The Homolog of the Gene bstA of the BTP1 Phage from Salmonella enterica Serovar Typhimurium ST313 Is an Antivirulence Gene in Salmonella enterica Serovar Dublin

Primer Autor
Herrero-Fresno, Ana
Co-autores
Espinel, Irene Cartas#Spiegelhauer, Malene Roed#Guerra, Priscila Regina#Andersen, Karsten Wiber#Olsen, John Elmerdahl
Título
The Homolog of the Gene bstA of the BTP1 Phage from Salmonella enterica Serovar Typhimurium ST313 Is an Antivirulence Gene in Salmonella enterica Serovar Dublin
Editorial
AMER SOC MICROBIOLOGY
Revista
INFECTION AND IMMUNITY
Lenguaje
en
Resumen
In a previous study, a novel virulence gene, bstA, identified in a Salmonella enterica serovar Typhimurium sequence type 313 (ST313) strain was found to be conserved in all published Salmonella enterica serovar Dublin genomes. In order to analyze the role of this gene in the host-pathogen interaction in S. Dublin, a mutant where this gene was deleted (S. Dublin Delta bstA) and a mutant which was further genetically complemented with bstA (S. Dublin 3246-C) were constructed and tested in models of in vitro and in vivo infection as well as during growth competition assays in M9 medium, Luria-Bertani broth, and cattle blood. In contrast to the results obtained for a strain of S. Typhimurium ST313, the lack of bstA was found to be associated with increased virulence in S. Dublin. Thus, S. Dublin Delta bstA showed higher levels of uptake than the wild-type strain during infection of mouse and cattle macrophages and higher net replication within human THP-1 cells. Furthermore, during mouse infections, S. Dublin Delta bstA was more virulent than the wild type following a single intraperitoneal infection and showed an increased competitive index during competitive infection assays. Deletion of bstA did not affect either the amount of cytokines released by THP-1 macrophages or the cytotoxicity toward these cells. The histology of the livers and spleens of mice infected with the wild-type strain and the S. Dublin Delta bstA mutant revealed similar levels of inflammation between the two groups. The gene was not important for adherence to or invasion of human epithelial cells and did not influence bacterial growth in rich medium, minimal medium, or cattle blood. In conclusion, a lack of bstA affects the pathogenicity of S. Dublin by decreasing its virulence. Therefore, it might be regarded as an antivirulence gene in this serovar.
Tipo de Recurso
Artículo original
doi
10.1128/IAI.00784-17
Formato Recurso
pdf
Palabras Claves
Salmonella enterica serovar Dublin# bstA# antivirulence# infection# cell lines# cattle blood# mouse
Ubicación del archivo
http://dx.doi.org/10.1128/IAI.00784-17
Categoría OCDE
Immunology# Infectious Diseases
Materias
Salmonella enterica serovar Dublin# bstA# antivirulencia# infección# líneas celulares# sangre de ganado# ratón
Disciplinas de la OCDE
Microbiología
Genética y Herencia
Patología
Id de Web of Science
WOS:000418239900011
Título de la cita (Recomendado-único)
The Homolog of the Gene <i>bstA</i> of the BTP1 Phage from <i>Salmonella enterica</i> Serovar Typhimurium ST313 Is an Antivirulence Gene in <i>Salmonella enterica</i> Serovar Dublin
Identificador del recurso (Mandatado-único)
Artículo original
Versión del recurso (Recomendado-único)
version publicada
Editorial
AMER SOC MICROBIOLOGY
Revista/Libro
INFECTION AND IMMUNITY
Categoría WOS
Inmunología# Enfermedades infecciosas
ISSN
0019-9567
Idioma
en
Formato
pdf
Tipo de ruta
dorada
Access Rights
acceso abierto
Derechos de acceso
acceso abierto
Página de inicio (Recomendado-único)
496
Página final (Recomendado-único)
506
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