High-resolution microbiome profiling uncovers Fusobacterium nucleatum, Lactobacillus gasseri/johnsonii, and Lactobacillus vaginalis associated to oral and oropharyngeal cancer in saliva from HPV positive and HPV negative patients treated with surgery and chemo-radiation

Primer Autor
Guerrero-Preston, Rafael White, James Robert Godoy-Vitorino, Filipa Rodriguez-Hilario, Arnold Navarro, Kelvin Gonzalez, Herminio Michailidi, Christina Jedlicka, Anne Canapp, Sierra Bondy, Jessica Dziedzic, Amanda Mora-Lagos, Barbara Rivera-Alvarez, Gustavo Ili-Gangas, Carmen Brebi-Mieville, Priscilla Westra, William Koch, Wayne Kang, Hyunseok Marchionni, Luigi Kim, Young Sidransky, David
Título
High-resolution microbiome profiling uncovers Fusobacterium nucleatum, Lactobacillus gasseri/johnsonii, and Lactobacillus vaginalis associated to oral and oropharyngeal cancer in saliva from HPV positive and HPV negative patients treated with surgery and chemo-radiation
Editorial
IMPACT JOURNALS LLC
Revista
ONCOTARGET
Lenguaje
en
Resumen
Microbiome studies show altered microbiota in head and neck squamous cell carcinoma (HNSCC), both in terms of taxonomic composition and metabolic capacity. These studies utilized a traditional bioinformatics methodology, which allows for accurate taxonomic assignment down to the genus level, but cannot accurately resolve species level membership. We applied Resphera Insight, a high-resolution methodology for 16S rRNA taxonomic assignment that is able to provide species-level context in its assignments of 16S rRNA next generation sequencing (NGS) data. Resphera Insight applied to saliva samples from HNSCC patients and healthy controls led to the discovery that a subset of HNSCC saliva samples is significantly enriched with commensal species from the vaginal flora, including Lactobacillus gasseri/johnsonii (710x higher in saliva) and Lactobacillus vaginalis (52x higher in saliva). These species were not observed in normal saliva from Johns Hopkins patients, nor in 16S rRNA NGS saliva samples from the Human Microbiome Project (HMP). Interestingly, both species were only observed in saliva from Human Papilloma Virus (HPV) positive and HPV negative oropharyngeal cancer patients. We confirmed the representation of both species in HMP data obtained from mid-vagina (n=128) and vaginal introitus (n=121) samples. Resphera Insight also led to the discovery that Fusobacterium nucleatum, an oral cavity flora commensal bacterium linked to colon cancer, is enriched (600x higher) in saliva from a subset of HNSCC patients with advanced tumors stages. Together, these high-resolution analyses on 583 samples suggest a possible role for bacterial species in the therapeutic outcome of HPV positive and HPV negative HNSCC patients.
Tipo de Recurso
Artículo original
Description
This research was supported in part by National Cancer Institute grants U01CA84986, K01CA164092 and CA121113, National Institute of Dental and Craniofacial Research grants P50DE019032 Head and Neck Cancer SPORE, RC2 DE20957 and NIGMS/INBRE award P20 GM103475-15.
Esta investigación fue financiada en parte por las subvenciones U01CA84986, K01CA164092 y CA121113 del Instituto Nacional del Cáncer, las subvenciones P50DE019032 SPORE, RC2 DE20957 del Instituto Nacional de Investigación Dental y Craneofacial y el premio NIGMS/INBRE P20 GM103475-15.
doi
10.18632/oncotarget.20677
Formato Recurso
pdf
Palabras Claves
microbiota# high-resolution microbiome profiling# oral cancer# oropharyngeal cancer# human microbiome project
Ubicación del archivo
http://dx.doi.org/10.18632/oncotarget.20677
Categoría OCDE
Oncology# Cell Biology
Materias
microbiota# perfiles de microbioma de alta resolución# cáncer bucal# cáncer de orofaringe# proyecto de microbioma humano
Disciplinas de la OCDE
Microbiología
Oncología
Biotecnología Relacionada con la Salud
Id de Web of Science
WOS:000419567000021
Título de la cita (Recomendado-único)
High-resolution microbiome profiling uncovers <i>Fusobacterium nucleatum</i>, <i>Lactobacillus gasseri</i>/<i>johnsonii</i>, and <i>Lactobacillus vaginalis</i> associated to oral and oropharyngeal cancer in saliva from HPV positive and HPV negative patients treated with surgery and chemo-radiation
Identificador del recurso (Mandatado-único)
Artículo original
Versión del recurso (Recomendado-único)
version publicada
Editorial
IMPACT JOURNALS LLC
Revista/Libro
ONCOTARGET
Categoría WOS
Oncología# Biología celular
Idioma
en
Referencia del Financiador (Mandatado si es aplicable-repetible)
National Cancer Institute U01CA84986#National Cancer Institute K01CA164092#National Cancer Institute CA121113#National Institute of Dental and Craniofacial Research grants P50DE019032#Head and Neck Cancer SPORE, RC2 DE20957#NIGMS/INBRE P20 GM103475-15
NCI U01CA84986
NCI K01CA164092
NCI CA121113
NIH NIDCR P50DE019032
NIGMS/INBRE P20 GM103475-15
Descripción
This research was supported in part by National Cancer Institute grants U01CA84986, K01CA164092 and CA121113, National Institute of Dental and Craniofacial Research grants P50DE019032 Head and Neck Cancer SPORE, RC2 DE20957 and NIGMS/INBRE award P20 GM103475-15.
Formato
pdf
Tipo de ruta
dorada#verde
Access Rights
acceso abierto
Derechos de acceso
acceso abierto
License
CC BY
Colecciones
Colección Publicaciones Científicas
Revisa las metricas alternativas de Almetrics
Revisa las citaciones de Dimensions