Repositorio
Institucional

Inostroza-Blancheteau, Claudio

de Oliveira Silva, Franklin Magnum#Duran, Fabiola#Solano, Jaime#Obata, Toshihiro#Machado, Mariana#Fernie, Alisdair R.#Reyes-Diaz, Marjorie#Nunes-Nesi, Adriano

Metabolic diversity in tuber tissues of native Chiloe potatoes and commercial cultivars of Solanum tuberosum ssp tuberosum L.

SPRINGER

METABOLOMICS

en

Introduction The native potatoes (Solanum tuberosum ssp. tuberosum L.) cultivated on Chiloe Island in southern Chile have great variability in terms of tuber shape, size, color and flavor. These traits have been preserved throughout generations due to the geographical position of Chiloe, as well as the different uses given by local farmers. Objectives The present study aimed to investigate the diversity of metabolites in skin and pulp tissues of eleven native accessions of potatoes from Chile, and evaluate the metabolite associations between tuber tissues. Methods For a deeper characterization of these accessions, we performed a comprehensive metabolic study in skin and pulp tissues of tubers, 3 months after harvesting. Specific targeted quantification of metabolites using 96 well microplates, and high-performance liquid chromatography combined with non-targeted metabolite profiling by gas chromatography time-offlight mass spectrometry were used in this study. Results We observed differential levels of antioxidant activity and phenolic compounds between skin and pulp compared to a common commercial cultivar (Desiree). In addition, we uncovered considerable metabolite variability between different tuber tissues and between native potatoes. Network correlation analysis revealed different metabolite associations among tuber tissues that indicate distinct associations between primary metabolite and anthocyanin levels, and antioxidant activity in skin and pulp tissues. Moreover, multivariate analysis lead to the grouping of native and commercial cultivars based on metabolites from both skin and pulp tissues. Conclusions As well as providing important information to potato producers and breeding programs on the levels of health relevant phytochemicals and other abundant metabolites such as starch, proteins and amino acids, this study highlights the associations of different metabolites in tuber skins and pulp, indicating the need for distinct strategies for metabolic engineering in these tissues. Furthermore, this study shows that native Chilean potato accessions have great potential as a natural source of phytochemicals.

Artículo original

This work was supported by Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG) [grant number CBB - AUC-00018-16]. Research fellowships were granted by Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) to ANN and Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG) [Grant Number CBB-BPD- 00019-16] to FMOS. ANN, MRD and CIB also acknowledge the support from the Chilean Ministerio de Educacion (MEC-CONICYT, Grant PAI80160036). The authors wish to thank the NUBIOMOL-UFV for providing the facilities for the analysis of this work, and Michael Handford (Universidad de Chile) for language support.

Este trabajo fue apoyado por la Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG) [número de subvención CBB - AUC-00018-16]. Las becas de investigación fueron otorgadas por el Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) a ANN y la Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG) [Número de subvención CBB-BPD-00019-16] a FMOS. ANN, MRD y CIB también agradecen el apoyo del Ministerio de Educación de Chile (MEC-CONICYT, Beca PAI80160036). Los autores desean agradecer a NUBIOMOL-UFV por brindar las instalaciones para el análisis de este trabajo, y a Michael Handford (Universidad de Chile) por el apoyo lingüístico.

10.1007/s11306-018-1428-7

pdf

Genetic variation# Native potatoes# Exotic germplasms# Metabolite profiling

http://dx.doi.org/10.1007/s11306-018-1428-7

Endocrinology & Metabolism

Variación genética# papas nativas# Germoplasmas exóticos# Perfil de metabolitos

Agricultura

Fitomejoramiento y protección vegetal

Bioquímica

WOS:000446497300002

Metabolic diversity in tuber tissues of native Chiloe potatoes and commercial cultivars of <i>Solanum tuberosum </i>ssp tuberosum L.

Artículo original

version publicada

SPRINGER

METABOLOMICS

Endocrinología y Metabolismo

1573-3882

en

FAPEMIG CBB - AUC-00018-16#CNPq CBB-BPD- 00019-16#ANID MEC-CONICYT PAI80160036

This work was supported by Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG) [grant number CBB - AUC-00018-16]. Research fellowships were granted by Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) to ANN and Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG) [Grant Number CBB-BPD- 00019-16] to FMOS. ANN, MRD and CIB also acknowledge the support from the Chilean Ministerio de Educacion (MEC-CONICYT, Grant PAI80160036). The authors wish to thank the NUBIOMOL-UFV for providing the facilities for the analysis of this work, and Michael Handford (Universidad de Chile) for language support.

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