Repositorio
Institucional

Herrero-Fresno, Ana

Espinel, Irene Cartas#Spiegelhauer, Malene Roed#Guerra, Priscila Regina#Andersen, Karsten Wiber#Olsen, John Elmerdahl

The Homolog of the Gene bstA of the BTP1 Phage from Salmonella enterica Serovar Typhimurium ST313 Is an Antivirulence Gene in Salmonella enterica Serovar Dublin

AMER SOC MICROBIOLOGY

INFECTION AND IMMUNITY

en

In a previous study, a novel virulence gene, bstA, identified in a Salmonella enterica serovar Typhimurium sequence type 313 (ST313) strain was found to be conserved in all published Salmonella enterica serovar Dublin genomes. In order to analyze the role of this gene in the host-pathogen interaction in S. Dublin, a mutant where this gene was deleted (S. Dublin Delta bstA) and a mutant which was further genetically complemented with bstA (S. Dublin 3246-C) were constructed and tested in models of in vitro and in vivo infection as well as during growth competition assays in M9 medium, Luria-Bertani broth, and cattle blood. In contrast to the results obtained for a strain of S. Typhimurium ST313, the lack of bstA was found to be associated with increased virulence in S. Dublin. Thus, S. Dublin Delta bstA showed higher levels of uptake than the wild-type strain during infection of mouse and cattle macrophages and higher net replication within human THP-1 cells. Furthermore, during mouse infections, S. Dublin Delta bstA was more virulent than the wild type following a single intraperitoneal infection and showed an increased competitive index during competitive infection assays. Deletion of bstA did not affect either the amount of cytokines released by THP-1 macrophages or the cytotoxicity toward these cells. The histology of the livers and spleens of mice infected with the wild-type strain and the S. Dublin Delta bstA mutant revealed similar levels of inflammation between the two groups. The gene was not important for adherence to or invasion of human epithelial cells and did not influence bacterial growth in rich medium, minimal medium, or cattle blood. In conclusion, a lack of bstA affects the pathogenicity of S. Dublin by decreasing its virulence. Therefore, it might be regarded as an antivirulence gene in this serovar.

Artículo original

10.1128/IAI.00784-17

pdf

Salmonella enterica serovar Dublin# bstA# antivirulence# infection# cell lines# cattle blood# mouse

http://dx.doi.org/10.1128/IAI.00784-17

Immunology# Infectious Diseases

Salmonella enterica serovar Dublin# bstA# antivirulencia# infección# líneas celulares# sangre de ganado# ratón

Microbiología

Genética y Herencia

Patología

WOS:000418239900011

The Homolog of the Gene <i>bstA</i> of the BTP1 Phage from <i>Salmonella enterica</i> Serovar Typhimurium ST313 Is an Antivirulence Gene in <i>Salmonella enterica</i> Serovar Dublin

Artículo original

version publicada

AMER SOC MICROBIOLOGY

INFECTION AND IMMUNITY

Inmunología# Enfermedades infecciosas

0019-9567

en

pdf

dorada

acceso abierto

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