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Guerrero-Preston, Rafael White, James Robert Godoy-Vitorino, Filipa Rodriguez-Hilario, Arnold Navarro, Kelvin Gonzalez, Herminio Michailidi, Christina Jedlicka, Anne Canapp, Sierra Bondy, Jessica Dziedzic, Amanda Mora-Lagos, Barbara Rivera-Alvarez, Gustavo Ili-Gangas, Carmen Brebi-Mieville, Priscilla Westra, William Koch, Wayne Kang, Hyunseok Marchionni, Luigi Kim, Young Sidransky, David

High-resolution microbiome profiling uncovers Fusobacterium nucleatum, Lactobacillus gasseri/johnsonii, and Lactobacillus vaginalis associated to oral and oropharyngeal cancer in saliva from HPV positive and HPV negative patients treated with surgery and chemo-radiation

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ONCOTARGET

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Microbiome studies show altered microbiota in head and neck squamous cell carcinoma (HNSCC), both in terms of taxonomic composition and metabolic capacity. These studies utilized a traditional bioinformatics methodology, which allows for accurate taxonomic assignment down to the genus level, but cannot accurately resolve species level membership. We applied Resphera Insight, a high-resolution methodology for 16S rRNA taxonomic assignment that is able to provide species-level context in its assignments of 16S rRNA next generation sequencing (NGS) data. Resphera Insight applied to saliva samples from HNSCC patients and healthy controls led to the discovery that a subset of HNSCC saliva samples is significantly enriched with commensal species from the vaginal flora, including Lactobacillus gasseri/johnsonii (710x higher in saliva) and Lactobacillus vaginalis (52x higher in saliva). These species were not observed in normal saliva from Johns Hopkins patients, nor in 16S rRNA NGS saliva samples from the Human Microbiome Project (HMP). Interestingly, both species were only observed in saliva from Human Papilloma Virus (HPV) positive and HPV negative oropharyngeal cancer patients. We confirmed the representation of both species in HMP data obtained from mid-vagina (n=128) and vaginal introitus (n=121) samples. Resphera Insight also led to the discovery that Fusobacterium nucleatum, an oral cavity flora commensal bacterium linked to colon cancer, is enriched (600x higher) in saliva from a subset of HNSCC patients with advanced tumors stages. Together, these high-resolution analyses on 583 samples suggest a possible role for bacterial species in the therapeutic outcome of HPV positive and HPV negative HNSCC patients.

Artículo original

This research was supported in part by National Cancer Institute grants U01CA84986, K01CA164092 and CA121113, National Institute of Dental and Craniofacial Research grants P50DE019032 Head and Neck Cancer SPORE, RC2 DE20957 and NIGMS/INBRE award P20 GM103475-15.

Esta investigación fue financiada en parte por las subvenciones U01CA84986, K01CA164092 y CA121113 del Instituto Nacional del Cáncer, las subvenciones P50DE019032 SPORE, RC2 DE20957 del Instituto Nacional de Investigación Dental y Craneofacial y el premio NIGMS/INBRE P20 GM103475-15.

10.18632/oncotarget.20677

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microbiota# high-resolution microbiome profiling# oral cancer# oropharyngeal cancer# human microbiome project

http://dx.doi.org/10.18632/oncotarget.20677

Oncology# Cell Biology

microbiota# perfiles de microbioma de alta resolución# cáncer bucal# cáncer de orofaringe# proyecto de microbioma humano

Microbiología

Oncología

Biotecnología Relacionada con la Salud

WOS:000419567000021

High-resolution microbiome profiling uncovers <i>Fusobacterium nucleatum</i>, <i>Lactobacillus gasseri</i>/<i>johnsonii</i>, and <i>Lactobacillus vaginalis</i> associated to oral and oropharyngeal cancer in saliva from HPV positive and HPV negative patients treated with surgery and chemo-radiation

Artículo original

version publicada

IMPACT JOURNALS LLC

ONCOTARGET

Oncología# Biología celular

en

National Cancer Institute U01CA84986#National Cancer Institute K01CA164092#National Cancer Institute CA121113#National Institute of Dental and Craniofacial Research grants P50DE019032#Head and Neck Cancer SPORE, RC2 DE20957#NIGMS/INBRE P20 GM103475-15

NCI U01CA84986

NCI K01CA164092

NCI CA121113

NIH NIDCR P50DE019032

NIGMS/INBRE P20 GM103475-15

This research was supported in part by National Cancer Institute grants U01CA84986, K01CA164092 and CA121113, National Institute of Dental and Craniofacial Research grants P50DE019032 Head and Neck Cancer SPORE, RC2 DE20957 and NIGMS/INBRE award P20 GM103475-15.

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