Repositorio UFRO · Omeka S

Carbon metabolism influenced for promoters and temperature used in the heterologous protein production using Pichia pastoris yeast

Primer Autor	Farias, Jorge G.
Co-autores	Zepeda, Andrea B.#Pessoa, Adalberto, Jr.
Título	Carbon metabolism influenced for promoters and temperature used in the heterologous protein production using Pichia pastoris yeast
Editorial	SPRINGER
Revista	BRAZILIAN JOURNAL OF MICROBIOLOGY
Lenguaje	en
Resumen	Nowadays, it is necessary to search for different high-scale production strategies to produce recombinant proteins of economic interest. Only a few microorganisms are industrially relevant for recombinant protein production: methylotrophic yeasts are known to use methanol efficiently as the sole carbon and energy source. Pichia pastoris is a methylotrophic yeast characterized as being an economical, fast and effective system for heterologous protein expression. Many factors can affect both the product and the production, including the promoter, carbon source, pH, production volume, temperature, and many others, but to control all of them most of the time is difficult and this depends on the initial selection of each variable. Therefore, this review focuses on the selection of the best promoter in the recombination process, considering different inductors, and the temperature as a culture medium variable in methylotrophic Pichia pastoris yeast. The goal is to understand the effects associated with different factors that influence its cell metabolism and to reach the construction of an expression system that fulfills the requirements of the yeast, presenting an optimal growth and development in batch, fed-batch or continuous cultures, and at the same time improve its yield in heterologous protein production. (C) 2018 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.
Tipo de Recurso	Artículo de revisión
Description	The authors acknowledge the financial support of the Comision Nacional de Inyestigacion Cientifica y Tecnologica de Chile (CONICYT No 21110306) doctoral fellowship awarded to AZP (Chile), and 2012/50210-9 FAPESP (Sao Paulo Research Foundation, Brazil) (AP and JGF). Los autores agradecen el apoyo financiero de la beca de doctorado de la Comisión Nacional de Investigación Científica y Tecnológica de Chile (CONICYT No 21110306) otorgada a AZP (Chile) y 2012/50210-9 FAPESP (Fundación de Investigación de Sao Paulo, Brasil) (AP y JGF).
doi	10.1016/j.bjm.2018.03.010
Formato Recurso	pdf
Palabras Claves	Methylotrophic yeast# Carbon source# Inducible promoter# Alcohol oxidase
Ubicación del archivo	http://dx.doi.org/10.1016/j.bjm.2018.03.010
Categoría OCDE	Microbiology
Materias	Levadura metilotrófica# Fuente de carbono# Promotor inducible# alcohol oxidasa
Disciplinas de la OCDE	Biotecnología Industrial Microbiología Biología Molecular
Versión del recurso (Recomendado-único)	version publicada
Tipo de ruta	hibrida verde
Access Rights	acceso abierto
Derechos de acceso	acceso abierto
Id de Web of Science	WOS:000451915500017
Título de la cita (Recomendado-único)	Carbon metabolism influenced for promoters and temperature used in the heterologous protein production using <i>Pichia pastoris</i> yeast
Identificador del recurso (Mandatado-único)	Artículo de revisión
Editorial	SPRINGER
Revista/Libro	BRAZILIAN JOURNAL OF MICROBIOLOGY
Categoría WOS	Microbiología
ISSN	1517-8382
Idioma	en
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID CONICYT 21110306#FAPESP 2012/50210-9 ANID CONICYT 21110306
Descripción	The authors acknowledge the financial support of the Comision Nacional de Inyestigacion Cientifica y Tecnologica de Chile (CONICYT No 21110306) doctoral fellowship awarded to AZP (Chile), and 2012/50210-9 FAPESP (Sao Paulo Research Foundation, Brazil) (AP and JGF).
Formato	pdf
License	CC BY-NC-ND 4.0