Carbon metabolism influenced for promoters and temperature used in the heterologous protein production using Pichia pastoris yeast
| Primer Autor |
Farias, Jorge G.
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| Co-autores |
Zepeda, Andrea B.#Pessoa, Adalberto, Jr.
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| Título |
Carbon metabolism influenced for promoters and temperature used in the heterologous protein production using Pichia pastoris yeast
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| Editorial |
SPRINGER
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| Revista |
BRAZILIAN JOURNAL OF MICROBIOLOGY
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| Lenguaje |
en
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| Resumen |
Nowadays, it is necessary to search for different high-scale production strategies to produce recombinant proteins of economic interest. Only a few microorganisms are industrially relevant for recombinant protein production: methylotrophic yeasts are known to use methanol efficiently as the sole carbon and energy source. Pichia pastoris is a methylotrophic yeast characterized as being an economical, fast and effective system for heterologous protein expression. Many factors can affect both the product and the production, including the promoter, carbon source, pH, production volume, temperature, and many others, but to control all of them most of the time is difficult and this depends on the initial selection of each variable. Therefore, this review focuses on the selection of the best promoter in the recombination process, considering different inductors, and the temperature as a culture medium variable in methylotrophic Pichia pastoris yeast. The goal is to understand the effects associated with different factors that influence its cell metabolism and to reach the construction of an expression system that fulfills the requirements of the yeast, presenting an optimal growth and development in batch, fed-batch or continuous cultures, and at the same time improve its yield in heterologous protein production. (C) 2018 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.
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| Tipo de Recurso |
Artículo de revisión
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| Description |
The authors acknowledge the financial support of the Comision Nacional de Inyestigacion Cientifica y Tecnologica de Chile (CONICYT No 21110306) doctoral fellowship awarded to AZP (Chile), and 2012/50210-9 FAPESP (Sao Paulo Research Foundation, Brazil) (AP and JGF).
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| doi |
10.1016/j.bjm.2018.03.010
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| Formato Recurso |
pdf
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| Palabras Claves |
Methylotrophic yeast# Carbon source# Inducible promoter# Alcohol oxidase
Methylotrophic yeast# Carbon source# Inducible promoter# Alcohol oxidase
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| Ubicación del archivo |
http://dx.doi.org/10.1016/j.bjm.2018.03.010
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| Categoría OCDE |
Microbiology
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| Materias |
Levadura metilotrófica# Fuente de carbono# Promotor inducible# alcohol oxidasa
Levadura metilotrófica# Fuente de carbono# Promotor inducible# alcohol oxidasa
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| Id de Web of Science |
WOS:000451915500017
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| Título de la cita (Recomendado-único) |
Carbon metabolism influenced for promoters and temperature used in the heterologous protein production using <i>Pichia pastoris</i> yeast
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| Identificador del recurso (Mandatado-único) |
Artículo de revisión
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| Versión del recurso (Recomendado-único) |
version publicada
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| Editorial |
SPRINGER
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| Revista/Libro |
BRAZILIAN JOURNAL OF MICROBIOLOGY
|
| Categoría WOS |
Microbiología
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| ISSN |
1517-8382
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| Idioma |
en
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| Referencia del Financiador (Mandatado si es aplicable-repetible) |
ANID CONICYT 21110306#FAPESP 2012/50210-9
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| Descripción |
The authors acknowledge the financial support of the Comision Nacional de Inyestigacion Cientifica y Tecnologica de Chile (CONICYT No 21110306) doctoral fellowship awarded to AZP (Chile), and 2012/50210-9 FAPESP (Sao Paulo Research Foundation, Brazil) (AP and JGF).
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| Formato |
pdf
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| Tipo de ruta |
hibrida#verde
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| Access Rights |
acceso abierto
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| Derechos de acceso |
acceso abierto
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| License |
CC BY-NC-ND 4.0
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