Hypoglycemic effect of Berberis microphylla G Forst root extract

Primer Autor
Salazar, Luis A.
Co-autores
Furrianca, Maria C.#Alvear, Marysol#Zambrano, Tomas#Fajardo, Victor
Título
Hypoglycemic effect of Berberis microphylla G Forst root extract
Editorial
PHARMACOTHERAPY GROUP
Revista
TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
Lenguaje
en
Resumen
Purpose: To evaluate the effect of the root extract of Berberis microphylla on glucose uptake and AMPK-activated protein kinase (AMPK) activity in non-resistant and insulin-resistant HepG2 cells. Methods: B. microphylla root was extracted with absolute ethanol, filtered, concentrated and lyophilized. Subsequently, liver cells, HepG2 (resistant and non-insulin resistant), were exposed for 24 h to different concentrations of the extract (10, 5, 2.5 and 1.25 x 10(-3) mu g/mu L) to determine the stimulation of glucose uptake and phosphorylation of AMPK. Results: In HepG2 cells without resistance exposed to B. microphylla root extract, glucose uptake varied from 34 to 59 % of the available glucose while AMPK phosphorylation was 1.9 to 3.6 times the phosphorylation of the control. In insulin-resistant HepG2 cells, glucose uptake varied from 68 to 95 % of available glucose while AMPK phosphorylation was 1.8 to 3.3 times the phosphorylation of the control. Conclusion: The root extract of B. microphylla possesses hypoglycemic effects and stimulates glucose uptake in HepG2 cells with and without resistance by activating AMPK protein.
Tipo de Recurso
Artículo original
Description
The authors would like to thank the Advanced Human Capital Formation Program, CONICYT-CHILE for their financial support of Maria Cristina Furrianca Llaneza during her National Doctorate fellow (No. 24121475).
Los autores agradecen al Programa de Formación de Capital Humano Avanzado, CONICYT-CHILE por el apoyo financiero a María Cristina Furrianca Llaneza durante su Doctorado Nacional (N° 24121475).
doi
10.4314/tjpr.v16i9.19
Formato Recurso
pdf
Palabras Claves
Calafate# Diabetes# Antihyperglycemic effect# Phytomedicine# Berberis# Insulin resistance
Ubicación del archivo
http://dx.doi.org/10.4314/tjpr.v16i9.19
Categoría OCDE
Pharmacology & Pharmacy
Materias
Calafate# Diabetes# Efecto antihiperglucémico# Fitomedicina# bereberes# resistencia a la insulina
Disciplinas de la OCDE
Biología Celular
Farmacología y Farmacia
Bioquímica
Id de Web of Science
WOS:000418444300018
Título de la cita (Recomendado-único)
Hypoglycemic effect of Berberis microphylla G Forst root extract
Identificador del recurso (Mandatado-único)
Artículo original
Versión del recurso (Recomendado-único)
version publicada
Editorial
PHARMACOTHERAPY GROUP
Revista/Libro
TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
Categoría WOS
Farmacología y farmacia
ISSN
1596-5996
Idioma
en
Referencia del Financiador (Mandatado si es aplicable-repetible)
ANID 24121475
ANID CONICYT 24121475
Descripción
The authors would like to thank the Advanced Human Capital Formation Program, CONICYT-CHILE for their financial support of Maria Cristina Furrianca Llaneza during her National Doctorate fellow (No. 24121475).
Formato
pdf
Tipo de ruta
dorada#verde
Access Rights
acceso abierto
Derechos de acceso
acceso abierto
License
CC BY 4.0
Página de inicio (Recomendado-único)
205
Página final (Recomendado-único)
218
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