Repositorio UFRO · Omeka S

Transcriptome analysis during ripening of table grape berry cv. Thompson Seedless

Primer Autor	Campos-Vargas, Reinaldo
Co-autores	Balic, Ivan#Vizoso, Paula#Nilo-Poyanco, Ricardo#Sanhueza, Dayan#Olmedo, Patricio#Sepulveda, Pablo#Arriagada, Cesar#Defilippi, Bruno G.#Meneses, Claudio
Título	Transcriptome analysis during ripening of table grape berry cv. Thompson Seedless
Editorial	PUBLIC LIBRARY SCIENCE
Revista	PLOS ONE
Lenguaje	en
Resumen	Ripening is one of the key processes associated with the development of major organoleptic characteristics of the fruit. This process has been extensively characterized in climacteric fruit, in contrast with non-climacteric fruit such as grape, where the process is less understood. With the aim of studying changes in gene expression during ripening of non-climacteric fruit, an Illumina based RNA-Seq transcriptome analysis was performed on four developmental stages, between veraison and harvest, on table grapes berries cv Thompson Seedless. Functional analysis showed a transcriptional increase in genes related with degradation processes of chlorophyll, lipids, macromolecules recycling and nucleosomes organization, accompanied by a decrease in genes related with chloroplasts integrity and amino acid synthesis pathways. It was possible to identify several processes described during leaf senescence, particularly close to harvest. Before this point, the results suggest a high transcriptional activity associated with the regulation of gene expression, cytoskeletal organization and cell wall metabolism, which can be related to growth of berries and firmness loss characteristic to this stage of development. This high metabolic activity could be associated with an increase in the transcription of genes related with glycolysis and respiration, unexpected for a non-climacteric fruit ripening.
Tipo de Recurso	Artículo original
Description	This work was supported by projects Fondecyt 1150492 (RC) and Fondecyt 3150538 (16). Both authors contributed in the conceptualization, formulations, leadership and preparation of the manuscript of this work. Este trabajo fue financiado por los proyectos Fondecyt 1150492 (RC) y Fondecyt 3150538 (16). Ambos autores contribuyeron a la conceptualización, formulación, dirección y preparación del manuscrito de este trabajo.
doi	10.1371/journal.pone.0190087
Formato Recurso	pdf
Ubicación del archivo	http://dx.doi.org/10.1371/journal.pone.0190087
Categoría OCDE	Multidisciplinary Sciences
Disciplinas de la OCDE	Viticulura Biología Molecular Botánica
Id de Web of Science	WOS:000419689600031
Título de la cita (Recomendado-único)	Transcriptome analysis during ripening of table grape berry cv. Thompson Seedless
Identificador del recurso (Mandatado-único)	Artículo original
Versión del recurso (Recomendado-único)	version publicada
Editorial	PUBLIC LIBRARY SCIENCE
Revista/Libro	PLOS ONE
Categoría WOS	Ciencias Multidisciplinarias
ISSN	1932-6203
Idioma	en
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID FONDECYT 150492#ANID FONDECYT 3150538 ANID FONDECYT 1150492 ANID FONDECYT 3150538
Descripción	This work was supported by projects Fondecyt 1150492 (RC) and Fondecyt 3150538 (16). Both authors contributed in the conceptualization, formulations, leadership and preparation of the manuscript of this work.
Formato	pdf
Tipo de ruta	dorada#verde
Access Rights	acceso abierto
Derechos de acceso	acceso abierto
License	CC BY 4.0