Repositorio
Institucional

Arias, Maria E.

Ross, Pablo J.

Felmer, Ricardo N.

Culture medium composition affects the gene expression pattern and in vitro development potential of bovine somatic cell nuclear transfer (SCNT) embryos

SOC BIOLGIA CHILE

BIOLOGICAL RESEARCH

en

Different culture systems have been studied that support development of somatic cell nuclear transfer (SCNT) embryos up to the blastocyst stage. However, the use of sequential and two-step culture systems has been less studied. The objective of the present study was to examine the developmental potential and quality of bovine SCNT embryos cultured in different two-step culture media based on KSOM, SOF and the macromolecules FBS and BSA (K-K/FBS, K-S/BSA and K-K/BSA, respectively). No differences were observed in the cleavage rate for any of the culture systems. However, there was a significant difference (P<0.01) in the rate of blastocyst development, with the K-K/FBS culture system yielding a higher rate of blastocysts (28%) compared to other treatments (18 and 15%, for K-S/BSA and K-K/BSA, respectively). Although quality of embryos, as assessed by the total number of cells, was not different, the apoptosis index was significantly affected in the sequential culture system (K-S/BSA). Gene expression analysis showed alterations of DNMT1, IGF2, LIF, and PRDX6 genes in embryos cultured in K-S/FBS and of SOD2 in embryos cultured in K-K/BSA. In conclusion, we demonstrated that culture medium may affect not only the developmental potential of SCNT embryos but also, more importantly, the gene expression pattern and apoptotic index, presenting the possibility to manipulate the culture medium composition to modulate global gene expression and improve the overall efficiency of this technique.

Artículo original

The provision of ovaries by our local slaughterhouse (Frigorifico Temuco, Temuco, Chile) and funding support from CONICYT-Chile, grant FONDECYT 1080216 are gratefully acknowledged.

Agradecemos la donación de ovarios por parte de nuestro matadero local (Frigorifico Temuco, Temuco, Chile) y el apoyo financiero de CONICYT-Chile, subvención FONDECYT 1080216.

10.4067/S0716-97602013000400016

pdf

Cattle

gene expression

KSOM

nuclear transfer embryos

SOF

MESSENGER-RNA EXPRESSION

OVIDUCT FLUID MEDIUM

FETAL CALF SERUM

PROTEIN SUPPLEMENTATION

PREIMPLANTATION EMBRYOS

DNA METHYLTRANSFERASE

2-STEP CULTURE

AMINO-ACIDS

CALVES

BLASTOCYST

Ganado vacuno

expresión génica

KSOM

embriones por transferencia nuclear

SOF

EXPRESIÓN DE ARN MENSAJERO

MEDIO DE FLUIDO DE OVIDUCTO

SUERO FETAL DE TERNERO

SUPLEMENTACIÓN PROTEICA

EMBRIONES PREIMPLANTADOS

ADN METILTRANSFERASA

CULTIVO EN DOS ETAPAS

AMINOÁCIDOS

TERNEROS

BLASTOCISTO

Cattle

gene expression

KSOM

nuclear transfer embryos

SOF

MESSENGER-RNA EXPRESSION

OVIDUCT FLUID MEDIUM

FETAL CALF SERUM

PROTEIN SUPPLEMENTATION

PREIMPLANTATION EMBRYOS

DNA METHYLTRANSFERASE

2-STEP CULTURE

AMINO-ACIDS

CALVES

BLASTOCYST

Ganado vacuno

expresión génica

KSOM

embriones por transferencia nuclear

SOF

EXPRESIÓN DE ARN MENSAJERO

MEDIO DE FLUIDO DE OVIDUCTO

SUERO FETAL DE TERNERO

SUPLEMENTACIÓN PROTEICA

EMBRIONES PREIMPLANTADOS

ADN METILTRANSFERASA

CULTIVO EN DOS ETAPAS

AMINOÁCIDOS

TERNEROS

BLASTOCISTO

Tecnologías que involucran la manipulación de Células , Tejidos, órganos o el organismo completo (Reproducción Asistida)

Biología del Desarrollo

Biología Reproductiva

Culture medium composition affects the gene expression pattern and in vitro development potential of bovine somatic cell nuclear transfer (SCNT) embryos

BIOLOGICAL RESEARCH

dorada

verde

acceso abierto

acceso abierto

CC BY NC 4.0

en

Artículo original

versión publicada

ANID CONICYT FONDECYT 1080216

ANID CONICYT FONDECYT 1080216

SOC BIOLGIA CHILE

0716-9760

453

462

Biología

Biology

pdf

WOS:000330202500016