Repositorio
Institucional

Oliveira, R. P. S.

Figueroa, E.#Farias, J. G.#Lee-Estevez, M.#Valdebenito, I#Risopatron, J.#Magnotti, C.#Romero, J.#Watanabe, I

Sperm cryopreservation with supplementation of α-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)

ELSEVIER SCIENCE BV

AQUACULTURE

en

The use of alpha-tocopherol and ascorbic acid in freezing medium prevents lipoperoxidation and damage by ROS (reactive oxygen species), however, the effects of these antioxidants in salmonid spermatozoa have not been studied. In this work the protective effects of antioxidants on the enzymatic activity and fertilization capacity of frozen Atlantic salmon spermatozoa were studied. Spermatozoa were frozen in Cortland (R) medium supplemented with 1.3 M DMSO, 0.3 M glucose, 2% BSA and different concentrations of alpha-tocopherol and ascorbic acid, spermatozoa frozen without antioxidants were used as control and fresh sperm. Samples loaded in 0.5 ml plastic straws were frozen at 68 degrees C/min (Freeze Control (R)). Thawing was carried out at 35 degrees C and post-thawing lipid peroxidation (LPO) was evaluated along with Glutathione (GSH/GSSG) levels, and Glutathione peroxidase (GPx) and Catalase (CAT) activity. Additionally, percentage of motility, mitochondrial membrane potential (ATM), and fertilization rate were assessed. Results showed significantly lower LPO values (expressed as malondialdehyde concentration) in spermatozoa cryopreserved with alpha-tocopherol (0.1 mM and 0.5 mM) and with combinations of alpha-tocopherol/ascorbic acid (0.1 mM/1.0 mM and 0.5 mM/10 mM ratios) (p < .05). Moreover, spermatozoa frozen with alpha-tocopherol/ascorbic acid also exhibited significantly higher GPx and CAT activity as well as GSH/GSSG ratio compared to controls (p < .05). The production of O-2(i)- also decreased with the combined treatment, however, no statistically significant difference was found compared to controls. Consistently with the improvement in antioxidant defences, the percentage of motility and Delta Psi M were highest and significantly different in spermatozoa cryopreserved with alpha-tocopherol/ascorbic acid 0.1 mM/1.0 mM compared to controls, supporting the significantly higher fertilization rate exhibited by spermatozoa under the same treatment.

Artículo original

This work was supported by the Fund for the Promotion of Scientific and Technological Development of CONICYT, FONDECYT/POSTDOCTORAL (No 3180765) and FONDECYT/REGULAR (No 1180387) Chile, and the companies AquaGen Chile S.A. and Hendrix Genetics S.A. which provided the gametes used in the biotests.

Este trabajo fue apoyado por el Fondo de Promoción del Desarrollo Científico y Tecnológico de CONICYT, FONDECYT/POSTDOCTORAL (N° 3180765) y FONDECYT/REGULAR (N° 1180387) Chile, y las empresas AquaGen Chile S.A. y Hendrix Genetics S.A. que proporcionaron los gametos utilizados en los bioensayos.

10.1016/j.aquaculture.2018.04.046

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Salmo salar# Spermatozoa# Cryopreservation# Antioxidant defences# alpha-tocopherol# Ascorbic acid

http://dx.doi.org/10.1016/j.aquaculture.2018.04.046

Fisheries# Marine & Freshwater Biology

salmo salar# Espermatozoide# criopreservación# Defensas antioxidantes# alfa-tocoferol# ácido ascórbico

Pesca

Biología Reproductiva

Bioquímica

WOS:000432906800001

Sperm cryopreservation with supplementation of α-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (<i>Salmo salar</i>)

Artículo original

version publicada

ELSEVIER SCIENCE BV

AQUACULTURE

Pesca# Biología marina y de agua dulce

0044-8486

en

ANID CONICYT FONDECYT/POSTDOCTORAL 3180765#ANID FONDECYT REGULAR 1180387

ANID CONICYT FONDECYT/POSTDOCTORAL 3180765

ANID FONDECYT/REGULAR 1180387

AquaGen Chile S.A.

Hendrix Genetics S.A.

This work was supported by the Fund for the Promotion of Scientific and Technological Development of CONICYT, FONDECYT/POSTDOCTORAL (No 3180765) and FONDECYT/REGULAR (No 1180387) Chile, and the companies AquaGen Chile S.A. and Hendrix Genetics S.A. which provided the gametes used in the biotests.

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hibrida#verde

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