Repositorio
Institucional

Arriagada, Cesar

Herrera, Hector#Valadares, Rafael#Oliveira, Guilherme#Fuentes, Alejandra#Almonacid, Leonardo#Vasconcelos do Nascimento, Sidney#Bashan, Yoav

Adaptation and tolerance mechanisms developed by mycorrhizal Bipinnula fimbriata plantlets (Orchidaceae) in a heavy metal-polluted ecosystem

SPRINGER

MYCORRHIZA

en

The adaptation and performance of orchid mycorrhizae in heavy metal-polluted soils have been poorly explored. In the present study, proteomic and metabolic approaches were used to detect physiological changes in orchid roots established in a heavy metal-polluted soil and to ascertain whether mycorrhizal fungi affect the metabolic responses of roots. Young Bipinnula fimbriata plantlets were established in control and heavy metal-polluted soils in a greenhouse. After 14 months, exudation of root organic acids, phenolics, percentage of mycorrhization, mineral content, and differential protein accumulation were measured. More root biomass, higher root colonization, and higher exudation rates of citrate, succinate, and malate were detected in roots growing in heavy metal-polluted soils. Higher accumulation of phosphorus and heavy metals was found inside mycorrhizal roots under metal stress. Under non-contaminated conditions, non-mycorrhizal root segments showed enhanced accumulation of proteins related to carbon metabolism and stress, whereas mycorrhizal root segments stimulated protein synthesis related to pathogen control, cytoskeleton modification, and sucrose metabolism. Under heavy metal stress, the proteome profile of non-mycorrhizal root segments indicates a lower induction of defense mechanisms, which, together with the stimulation of enzymes related to carotenoid biosynthesis and cell wall organization, may positively influence mycorrhizal fungi colonization. The results point to different metabolic strategies in mycorrhizal and non-mycorrhizal root segments that are exposed to heavy metal stress. The results indicate that root colonization by mycorrhizal fungi is stimulated to alleviate the negative effects of heavy metals in the orchids.

Artículo original

The authors thank the Instituto Tecnologico Vale (Belem, Brazil) for equipment supply and technical assistance and Ira Fogel for editorial and English improvement. This work was supported by the 'Fondo Nacional de Desarrollo Cientifico y Tecnologico' of Chile [grant number 1170931 to C.A.] and the 'Comision Nacional de Investigacion Cientifica y Tecnologica' of Chile [grant number 21130491 to H.H.].

Los autores agradecen al Instituto Tecnológico Vale (Belem, Brasil) por el suministro de equipos y la asistencia técnica, y a Ira Fogel por la mejora editorial y del inglés. Este trabajo contó con el apoyo del Fondo Nacional de Desarrollo Científico y Tecnológico de Chile [subvención número 1170931 a C.A.] y de la Comisión Nacional de Investigación Científica y Tecnológica de Chile [subvención número 21130491 a H.H.].

10.1007/s00572-018-0858-4

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Orchid mycorrhiza# Organic acid exudation# Proteome# Contaminated soil

http://dx.doi.org/10.1007/s00572-018-0858-4

Plant Sciences# Mycology

micorrizas de orquídeas# Exudación de ácidos orgánicos# proteoma# Suelo contaminado

Micología

Biología Molecular

Ciencias del Medio Ambiente

version publicada

hibrida

restringido

restringido

WOS:000447241100006

Adaptation and tolerance mechanisms developed by mycorrhizal <i>Bipinnula fimbriata </i>plantlets (Orchidaceae) in a heavy metal-polluted ecosystem

Artículo original

SPRINGER

MYCORRHIZA

Ciencias Vegetales# Micología

0940-6360

en

ANID FONDECYT 1170931#ANID 21130491

ANID FONDECYT 1170931

ANID CONICYT 21130491

The authors thank the Instituto Tecnologico Vale (Belem, Brazil) for equipment supply and technical assistance and Ira Fogel for editorial and English improvement. This work was supported by the 'Fondo Nacional de Desarrollo Cientifico y Tecnologico' of Chile [grant number 1170931 to C.A.] and the 'Comision Nacional de Investigacion Cientifica y Tecnologica' of Chile [grant number 21130491 to H.H.].

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