Carbon metabolism influenced for promoters and temperature used in the heterologous protein production using Pichia pastoris yeast

Primer Autor
Farias, Jorge G.
Co-autores
Zepeda, Andrea B.#Pessoa, Adalberto, Jr.
Título
Carbon metabolism influenced for promoters and temperature used in the heterologous protein production using Pichia pastoris yeast
Editorial
SPRINGER
Revista
BRAZILIAN JOURNAL OF MICROBIOLOGY
Lenguaje
en
Resumen
Nowadays, it is necessary to search for different high-scale production strategies to produce recombinant proteins of economic interest. Only a few microorganisms are industrially relevant for recombinant protein production: methylotrophic yeasts are known to use methanol efficiently as the sole carbon and energy source. Pichia pastoris is a methylotrophic yeast characterized as being an economical, fast and effective system for heterologous protein expression. Many factors can affect both the product and the production, including the promoter, carbon source, pH, production volume, temperature, and many others, but to control all of them most of the time is difficult and this depends on the initial selection of each variable. Therefore, this review focuses on the selection of the best promoter in the recombination process, considering different inductors, and the temperature as a culture medium variable in methylotrophic Pichia pastoris yeast. The goal is to understand the effects associated with different factors that influence its cell metabolism and to reach the construction of an expression system that fulfills the requirements of the yeast, presenting an optimal growth and development in batch, fed-batch or continuous cultures, and at the same time improve its yield in heterologous protein production. (C) 2018 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.
Tipo de Recurso
Artículo de revisión
Description
The authors acknowledge the financial support of the Comision Nacional de Inyestigacion Cientifica y Tecnologica de Chile (CONICYT No 21110306) doctoral fellowship awarded to AZP (Chile), and 2012/50210-9 FAPESP (Sao Paulo Research Foundation, Brazil) (AP and JGF).
Los autores agradecen el apoyo financiero de la beca de doctorado de la Comisión Nacional de Investigación Científica y Tecnológica de Chile (CONICYT No 21110306) otorgada a AZP (Chile) y 2012/50210-9 FAPESP (Fundación de Investigación de Sao Paulo, Brasil) (AP y JGF).
doi
10.1016/j.bjm.2018.03.010
Formato Recurso
pdf
Palabras Claves
Methylotrophic yeast# Carbon source# Inducible promoter# Alcohol oxidase
Ubicación del archivo
http://dx.doi.org/10.1016/j.bjm.2018.03.010
Categoría OCDE
Microbiology
Materias
Levadura metilotrófica# Fuente de carbono# Promotor inducible# alcohol oxidasa
Disciplinas de la OCDE
Biotecnología Industrial
Microbiología
Biología Molecular
Id de Web of Science
WOS:000451915500017
Título de la cita (Recomendado-único)
Carbon metabolism influenced for promoters and temperature used in the heterologous protein production using <i>Pichia pastoris</i> yeast
Identificador del recurso (Mandatado-único)
Artículo de revisión
Versión del recurso (Recomendado-único)
version publicada
Editorial
SPRINGER
Revista/Libro
BRAZILIAN JOURNAL OF MICROBIOLOGY
Categoría WOS
Microbiología
ISSN
1517-8382
Idioma
en
Referencia del Financiador (Mandatado si es aplicable-repetible)
ANID CONICYT 21110306#FAPESP 2012/50210-9
ANID CONICYT 21110306
Descripción
The authors acknowledge the financial support of the Comision Nacional de Inyestigacion Cientifica y Tecnologica de Chile (CONICYT No 21110306) doctoral fellowship awarded to AZP (Chile), and 2012/50210-9 FAPESP (Sao Paulo Research Foundation, Brazil) (AP and JGF).
Formato
pdf
Tipo de ruta
hibrida#verde
Access Rights
acceso abierto
Derechos de acceso
acceso abierto
License
CC BY-NC-ND 4.0
Colecciones
Colección Publicaciones Científicas
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