Repositorio UFRO · Omeka S

Hypoglycemic effect of Berberis microphylla G Forst root extract

Primer Autor	Salazar, Luis A.
Co-autores	Furrianca, Maria C.#Alvear, Marysol#Zambrano, Tomas#Fajardo, Victor
Título	Hypoglycemic effect of Berberis microphylla G Forst root extract
Editorial	PHARMACOTHERAPY GROUP
Revista	TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
Lenguaje	en
Resumen	Purpose: To evaluate the effect of the root extract of Berberis microphylla on glucose uptake and AMPK-activated protein kinase (AMPK) activity in non-resistant and insulin-resistant HepG2 cells. Methods: B. microphylla root was extracted with absolute ethanol, filtered, concentrated and lyophilized. Subsequently, liver cells, HepG2 (resistant and non-insulin resistant), were exposed for 24 h to different concentrations of the extract (10, 5, 2.5 and 1.25 x 10(-3) mu g/mu L) to determine the stimulation of glucose uptake and phosphorylation of AMPK. Results: In HepG2 cells without resistance exposed to B. microphylla root extract, glucose uptake varied from 34 to 59 % of the available glucose while AMPK phosphorylation was 1.9 to 3.6 times the phosphorylation of the control. In insulin-resistant HepG2 cells, glucose uptake varied from 68 to 95 % of available glucose while AMPK phosphorylation was 1.8 to 3.3 times the phosphorylation of the control. Conclusion: The root extract of B. microphylla possesses hypoglycemic effects and stimulates glucose uptake in HepG2 cells with and without resistance by activating AMPK protein.
Tipo de Recurso	Artículo original
Description	The authors would like to thank the Advanced Human Capital Formation Program, CONICYT-CHILE for their financial support of Maria Cristina Furrianca Llaneza during her National Doctorate fellow (No. 24121475). Los autores agradecen al Programa de Formación de Capital Humano Avanzado, CONICYT-CHILE por el apoyo financiero a María Cristina Furrianca Llaneza durante su Doctorado Nacional (N° 24121475).
doi	10.4314/tjpr.v16i9.19
Formato Recurso	pdf
Palabras Claves	Calafate# Diabetes# Antihyperglycemic effect# Phytomedicine# Berberis# Insulin resistance
Ubicación del archivo	http://dx.doi.org/10.4314/tjpr.v16i9.19
Categoría OCDE	Pharmacology & Pharmacy
Materias	Calafate# Diabetes# Efecto antihiperglucémico# Fitomedicina# bereberes# resistencia a la insulina
Disciplinas de la OCDE	Biología Celular Farmacología y Farmacia Bioquímica
Id de Web of Science	WOS:000418444300018
Título de la cita (Recomendado-único)	Hypoglycemic effect of Berberis microphylla G Forst root extract
Identificador del recurso (Mandatado-único)	Artículo original
Versión del recurso (Recomendado-único)	version publicada
Editorial	PHARMACOTHERAPY GROUP
Revista/Libro	TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
Categoría WOS	Farmacología y farmacia
ISSN	1596-5996
Idioma	en
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID 24121475 ANID CONICYT 24121475
Descripción	The authors would like to thank the Advanced Human Capital Formation Program, CONICYT-CHILE for their financial support of Maria Cristina Furrianca Llaneza during her National Doctorate fellow (No. 24121475).
Formato	pdf
Tipo de ruta	dorada#verde
Access Rights	acceso abierto
Derechos de acceso	acceso abierto
License	CC BY 4.0
Página de inicio (Recomendado-único)	205
Página final (Recomendado-único)	218