Repositorio
Institucional

Brebi, Priscilla

Buchegger, Kurt#Viscarra, Tamara#Andana, Alejandra#Ili, Carmen#Lopez, Jaime#Zanella, Louise#Ines Carmona-Lopez, Maria#Jose Fernandez, Juan#Cartas Espinel, Irene#Sanchez, Raul#Carlos Roa, Juan

Detection and genotyping of human papillomavirus virus (HPV): a comparative analysis of clinical performance in cervical and urine samples in Chilean women

E-CENTURY PUBLISHING CORP

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY

en

Human papillomavirus (HPV) is the most common sexually transmitted infectious agent and is the main cause of cervical cancer (CC). In Chile, CC is the second leading cause of death by cancer in women aged 20-44 years, four times higher than in developed countries. Currently, the detection of HPV infection using a cervical brush is recommended, however, this is an invasive procedure that many women try to avoid. The aim of this study was to evaluate the clinical performance of a self-collected, urine-based HPV detection method using conventional PCR followed by a reverse line blot. A PCR-based HPV genotyping was performed on 190 paired cervical and urine samples from gynecological exams at public health centers in the Araucania Region, Chile. HPV DNA detection and genotyping were performed by PCR and reverse line blot assay. Carcinogenic HPV types were present in 64.7% and 65.8% of the cervical and urine samples, the infection rates of HPV16 were 34.7% and 33.2%, respectively. The overall percent agreement between carcinogenic HPV detection in cervical and urine samples was 73.7%, with a moderate concordance rate of carcinogenic HPV detection (kappa = 0.42). Clinical sensitivities for cervical and urine-based sampling methods to diagnose cervical intraepithelial neoplasia 2/3 (CIN2/3) by histology were 93.4% and 90.2%, respectively. These results suggest that both cervical brush and urine-based sampling show a good clinical performance in the detection of HPV infection. The urine-based sampling method represents a valuable alternative with a great impact on public health, allowing increased cervical cancer screening coverage among women who do not undergo pelvic examinations.

Artículo original

We would like to thank everyone from the participating healthcare institutions who collaborated in this study. This work was supported by the National Fund for Scientific and Technological Development of Chile (FONDECYT) [11150802 to P.B., 11150622 to C.G.I., 3180550 to K.B.], the Corporation for Production Development (CORFO) [12IDL2-18157, 09CN-14-5960 to CEGIN], Direccion de Investigacion, Universidad de La Frontera (DIUFRO) [DI14-0072 to A.A and J.L., DI17-0079 to K.B.], the National Commission for Scientific and Technological Research - the Fund for Research Center in Priority Areas (CONICYT-FONDAP) [15130011 to JCR] and The Millennium Institute on Immunology and Immunotherapy [No P09-016-F to JCR]. The authors alone are responsible for the content and writing of this article.

Nos gustaría agradecer a todos los integrantes de las instituciones de salud participantes que colaboraron en este estudio. Este trabajo fue financiado por el Fondo Nacional de Desarrollo Científico y Tecnológico de Chile (FONDECYT) [11150802 a P.B., 11150622 a C.G.I., 3180550 a K.B.], la Corporación de Fomento de la Producción (CORFO) [12IDL2-18157, 09CN-14-5960 a CEGIN], la Dirección de Investigación de la Universidad de La Frontera (DIUFRO) [DI14-0072 a A.A y J.L., DI17-0079 a K.B.], la Comisión Nacional de Investigación Científica y Tecnológica - Fondo para Centros de Investigación en Áreas Prioritarias (CONICYT-FONDAP) [15130011 a JCR] y el Instituto Milenio de Inmunología e Inmunoterapia [No P09-016-F a JCR]. Los autores son los únicos responsables del contenido y la redacción de este artículo.

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Human papillomavirus# cervical intraepithelial neoplasia# cervical cancer# urine-based sampling method

https://e-century.us/files/ijcep/11/11/ijcep0079703.pdf

Oncology# Pathology

Virus del papiloma humano# neoplasia intraepitelial cervical# cáncer de cuello uterino# método de muestreo basado en orina

Biotecnología Relacionada con la Salud

Salud Pública y Ambiental

Virología

WOS:000451855800030

Detection and genotyping of human papillomavirus virus (HPV): a comparative analysis of clinical performance in cervical and urine samples in Chilean women

PMC6963012

Artículo original

version publicada

E-CENTURY PUBLISHING CORP

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY

Oncología# Patología

1936-2625

en

ANID FONDECYT 11150802#ANID FONDECYT 11150622#ANID FONDECYT 3180550#CORFO 12IDL2-18157#CORFO 09CN-14-5960#UFRO DI14-0072#UFRO DI17-0079#ANID CONICYT-FONDAP 15130011#MIII P09-016-F

ANID FONDECYT 11150802

ANID FONDECYT 11150622

ANID FONDECYT 3180550]

CORFO 12IDL2-18157

CORFO 09CN-14-5960]

UFRO DI14-0072

UFRO DI17-0079

ANID CONICYT FONDAP) 15130011

Instituto Milenio de Immunología e Immnuoterapia P09-016-F

We would like to thank everyone from the participating healthcare institutions who collaborated in this study. This work was supported by the National Fund for Scientific and Technological Development of Chile (FONDECYT) [11150802 to P.B., 11150622 to C.G.I., 3180550 to K.B.], the Corporation for Production Development (CORFO) [12IDL2-18157, 09CN-14-5960 to CEGIN], Direccion de Investigacion, Universidad de La Frontera (DIUFRO) [DI14-0072 to A.A and J.L., DI17-0079 to K.B.], the National Commission for Scientific and Technological Research - the Fund for Research Center in Priority Areas (CONICYT-FONDAP) [15130011 to JCR] and The Millennium Institute on Immunology and Immunotherapy [No P09-016-F to JCR]. The authors alone are responsible for the content and writing of this article.

pdf

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