Repositorio
Institucional

Crainey, James Lee

Marin, Michel Abanto#Ribeiro da Silva, Tullio Romao#de Medeiros, Jansen Fernandes#Costa Pessoa, Felipe Arley#Santos, Yago Vinicius#Paulo Vicente, Ana Carolina#Bessa Luz, Sergio Luiz

Mansonella ozzardi mitogenome and pseudogene characterisation provides new perspectives on filarial parasite systematics and CO-1 barcoding

NATURE PORTFOLIO

SCIENTIFIC REPORTS

en

"Despite the broad distribution of M. ozzardi in Latin America and the Caribbean, there is still very little DNA sequence data available to study this neglected parasite's epidemiology. Mitochondrial DNA (mtDNA) sequences, especially the cytochrome oxidase (CO1) gene's barcoding region, have been targeted successfully for filarial diagnostics and for epidemiological, ecological and evolutionary studies. MtDNA-based studies can, however, be compromised by unrecognised mitochondrial pseudogenes, such as Numts. Here, we have used shot-gun Illumina-HiSeq sequencing to recover the first complete Mansonella genus mitogenome and to identify several mitochondrial-origin pseudogenes. Mitogenome phylogenetic analysis placed M. ozzardi in the Onchocercidae ""ONC5"" clade and suggested that Mansonella parasites are more closely related to Wuchereria and Brugia genera parasites than they are to Loa genus parasites. DNA sequence alignments, BLAST searches and conceptual translations have been used to compliment phylogenetic analysis showing that M. ozzardi from the Amazon and Caribbean regions are near-identical and that previously reported Peruvian M. ozzardi CO1 reference sequences are probably of pseudogene origin. In addition to adding a much-needed resource to the Mansonella genus's molecular tool-kit and providing evidence that some M. ozzardi CO1 sequence deposits are pseudogenes, our results suggest that all Neotropical M. ozzardi parasites are closely related."

Artículo original

The authors would like to gratefully acknowledge financial support from Fundacao de Amparo a Pesquisa do Estado do Amazonas (FAPEAM), which provided financial support for this work with the grant award: 062.02005/2014. We would also like to gratefully acknowledge financial support from the Fundacao de Amparo a Pesquisa do Estado do Amazonas (FAPEAM) and Programa Pesquisa Sistema Unico de Saude (PPSUS) which provided additional financial support with grant award: 062.00647/2014.

Los autores desean agradecer el apoyo financiero de la Fundacao de Amparo a Pesquisa do Estado do Amazonas (FAPEAM), que brindó apoyo financiero para este trabajo con la subvención: 062.02005/2014. También nos gustaría agradecer el apoyo financiero de la Fundacao de Amparo a Pesquisa do Estado do Amazonas (FAPEAM) y el Programa Pesquisa Sistema Unico de Saude (PPSUS) que brindaron apoyo financiero adicional con la subvención: 062.00647/2014.

10.1038/s41598-018-24382-3

pdf

http://dx.doi.org/10.1038/s41598-018-24382-3

Parasitology

Parasitología

Biología Molecular

Genética y Herencia

WOS:000430280600010

<i>Mansonella ozzardi</i> mitogenome and pseudogene characterisation provides new perspectives on filarial parasite systematics and CO-1 barcoding

Artículo original

version publicada

NATURE PORTFOLIO

SCIENTIFIC REPORTS

Parasitología

2045-2322

en

FAPEAM 062.02005/2014#FAPEAM 062.00647/2014

FAPEAM 062.02005/2014

PPSUS 062.00647/2014

The authors would like to gratefully acknowledge financial support from Fundacao de Amparo a Pesquisa do Estado do Amazonas (FAPEAM), which provided financial support for this work with the grant award: 062.02005/2014. We would also like to gratefully acknowledge financial support from the Fundacao de Amparo a Pesquisa do Estado do Amazonas (FAPEAM) and Programa Pesquisa Sistema Unico de Saude (PPSUS) which provided additional financial support with grant award: 062.00647/2014.

pdf

dorada#verde

acceso abierto

acceso abierto

CC BY

1309

1315