Repositorio
Institucional

Crespo Hirata, Rosario Dominguez

de Oliveira, Raquel

Cerda, Alvaro

Genvigir, Fabiana Dalla Vecchia

Ferraz Sampaio, Marcelo

Armaganijan, Dikran

Silveira Bernik, Marcia Martins

Dorea, Egidio Lima

Hirata, Mario Hiroyuki

Hinuy, Hamilton Massayuki

Leptin receptor gene polymorphisms are associated with adiposity and metabolic alterations in Brazilian individuals

SBEM-SOC BRASIL ENDOCRINOLOGIA & METABOLOGIA

ARQUIVOS BRASILEIROS DE ENDOCRINOLOGIA E METABOLOGIA

en

Objective: The aim of the study was to investigate whether adiposity and metabolic markers, such as leptin, glucose, and lipids, are influenced by leptin (LEP) and leptin receptor (LEPR) gene polymorphisms in a sample of our population. Subjects and methods: A group of 326 individuals of Caucasian-European descent, aged 30 to 80 years, 87 men and 239 women, 148 obese and 178 non-obese, was randomly selected at two clinical hospitals in the city of Sao Paulo, Brazil. All individuals declared their ethnic group as white during the initial interview. Anthropometric measurements, body mass index (BMI), and fat mass were evaluated. Blood samples were drawn for DNA extraction and measurements of leptin, soluble leptin receptor, glucose, and lipids. LEP -2548G> A and LEPR Lys109Arg (c.326A>G), Gln233Arg (c.668A>G) and Lys656Asn (c.1968G>C) polymorphisms were detected by PCR-RFLP. Results: Increased leptin and serum lipids, and LEPR Arg223Arg (GG genotype) were associated with higher risk for obesity (p < 0.05), while reduced risk was found in LEPR Arg109Arg (GG genotype) carriers (OR: 0.38, 95% CI: 0.19-0.77, p = 0.007). Multiple linear regression analysis showed a relationship between LEPR 223Arg, increased waist circumference, and leptinemia (p < 0.05), while LEPR 109Arg was associated with high total cholesterol and triglycerides (p < 0.05). LEPR haplotype 3 (AGG: 109Lys/233Arg/656Lys) carriers have increased risk for obesity (OR: 2.56, 95% CI: 1.19-5.49, p = 0.017). Moreover, this haplotype was associated with increased BMI, waist circumference, and leptinemia (p < 0.05). Conclusions: LEPR polymorphisms are associated with obesity, hyperleptinemia, and atherogenic lipid profile, suggesting their potential role for leptin resistance and cardiovascular risk. Moreover, LEPR haplotype 3 confers susceptibility to adiposity and hyperleptinemia in our population.

Artículo original

this study was supported by a grant from Fapesp (Protocol

2009/10069-2). We would like to thank Tamiris I. Moraes and Rebecca Kiyokawa for their technical support. R. Oliveira and F. D. V. Genvigir were recipients of fellowship from Fapesp, Brazil. A. Cerda was recipient of a fellowship from CONICYT, Chile. M. H. Hirata and R. D. C. Hirata are recipients of fellowship from CNPq, Brazil.

Este estudio fue financiado por una subvención de Fapesp (Protocolo n.° 2009/10069-2). Agradecemos a Tamiris I. Moraes y Rebecca Kiyokawa su apoyo técnico. R. Oliveira y F. D. V. Genvigir recibieron una beca de Fapesp, Brasil. A. Cerda recibió una beca de CONICYT, Chile. M. H. Hirata y R. D. C. Hirata recibieron una beca del CNPq, Brasil.

10.1590/S0004-27302013000900002

pdf

Leptin

leptin receptor

gene polymorphisms

adiposity

lipids

BODY-MASS INDEX

COMMON VARIANTS

HUMAN OBESITY

LEPR

RESISTANCE

PATHWAYS

DISEASE

TRAITS

FAMILY

Leptina

receptor de leptina

polimorfismos genéticos

adiposidad

lípidos

ÍNDICE DE MASA CORPORAL

VARIANTES COMUNES

OBESIDAD HUMANA

LEPR

RESISTENCIA

VÍAS

ENFERMEDAD

RASGOS

FAMILIA

Leptin

leptin receptor

gene polymorphisms

adiposity

lipids

BODY-MASS INDEX

COMMON VARIANTS

HUMAN OBESITY

LEPR

RESISTANCE

PATHWAYS

DISEASE

TRAITS

FAMILY

Leptina

receptor de leptina

polimorfismos genéticos

adiposidad

lípidos

ÍNDICE DE MASA CORPORAL

VARIANTES COMUNES

OBESIDAD HUMANA

LEPR

RESISTENCIA

VÍAS

ENFERMEDAD

RASGOS

FAMILIA

Endocrinología y Metabolismo (Incluye Diabetes, Hormonas)

Genética Humana

Leptin receptor gene polymorphisms are associated with adiposity and metabolic alterations in Brazilian individuals

ARQUIVOS BRASILEIROS DE ENDOCRINOLOGIA E METABOLOGIA

dorada

verde

acceso abierto

acceso abierto

CC BY NC 4.0

PMID 24402012

en

Artículo original

versión publicada

ANID CONICYT #CNPq, Brazil#FAPESP 2009/10069-2

ANID CONICYT

CNPq, Brazil

FAPESP 2009/10069-2

SBEM-SOC BRASIL ENDOCRINOLOGIA & METABOLOGIA

0004-2730

677

684

Endocrinología y metabolismo

Endocrinology & Metabolism

pdf

WOS:000329452700002