Screening and Characterization of Phytases from Bacteria Isolated from Chilean Hydrothermal Environments

Primer Autor
Jorquera, Milko A.
Co-autores
Gabler, Stefanie#Inostroza, Nitza G.#Acuna, Jacquelinne J.#Campos, Marco A.#Menezes-Blackburn, Daniel#Greiner, Ralf
Título
Screening and Characterization of Phytases from Bacteria Isolated from Chilean Hydrothermal Environments
Editorial
SPRINGER
Revista
MICROBIAL ECOLOGY
Lenguaje
en
Resumen
Phytases are enzymes involved in organic phosphorus cycling in nature and widely used as feed additives in animal diets. Thermal tolerance is a desired property of phytases. The objectives of this study were to screen and characterize bacterial phytases from Chilean hydrothermal environments. In this study, 60% (30 of 63) of screened thermophilic (60 A degrees C) isolates showed phytase activity in crude protein extracts. The characterization of phytase from two selected isolates (9B and 15C) revealed that both isolates produce phytases with a pH optimum at 5.0. The temperature optimum for phytate dephosphorylation was determined to be 60 and 50 A degrees C for the phytases from the isolates 9B and 15C, respectively. Interestingly, the phytase from the isolate 15C showed a residual activity of 46% after incubation at 90 A degrees C for 20 min. The stepwise dephosphorylation of phytate by protein extracts of the isolates 9B and 15C was verified by HLPC analysis. Finally, the isolates 9B and 15C were identified by partial sequencing of the 16S rRNA gene as members of the genera Bacillus and Geobacillus, respectively.
Tipo de Recurso
Artículo original
Description
This study was supported by cooperative project (code PCCI 1-2011) funded by The National Commission for Scientific and Technological Research (CONICYT, Chile) and Deutscher Akademischer Austauschdienst German (DAAD, Germany). This work was also partial financed with funds from CONICYT-PCCI (code USA2013-0010) and The National Fund for Scientific and Technological Development (FONDECYT, Chile, projects no. 1120505 and 1160302). J.J. Acuna acknowledges FONDECYT programs (projects no. 3140620 and 11160112).
Este estudio fue financiado por el proyecto cooperativo (código PCCI 1-2011) de la Comisión Nacional de Investigación Científica y Tecnológica (CONICYT, Chile) y el DAAD (Deutscher Akademischer Austauschdienst German). Este trabajo también fue financiado parcialmente con fondos de CONICYT-PCCI (código USA2013-0010) y el Fondo Nacional para el Desarrollo Científico y Tecnológico (FONDECYT, Chile, proyectos n.º 1120505 y 1160302). J.J. Acuña reconoce los programas FONDECYT (proyectos n.º 3140620 y 11160112).
doi
10.1007/s00248-017-1057-0
Formato Recurso
pdf
Palabras Claves
Bacillus# Geobacillus# Geyser# Hot spring# Phytate# Thermophile
Ubicación del archivo
http://dx.doi.org/10.1007/s00248-017-1057-0
Categoría OCDE
Ecology# Marine & Freshwater Biology# Microbiology
Materias
Bacilo# Geobacilo# Géiser# Fuente termal# fitato# termófilo
Disciplinas de la OCDE
Biotecnología agrícola
Microbiología
Bioquímica
Id de Web of Science
WOS:000418740200010
Título de la cita (Recomendado-único)
Screening and Characterization of Phytases from Bacteria Isolated from Chilean Hydrothermal Environments
Identificador del recurso (Mandatado-único)
Artículo original
Versión del recurso (Recomendado-único)
version publicada
Editorial
SPRINGER
Revista/Libro
MICROBIAL ECOLOGY
Categoría WOS
Ecología# Biología Marina y de Agua Dulce# Microbiología# microbiología
ISSN
0095-3628
Idioma
en
Referencia del Financiador (Mandatado si es aplicable-repetible)
DAAD PCCI 1-2011#ANID PCCI 1-2011#ANID CONICYT-PCCI USA2013-0010#ANID FONDECYT 1120505#ANID FONDECYT 1160302#ANID FONDECYT 3140620#ANID FONDECYT 11160112
ANID CONICYT PCCI 1-2011
Deutscher Akademischer Austauschdienst German (DAAD, Germany)
ANID CONICYT PCCI USA2013-0010
ANID FONDECYT 1120505
ANID FONDECYT 1160302
ANID FONDECYT 3140620
ANID FONDECYT 11160112
Descripción
This study was supported by cooperative project (code PCCI 1-2011) funded by The National Commission for Scientific and Technological Research (CONICYT, Chile) and Deutscher Akademischer Austauschdienst German (DAAD, Germany). This work was also partial financed with funds from CONICYT-PCCI (code USA2013-0010) and The National Fund for Scientific and Technological Development (FONDECYT, Chile, projects no. 1120505 and 1160302). J.J. Acuna acknowledges FONDECYT programs (projects no. 3140620 and 11160112).
Formato
pdf
Tipo de ruta
hibrida
Access Rights
restringido
Derechos de acceso
restringido
Colecciones
Colección Publicaciones Científicas
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