Repositorio
Institucional

Jorquera, Milko A.

Gabler, Stefanie#Inostroza, Nitza G.#Acuna, Jacquelinne J.#Campos, Marco A.#Menezes-Blackburn, Daniel#Greiner, Ralf

Screening and Characterization of Phytases from Bacteria Isolated from Chilean Hydrothermal Environments

SPRINGER

MICROBIAL ECOLOGY

en

Phytases are enzymes involved in organic phosphorus cycling in nature and widely used as feed additives in animal diets. Thermal tolerance is a desired property of phytases. The objectives of this study were to screen and characterize bacterial phytases from Chilean hydrothermal environments. In this study, 60% (30 of 63) of screened thermophilic (60 A degrees C) isolates showed phytase activity in crude protein extracts. The characterization of phytase from two selected isolates (9B and 15C) revealed that both isolates produce phytases with a pH optimum at 5.0. The temperature optimum for phytate dephosphorylation was determined to be 60 and 50 A degrees C for the phytases from the isolates 9B and 15C, respectively. Interestingly, the phytase from the isolate 15C showed a residual activity of 46% after incubation at 90 A degrees C for 20 min. The stepwise dephosphorylation of phytate by protein extracts of the isolates 9B and 15C was verified by HLPC analysis. Finally, the isolates 9B and 15C were identified by partial sequencing of the 16S rRNA gene as members of the genera Bacillus and Geobacillus, respectively.

Artículo original

This study was supported by cooperative project (code PCCI 1-2011) funded by The National Commission for Scientific and Technological Research (CONICYT, Chile) and Deutscher Akademischer Austauschdienst German (DAAD, Germany). This work was also partial financed with funds from CONICYT-PCCI (code USA2013-0010) and The National Fund for Scientific and Technological Development (FONDECYT, Chile, projects no. 1120505 and 1160302). J.J. Acuna acknowledges FONDECYT programs (projects no. 3140620 and 11160112).

Este estudio fue financiado por el proyecto cooperativo (código PCCI 1-2011) de la Comisión Nacional de Investigación Científica y Tecnológica (CONICYT, Chile) y el DAAD (Deutscher Akademischer Austauschdienst German). Este trabajo también fue financiado parcialmente con fondos de CONICYT-PCCI (código USA2013-0010) y el Fondo Nacional para el Desarrollo Científico y Tecnológico (FONDECYT, Chile, proyectos n.º 1120505 y 1160302). J.J. Acuña reconoce los programas FONDECYT (proyectos n.º 3140620 y 11160112).

10.1007/s00248-017-1057-0

pdf

Bacillus# Geobacillus# Geyser# Hot spring# Phytate# Thermophile

http://dx.doi.org/10.1007/s00248-017-1057-0

Ecology# Marine & Freshwater Biology# Microbiology

Bacilo# Geobacilo# Géiser# Fuente termal# fitato# termófilo

Biotecnología agrícola

Microbiología

Bioquímica

WOS:000418740200010

Screening and Characterization of Phytases from Bacteria Isolated from Chilean Hydrothermal Environments

Artículo original

version publicada

SPRINGER

MICROBIAL ECOLOGY

Ecología# Biología Marina y de Agua Dulce# Microbiología# microbiología

0095-3628

en

DAAD PCCI 1-2011#ANID PCCI 1-2011#ANID CONICYT-PCCI USA2013-0010#ANID FONDECYT 1120505#ANID FONDECYT 1160302#ANID FONDECYT 3140620#ANID FONDECYT 11160112

ANID CONICYT PCCI 1-2011

Deutscher Akademischer Austauschdienst German (DAAD, Germany)

ANID CONICYT PCCI USA2013-0010

ANID FONDECYT 1120505

ANID FONDECYT 1160302

ANID FONDECYT 3140620

ANID FONDECYT 11160112

This study was supported by cooperative project (code PCCI 1-2011) funded by The National Commission for Scientific and Technological Research (CONICYT, Chile) and Deutscher Akademischer Austauschdienst German (DAAD, Germany). This work was also partial financed with funds from CONICYT-PCCI (code USA2013-0010) and The National Fund for Scientific and Technological Development (FONDECYT, Chile, projects no. 1120505 and 1160302). J.J. Acuna acknowledges FONDECYT programs (projects no. 3140620 and 11160112).

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