Gallic acid loaded PEO-core/zein-shell nanofibers for chemopreventive action on gallbladder cancer cells
| Primer Autor |
Acevedo, Francisca
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| Co-autores |
Hermosilla, Jeyson#Sanhueza, Claudia#Mora-Lagos, Barbara#Fuentes, Irma#Rubilar, Monica#Concheiro, Angel#Alvarez-Lorenzo, Carmen
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| Título |
Gallic acid loaded PEO-core/zein-shell nanofibers for chemopreventive action on gallbladder cancer cells
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| Editorial |
ELSEVIER SCIENCE BV
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| Revista |
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
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| Lenguaje |
en
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| Resumen |
Coaxial electrospinning was used to develop gallic acid (GA) loaded poly(ethylene oxide)/zein nanofibers in order to improve its chemopreventive action on human gallbladder cancer cells. Using a Plackett-Burman design, the effects of poly(ethylene oxide) and zein concentration and applied voltage on the diameter and morphology index of nanofibers were investigated. Coaxial nanofibers were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). GA loading efficiency as high as 77% was obtained under optimal process conditions. The coaxial nanofibers controlled GA release in acid and neutral pH medium. Cytotoxicity and reactive oxygen species (ROS) production on gallbladder cancer cell lines GB-d1 and NOZ in the presence of GA-nanofibers were assessed. GA-nanofibers triggered an increase in the cellular cytotoxicity compared with free GA on GB-d1 and NOZ cells. Statistically significant differences were found in ROS levels of GA-nanofibers compared with free GA on NOZ cells. Differently, ROS production on GB-d1 cell line was similar. Based on these results, the coaxial nanofibers obtained in this study under optimized operational conditions offer an alternative for the development of a GA release system with improved chemopreventive action on gallbladder cancer cells.
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| Tipo de Recurso |
Artículo original
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| Description |
Work financially supported by FONDECYT (Project 11140127) Chile, MICINN (Grant SAF2014-52632-R) Spain, and FEDER.
Trabajo financiado por FONDECYT (Proyecto 11140127) Chile, MICINN (Beca SAF2014-52632-R) España y FEDER.
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| doi |
10.1016/j.ejps.2018.04.009
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| Formato Recurso |
pdf
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| Palabras Claves |
Biopolymers# Zein# Poly(ethylene oxide)# Coaxial electrospinning# Gallbladder cancer cells# Reactive oxygen species
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| Ubicación del archivo |
http://dx.doi.org/10.1016/j.ejps.2018.04.009
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| Categoría OCDE |
Pharmacology & Pharmacy
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| Materias |
Biopolímeros# zeín# Poli(óxido de etileno)# Electrohilado coaxial# Células cancerosas de la vesícula biliar# Especies reactivas de oxígeno.
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| Disciplinas de la OCDE |
Biotecnología Relacionada con la Salud
Ingeniería Química
Oncología
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| Id de Web of Science |
WOS:000432573700006
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| Título de la cita (Recomendado-único) |
Gallic acid loaded PEO-core/zein-shell nanofibers for chemopreventive action on gallbladder cancer cells
|
| Identificador del recurso (Mandatado-único) |
Artículo original
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| Versión del recurso (Recomendado-único) |
version publicada
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| Editorial |
ELSEVIER SCIENCE BV
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| Revista/Libro |
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
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| Categoría WOS |
Farmacología y farmacia
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| ISSN |
0928-0987
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| Idioma |
en
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| Referencia del Financiador (Mandatado si es aplicable-repetible) |
ANID FONDECYT 11140127#MICINN SAF2014-52632-R
ANID FONDECYT 11140127
MICINN SAF2014-52632-R
FEDER
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| Descripción |
Work financially supported by FONDECYT (Project 11140127) Chile, MICINN (Grant SAF2014-52632-R) Spain, and FEDER.
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| Formato |
pdf
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| Tipo de ruta |
suscripción#verde
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| Access Rights |
metadata
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| Derechos de acceso |
metadata
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| Página de inicio (Recomendado-único) |
304
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| Página final (Recomendado-único) |
317
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