Repositorio
Institucional

Campos-Vargas, Reinaldo

Balic, Ivan#Vizoso, Paula#Nilo-Poyanco, Ricardo#Sanhueza, Dayan#Olmedo, Patricio#Sepulveda, Pablo#Arriagada, Cesar#Defilippi, Bruno G.#Meneses, Claudio

Transcriptome analysis during ripening of table grape berry cv. Thompson Seedless

PUBLIC LIBRARY SCIENCE

PLOS ONE

en

Ripening is one of the key processes associated with the development of major organoleptic characteristics of the fruit. This process has been extensively characterized in climacteric fruit, in contrast with non-climacteric fruit such as grape, where the process is less understood. With the aim of studying changes in gene expression during ripening of non-climacteric fruit, an Illumina based RNA-Seq transcriptome analysis was performed on four developmental stages, between veraison and harvest, on table grapes berries cv Thompson Seedless. Functional analysis showed a transcriptional increase in genes related with degradation processes of chlorophyll, lipids, macromolecules recycling and nucleosomes organization, accompanied by a decrease in genes related with chloroplasts integrity and amino acid synthesis pathways. It was possible to identify several processes described during leaf senescence, particularly close to harvest. Before this point, the results suggest a high transcriptional activity associated with the regulation of gene expression, cytoskeletal organization and cell wall metabolism, which can be related to growth of berries and firmness loss characteristic to this stage of development. This high metabolic activity could be associated with an increase in the transcription of genes related with glycolysis and respiration, unexpected for a non-climacteric fruit ripening.

Artículo original

This work was supported by projects Fondecyt 1150492 (RC) and Fondecyt 3150538 (16). Both authors contributed in the conceptualization, formulations, leadership and preparation of the manuscript of this work.

Este trabajo fue financiado por los proyectos Fondecyt 1150492 (RC) y Fondecyt 3150538 (16). Ambos autores contribuyeron a la conceptualización, formulación, dirección y preparación del manuscrito de este trabajo.

10.1371/journal.pone.0190087

pdf

http://dx.doi.org/10.1371/journal.pone.0190087

Multidisciplinary Sciences

Viticulura

Biología Molecular

Botánica

WOS:000419689600031

Transcriptome analysis during ripening of table grape berry cv. Thompson Seedless

Artículo original

version publicada

PUBLIC LIBRARY SCIENCE

PLOS ONE

Ciencias Multidisciplinarias

1932-6203

en

ANID FONDECYT 150492#ANID FONDECYT 3150538

ANID FONDECYT 1150492

ANID FONDECYT 3150538

This work was supported by projects Fondecyt 1150492 (RC) and Fondecyt 3150538 (16). Both authors contributed in the conceptualization, formulations, leadership and preparation of the manuscript of this work.

pdf

dorada#verde

acceso abierto

acceso abierto

CC BY 4.0