Repositorio
Institucional

Acuna, Jacquelinne J.

Rilling, Joaquin I.#Sadowsky, Michael J.#Jorquera, Milko A.

Putative Nitrogen-Fixing Bacteria Associated With the Rhizosphere and Root Endosphere of Wheat Plants Grown in an Andisol From Southern Chile

FRONTIERS MEDIA SA

FRONTIERS IN MICROBIOLOGY

en

Acidic ash derived volcanic soils (Andisols) support 50% of cereal production in Chile. Nitrogen (N) is essential for cereal crops and commonly added as urea with consequent environmental concerns due to leaching. Despite the relevance of N to plant growth, few studies have focused on understanding the application, management and ecological role of N-2-fixing bacterial populations as tool for improve the N nutrition of cereal crops in Chile. It is known that N-2-fixing bacteria commonly inhabits diverse plant compartments (e.g., rhizosphere and root endosphere) where they can supply N for plant growth. Here, we used culture-independent and dependent approaches to characterize and compare the putative N-2-fixing bacteria associated with the rhizosphere and root endosphere of wheat plants grown in an Andisol from southern Chile. Our results showed significantly greater bacterial loads in the rhizosphere than the root endosphere. Quantitative PCR results indicated that the copy number of the 16S rRNA gene ranged from 10(12)similar to 10(13) and 10(7)similar to 10(8) g(-1) sample in rhizosphere and root endosphere, respectively. The nifH gene copy number ranged from 10(5)similar to 10(6) and 10(5) g(-1) sample in rhizosphere and root endosphere, respectively. The total culturable bacteria number ranged from 10(9)similar to 10(10) and 10(7)similar to 10(8) CFU g(-1) sample in rhizosphere and 10(4)similar to 10(5) and 10(4) CFU g(-1) sample in root endosphere using LB and NM-1 media, respectively. Indirect counts of putative N-2-fixing bacteria were 10(3) and 10(2)similar to 10(3) CFU g(-1 )sample in rhizosphere and root endosphere using NFb medium, respectively. Sequencing of 16S rRNA genes from randomly selected putative N-2-fixing bacteria revealed the presence of members of Proteobacteria (Bosea and Roseomonas), Actinobacteria (Georgenia, Mycobacterium, Microbacterium, Leifsonia, and Arthrobacter), Bacteroidetes (Chitinophaga) and Firmicutes (Bacillus and Psychrobacillus) taxa. Differences in 16S rRNA and putative nifH-containing bacterial communities between rhizosphere and root endosphere were shown by denaturing gradient gel electrophoresis (DGGE). This study shows a compartmentalization between rhizosphere and root endosphere for both the abundance and diversity of total (16S rRNA) and putative N-2-fixing bacterial communities on wheat plants grown in Chilean Andisols. This information can be relevant for the design and application of agronomic strategies to enhance sustainable N-utilization in cereal crops in Chile.

Artículo original

This study was funded in part by grants from the Chilean government: FONDECYT Regular No. 1160302 and FONDECYT Initiation into Research No. 11160112, and by the University of Minnesota Agricultural Experiment Station (to MS). Joaquin Rilling acknowledges the support of CONICYT Doctoral Scholarship program, Grant No. 21150794.

Este estudio fue financiado en parte por subvenciones del gobierno chileno: FONDECYT Regular N.° 1160302 y FONDECYT Iniciación a la Investigación N.° 11160112, y por la Estación Experimental Agrícola de la Universidad de Minnesota (a la maestría). Joaquín Rilling agradece el apoyo del programa de Becas de Doctorado de CONICYT, Subvención N.° 21150794.

10.3389/fmicb.2018.02710

pdf

Andisol# root endosphere# N-2-fixing bacteria# rhizosphere# wheat

http://dx.doi.org/10.3389/fmicb.2018.02710

Microbiology

Andisol# endosfera de la raíz# bacterias fijadoras de N-2# rizosfera# trigo

Agronomía

Ciencias del suelo

Microbiología

WOS:000450665900001

Putative Nitrogen-Fixing Bacteria Associated With the Rhizosphere and Root Endosphere of Wheat Plants Grown in an Andisol From Southern Chile

Artículo original

version publicada

FRONTIERS MEDIA SA

FRONTIERS IN MICROBIOLOGY

Microbiología

1664-302X

en

ANID FONDECYT 1160302#ANID FONDECYT Initiation 11160112#ANID CONICYT Doctoral Scholarship 21150794

ANID FONDECYT 1160302

ANID FONDECYT INICIACION 11160112

University of Minnesota Agricultural Experiment Station

ANID CONICYT Doctoral Scholarship 21150794

This study was funded in part by grants from the Chilean government: FONDECYT Regular No. 1160302 and FONDECYT Initiation into Research No. 11160112, and by the University of Minnesota Agricultural Experiment Station (to MS). Joaquin Rilling acknowledges the support of CONICYT Doctoral Scholarship program, Grant No. 21150794.

pdf

dorada#verde

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acceso abierto

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