIMICROBIANA IN VITRO PROPOLIS DE APIS MELLIFERA DESARROLLO DE CARIES PROPOLIS DE ABEJA ANTIBACTERIANO GLUCOSILTRANSFERASA COMPONENTES ANTIFÚNGICO ADHERENCIA ORÍGENES
Ubicación del archivo	http://dx.doi.org/10.1590/S1517-83822013000200038
Materias	propolis botanical characterization chemical characterization Streptococcus mutans Streptococcus sobrinus VITRO ANTIMICROBIAL ACTIVITY APIS-MELLIFERA PROPOLIS CARIES DEVELOPMENT BEE PROPOLIS ANTIBACTERIAL GLUCOSYLTRANSFERASE CONSTITUENTS ANTIFUNGAL ADHERENCE ORIGINS propóleo caracterización botánica caracterización química Streptococcus mutans Streptococcus sobrinus ACTIVIDAD ANTIMICROBIANA IN VITRO PROPOLIS DE APIS MELLIFERA DESARROLLO DE CARIES PROPOLIS DE ABEJA ANTIBACTERIANO GLUCOSILTRANSFERASA COMPONENTES ANTIFÚNGICO ADHERENCIA ORÍGENES
Disciplinas de la OCDE	Farmacología y Farmacia Odontología, Cirugía y Medicina Oral
Título de la cita (Recomendado-único)	Chemical and botanical characterization of Chilean propolis and biological activity on cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus
Revista/Libro	BRAZILIAN JOURNAL OF MICROBIOLOGY
Tipo de ruta	diamante verde
Access Rights	acceso abierto
Derechos de acceso	acceso abierto
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Identificador alternativo	PMID 24294257
Idioma	en
Identificador del recurso (Mandatado-único)	Artículo original
Versión del recurso (Recomendado-único)	versión publicada
Entidad financiadora	UFRO DI10-0031#UFRO DI09-1007#UFRO DI12-1002#UFRO DI12-2013#ANID CONICYT FONDEF D05I-10021
Referencia del Financiador (Mandatado si es aplicable-repetible)	UFRO DI10-0031 UFRO DI09-1007 UFRO DI12-1002 UFRO DI12-2013 ANID CONICYT FONDEF D05I-10021
Editorial	SPRINGER
ISSN	1517-8382
Página de inicio (Recomendado-único)	577
Página final (Recomendado-único)	585
Categoría WOS	Microbiología Microbiology
Formato	pdf
Id de Web of Science	WOS:000325780000037