Repositorio
Institucional

del Sol, Mariano

Schencke, Carolina#Sandoval, Cristian#Vasquez, Belgica

Quantitative analysis of dermal scars in deep skin burns treated with Ulmo honey supplemented with ascorbic acid

E-CENTURY PUBLISHING CORP

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE

en

Successful repair of burn wounds requires not only the participation of cells, such as granulocytes and fibroblasts, but also non-cellular elements including collagen, which plays a crucial role as a structural and regulatory molecule of scar tissue. Given that honey and ascorbic acid have presented great therapeutic potential at a cellular and structural level, experimental studies have proposed to combine them for the treatment of wounds. The aim of this study was to evaluate the morphoquantitative characteristics of polymorphonuclear cells (PMN), fibroblasts and types I and III collagen in burn wounds treated with Ulmo honey (Eucryphia cordifolia) supplemented with ascorbic acid. Fifteen healthy adult guinea pigs (Cavia porcellus) were divided into three groups: Positive Control (C+), experimental non-supplemented (H), and experimental supplemented (SH) Ulmo honey. The animals were treated daily until biopsies were performed on day 10 of treatment. Number density per area (N-A = mm(-2)) and volume density (V-V = %) of PMN, N-A, V-V and surface density (S-V = mm(-1)) of fibroblasts, and types I and III collagen were evaluated. A one-way ANOVA was used (P < 0.05). Although all groups were in a proliferative stage, the SH group showed morphoquantitative characteristics suggestive of more advanced healing processes with lower stereological PMN values (N-A = 228.823 +/- 174.883 and V-V = 1.050 +/- 0.940). However, N-A (4252.025 +/- 1041.751), V-V (20.400 +/- 5.897) and S-V (100.876 +/- 29.431) of fibroblasts and types I and III collagen content were higher in this group (P < 0.001). We conclude that burn wounds treated with Ulmo honey supplemented with ascorbic acid show a more effective healing process than non-supplemented honey.

Artículo original

Direccion de Investigacion de Universidad de La Frontera (Grant No. DI13-0044), Chile. Corporacion de Fomento de la Produccion (CORFO), (Grant No. 13IDL2-23290), Chile. CONICYT-PCHA/Doctorado Nacional/2014-21141130.

Dirección de Investigación de la Universidad de La Frontera (Subvención No. DI13-0044), Chile. Corporación de Fomento de la Producción (CORFO), (Subvención No. 13IDL2-23290), Chile. CONICYT-PCHA/Doctorado Nacional/2014-21141130.

pdf

Ascorbic acid# collagen content# healing# Ulmo honey# stereology

https://e-century.us/files/ijcem/11/3/ijcem0063974.pdf

Medicine, Research & Experimental

ácido ascórbico# contenido de colágeno# cicatrización# Miel de Ulmo# estereología

Biología Celular

Otros Temas de Medicina Clínica

Bioquímica

WOS:000429006600116

Quantitative analysis of dermal scars in deep skin burns treated with Ulmo honey supplemented with ascorbic acid

Artículo original

version publicada

E-CENTURY PUBLISHING CORP

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE

Medicina, Investigación y Experimental

1940-5901

en

UFRO DI13-0044#CORFO 13IDL2-23290#ANID CONICYT-PCHA/Doctorado Nacional/2014 21141130.

UFRO DI13-0044

CORFO 13IDL2-23290

ANID CONICYT-PCHA/Doctorado Nacional 2014 21141130

Direccion de Investigacion de Universidad de La Frontera (Grant No. DI13-0044), Chile. Corporacion de Fomento de la Produccion (CORFO), (Grant No. 13IDL2-23290), Chile. CONICYT-PCHA/Doctorado Nacional/2014-21141130.

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