Repositorio UFRO · Omeka S

Gallic acid loaded PEO-core/zein-shell nanofibers for chemopreventive action on gallbladder cancer cells

Primer Autor	Acevedo, Francisca
Co-autores	Hermosilla, Jeyson#Sanhueza, Claudia#Mora-Lagos, Barbara#Fuentes, Irma#Rubilar, Monica#Concheiro, Angel#Alvarez-Lorenzo, Carmen
Título	Gallic acid loaded PEO-core/zein-shell nanofibers for chemopreventive action on gallbladder cancer cells
Editorial	ELSEVIER SCIENCE BV
Revista	EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
Lenguaje	en
Resumen	Coaxial electrospinning was used to develop gallic acid (GA) loaded poly(ethylene oxide)/zein nanofibers in order to improve its chemopreventive action on human gallbladder cancer cells. Using a Plackett-Burman design, the effects of poly(ethylene oxide) and zein concentration and applied voltage on the diameter and morphology index of nanofibers were investigated. Coaxial nanofibers were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). GA loading efficiency as high as 77% was obtained under optimal process conditions. The coaxial nanofibers controlled GA release in acid and neutral pH medium. Cytotoxicity and reactive oxygen species (ROS) production on gallbladder cancer cell lines GB-d1 and NOZ in the presence of GA-nanofibers were assessed. GA-nanofibers triggered an increase in the cellular cytotoxicity compared with free GA on GB-d1 and NOZ cells. Statistically significant differences were found in ROS levels of GA-nanofibers compared with free GA on NOZ cells. Differently, ROS production on GB-d1 cell line was similar. Based on these results, the coaxial nanofibers obtained in this study under optimized operational conditions offer an alternative for the development of a GA release system with improved chemopreventive action on gallbladder cancer cells.
Tipo de Recurso	Artículo original
Description	Work financially supported by FONDECYT (Project 11140127) Chile, MICINN (Grant SAF2014-52632-R) Spain, and FEDER. Trabajo financiado por FONDECYT (Proyecto 11140127) Chile, MICINN (Beca SAF2014-52632-R) España y FEDER.
doi	10.1016/j.ejps.2018.04.009
Formato Recurso	pdf
Palabras Claves	Biopolymers# Zein# Poly(ethylene oxide)# Coaxial electrospinning# Gallbladder cancer cells# Reactive oxygen species
Ubicación del archivo	http://dx.doi.org/10.1016/j.ejps.2018.04.009
Categoría OCDE	Pharmacology & Pharmacy
Materias	Biopolímeros# zeín# Poli(óxido de etileno)# Electrohilado coaxial# Células cancerosas de la vesícula biliar# Especies reactivas de oxígeno.
Disciplinas de la OCDE	Biotecnología Relacionada con la Salud Ingeniería Química Oncología
Id de Web of Science	WOS:000432573700006
Título de la cita (Recomendado-único)	Gallic acid loaded PEO-core/zein-shell nanofibers for chemopreventive action on gallbladder cancer cells
Identificador del recurso (Mandatado-único)	Artículo original
Versión del recurso (Recomendado-único)	version publicada
Editorial	ELSEVIER SCIENCE BV
Revista/Libro	EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
Categoría WOS	Farmacología y farmacia
ISSN	0928-0987
Idioma	en
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID FONDECYT 11140127#MICINN SAF2014-52632-R ANID FONDECYT 11140127 MICINN SAF2014-52632-R FEDER
Descripción	Work financially supported by FONDECYT (Project 11140127) Chile, MICINN (Grant SAF2014-52632-R) Spain, and FEDER.
Formato	pdf
Tipo de ruta	suscripción#verde
Access Rights	metadata
Derechos de acceso	metadata
Página de inicio (Recomendado-único)	304
Página final (Recomendado-único)	317