Repositorio
Institucional

Farias, Jorge G.

Zepeda, Andrea B.#Figueroa, Carolina A.#Pessoa, Adalberto

Free fatty acids reduce metabolic stress and favor a stable production of heterologous proteins in Pichia pastoris

SOC BRASILEIRA MICROBIOLOGIA

BRAZILIAN JOURNAL OF MICROBIOLOGY

en

The growth of yeasts in culture media can be affected by many factors. For example, methanol can be metabolized by other pathways to produce ethanol, which acts as an inhibitor of the heterologous protein production pathway, oxygen concentration can generate aerobic or anaerobic environments and affects the fermentation rate, and temperature affects the central carbon metabolism and stress response protein folding. The main goal of this study was determine the implication of free fatty acids on the production of heterologous proteins in different culture conditions in cultures of Pichia pastoris. We evaluated cell viability using propidium iodide by flow cytometry and thiobarbituric acid reactive substances to measure cell membrane damage. The results indicate that the use of low temperatures and low methanol concentrations favors the decrease in lipid peroxidation in the transition phase from glycerol to methanol. In addition, a temperature of 14 degrees C + 1%M provided the most stable viability. By contrast, the temperature of 18 degrees C+ 1.5%M favored the production of a higher antibody fragment concentration. In summary, these results demonstrate that the decrease in lipid peroxidation is related to an increased production of free fatty acids. (C) 2018 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.

Artículo original

The authors acknowledge the financial support of the Comision Nacional de Investigacion Cientifica y Tecnologica de Chile (CONICYT) doctoral fellowship awarded to CFT and AZP (Chile), and 2012/50210-9 FAPESP (Sao Paulo Research Foundation, Brazil) (AP and JGF). None of the authors have any conflict of interest in the manuscript.

Los autores agradecen el apoyo financiero de la beca doctoral de la Comisión Nacional de Investigación Científica y Tecnológica de Chile (CONICYT) otorgada a CFT y AZP (Chile), y la beca 2012/50210-9 de la FAPESP (Fundación de Apoyo a la Investigación Científica de São Paulo, Brasil) (AP y JGF). Ninguno de los autores tiene ningún conflicto de intereses en relación con el manuscrito.

10.1016/j.bjm.2018.03.008

pdf

Lipid peroxidation# Pyruvate pathway# Yeast

http://dx.doi.org/10.1016/j.bjm.2018.03.008

Microbiology

Peroxidación lipídica# Vía del piruvato# Levadura

Biotecnología Industrial

Microbiología

Bioquímica

WOS:000447893000022

Free fatty acids reduce metabolic stress and favor a stable production of heterologous proteins in <i>Pichia pastoris</i>

Artículo original

version publicada

SOC BRASILEIRA MICROBIOLOGIA

BRAZILIAN JOURNAL OF MICROBIOLOGY

Microbiología

1517-8382

en

ANID CONICYT#FAPESP 012/50210-9

ANID CONICYT doctoral

FAPESP 2012/50210-9

The authors acknowledge the financial support of the Comision Nacional de Investigacion Cientifica y Tecnologica de Chile (CONICYT) doctoral fellowship awarded to CFT and AZP (Chile), and 2012/50210-9 FAPESP (Sao Paulo Research Foundation, Brazil) (AP and JGF). None of the authors have any conflict of interest in the manuscript.

pdf

hibrida#dorada

acceso abierto

acceso abierto

CC BY-NC-ND 4.0

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