Repositorio
Institucional

Barrientos, Leticia

Lamina, Claudio#Braga, Douglas#Castro, Rui#Guimaraes, Carolina#de Castilho, Livia V. A.#Freire, Denise M. G.

⁢Streptomyces luridus⁢ So3.2 from Antarctic soil as a novel producer of compounds with bioemulsification potential

PUBLIC LIBRARY SCIENCE

PLOS ONE

en

The present study aimed to identify novel microbial producers of bioemulsificant compounds from Antarctic soils. Fifty-nine microbial strains were isolated from five different locations at South Shetland Islands, Antarctica, and screened for biosurfactant production by beta-hemolytic activity. Strain So 3.2 was determined as bioemulsifier-producer and identified by phenotypic and molecular characterization as Streptomyces luridus. Emulsification activity, oil displacement method and drop-collapsing test were performed to evaluate the biosurfactant activity with different oils and hydrocarbons using two different culture media (Luria Bertani and Bushnell Haas in the presence of different carbon sources: glucose, glycerol, olive oil and n-Hexadecane). Cell free supernatant of Bushnell Haas culture supplemented with n-Hexadecane showed the best results for all tests. Emulsification of hydrocarbons exceeded 60%, reaching up to 90% on oil with high API grade, while displacement tests ranged from 8 cm to 4 cm in diameter according the culture media and tested oils. Our results revealed that Streptomyces luridus So3.2 is able to produce bioemulsifiers capable of emulsifying hydrocarbons and oils, which could be used in different biotechnological applications, particularly for bioremediation of environments contaminated by oil leaks.

Artículo original

This work was supported by the Instituto Antartico Chileno, Ministerio de Relaciones Exteriores de Chile (http://www.inach.cl/inach/) Grants INACH DT_05-15 CL and Grant INACH RT_14-12 to LB and had a role in Data collection and logistical support. This was also funded by Universidad de La Frontera/Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro FAPERJ, (http://www.ufro.cl/) Grant FPJ15-0009 to LB and provided data analysis, Universidade Federal do Rio de Janeiro/ Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro FAPERJ (https://ufrj.br) D.F, R.C., C.A., L.A and provided data analysis, Direccion de Investigacion Universidad de La Frontera (http://www.ufro.cl/) Grant DI17-0116 to LB and provided data analysis and the Consejo Nacional de Ciencia y Tecnologia CONICYT (http://www.conicyt.cl/becasconicyt/2014/03/11/doctorado-en-chile-2014/) Grant No 21140691 to CL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript., We thank Ms. Judith Hoffman (Northern Light Translations & Editing) for her revision of the English manuscript. The authors gratefully acknowledge Grants INACH DT_05-15 and RT_14-12 from the Instituto Antartico Chileno, Ministerio de Relaciones Exteriores de Chile, Grant FPJ15-0009 from Universidad de La Frontera/Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro FAPERJ, and Grant DI17-0116 from Universidad de La Frontera -for logistical support. Claudio Lamilla is grateful for Conicyt Grant No 21140691.

Este trabajo fue apoyado por el Instituto Antártico Chileno, Ministerio de Relaciones Exteriores de Chile (http://www.inach.cl/inach/) Becas INACH DT_05-15 CL y Beca INACH RT_14-12 a LB y tuvo un rol en la recolección de datos y apoyo logístico. Esto también fue financiado por la Universidad de La Frontera/Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro FAPERJ, (http://www.ufro.cl/) Subvención FPJ15-0009 a LB y proporcionó análisis de datos, Universidade Federal do Rio de Janeiro/Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro FAPERJ (https://ufrj.br) D.F, R.C., C.A., L.A y proporcionó análisis de datos, Dirección de Investigación Universidad de La Frontera (http://www.ufro.cl/) Beca DI17-0116 a LB y proporcionó análisis de datos y el Consejo Nacional de Ciencia y Tecnología CONICYT (http://www.conicyt.cl/becasconicyt/2014/03/11/doctorado-en-chile-2014/) Beca No 21140691 a CL. Los financiadores no participaron en el diseño del estudio, la recopilación y el análisis de datos, la decisión de publicación ni la preparación del manuscrito. Agradecemos a la Sra. Judith Hoffman (Northern Light Translations & Editing) por su revisión del manuscrito en inglés. Los autores agradecen las subvenciones INACH DT_05-15 y RT_14-12 del Instituto Antártico Chileno, Ministerio de Relaciones Exteriores de Chile, la subvención FPJ15-0009 de la Universidad de La Frontera/Fundação de Amparo a Pesquisa do Estado do Rio de Janeiro FAPERJ, y la subvención DI17-0116 de la Universidad de La Frontera por el apoyo logístico. Claudio Lamilla agradece la subvención Conicyt n.º 21140691.

10.1371/journal.pone.0196054

pdf

http://dx.doi.org/10.1371/journal.pone.0196054

Multidisciplinary Sciences

Biorremediación

Biotecnología Ambiental

Microbiología

WOS:000430660600035

⁢Streptomyces luridus⁢ So3.2 from Antarctic soil as a novel producer of compounds with bioemulsification potential

Artículo original

version publicada

PUBLIC LIBRARY SCIENCE

PLOS ONE

Ciencias Multidisciplinarias

1932-6203

en

INACH DT_05-15#INACH RT_14-12#FAPERJ FPJ15-000#UFRO DI17-0116#ANID 21140691#UFRO FPJ15-0009

INACH DT_05-15 CL

INACH RT_14-12

UFRO FAPERJ FPJ15-0009

UFRO DI17-0116

ANID CONICYT 21140691

This work was supported by the Instituto Antartico Chileno, Ministerio de Relaciones Exteriores de Chile (http://www.inach.cl/inach/) Grants INACH DT_05-15 CL and Grant INACH RT_14-12 to LB and had a role in Data collection and logistical support. This was also funded by Universidad de La Frontera/Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro FAPERJ, (http://www.ufro.cl/) Grant FPJ15-0009 to LB and provided data analysis, Universidade Federal do Rio de Janeiro/ Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro FAPERJ (https://ufrj.br) D.F, R.C., C.A., L.A and provided data analysis, Direccion de Investigacion Universidad de La Frontera (http://www.ufro.cl/) Grant DI17-0116 to LB and provided data analysis and the Consejo Nacional de Ciencia y Tecnologia CONICYT (http://www.conicyt.cl/becasconicyt/2014/03/11/doctorado-en-chile-2014/) Grant No 21140691 to CL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript., We thank Ms. Judith Hoffman (Northern Light Translations & Editing) for her revision of the English manuscript. The authors gratefully acknowledge Grants INACH DT_05-15 and RT_14-12 from the Instituto Antartico Chileno, Ministerio de Relaciones Exteriores de Chile, Grant FPJ15-0009 from Universidad de La Frontera/Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro FAPERJ, and Grant DI17-0116 from Universidad de La Frontera -for logistical support. Claudio Lamilla is grateful for Conicyt Grant No 21140691.

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