Repositorio
Institucional

de la Luz Mora, Maria

Parra-Almuna, Leyla#Diaz-Cortez, Andrea#Ferrol, Nuria

Aluminium toxicity and phosphate deficiency activates antioxidant systems and up-regulates expression of phosphate transporters gene in ryegrass (Lolium perenne L.) plants

ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER

PLANT PHYSIOLOGY AND BIOCHEMISTRY

en

Soil acidity, associated with aluminium (Al) toxicity and low phosphorus (P) availability, is considered the most important problem for agricultural production. Even though the Al-P interaction has been widely investigated, the impact of P-nutrition on Al-toxicity still remains controversial and poorly understood. To elucidate further insights into the underlying mechanisms of this interaction in ryegrass (Lolium perenne L.), P uptake, antioxidant responses and the gene expression of phosphate transporters were determined. Two ryegrass cultivars with different Al resistances, the Al-tolerant Nui cultivar and the Al-sensitive Expo cultivar were hydroponically grown under low (16 mu M) and optimal (100 mu M) P doses for 16 days. After P treatments, plants were exposed to Al doses (0 and 200 mu M) under acidic conditions (pH 4.8) for 24 h. Al and P accumulation were higher in the roots of Nui than that of Expo. Moreover, lower Al accumulation was found in shoots of Nui independent of P supplies. Oxidative stress induced by Al-toxicity and P-deficiency was more severe in the Al-sensitive Expo. Expression levels of L. perenne phosphate transporters were higher in Nui than they were in Expo. While LpPHT1 expression was up-regulated by P deficiency and Al toxicity in both cultivars, LpPHT4 expression only increased in the Al-tolerant cultivar. This report shows that the higher Al-tolerance of Nui can be attributed to a greater antioxidant system under both P conditions. The observation of higher P and Al accumulation in roots of Nui might indicate that the Al-tolerance of Nui is a consequence of Al immobilization by P mediated by the high expression of phosphate transporters.

Artículo original

This work was supported by the FONDECYT projects 1181050 and 1141247, the CONICYT Doctoral scholarship 21151320 and the Spanish Ministry of Economy, Industry and Competitivity (Project AGL2015-67098-R). We would like to thank the Scientific and Technological Bioresource Nucleus of Universidad de La Frontera (BIOREN-UFRO) for providing access to specialized equipment.

10.1016/j.plaphy.2018.07.031

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Aluminium toxicity# Phosphate deficiency# Phosphate transporter genes# Radical scavenging activity# Antioxidant enzymes

Aluminium toxicity# Phosphate deficiency# Phosphate transporter genes# Radical scavenging activity# Antioxidant enzymes

http://dx.doi.org/10.1016/j.plaphy.2018.07.031

Plant Sciences

Toxicidad del aluminio# Deficiencia de fosfato# Genes transportadores de fosfato# Actividad de captación radical# Enzimas antioxidantes

Toxicidad del aluminio# Deficiencia de fosfato# Genes transportadores de fosfato# Actividad de captación radical# Enzimas antioxidantes

WOS:000444789200043

Aluminium toxicity and phosphate deficiency activates antioxidant systems and up-regulates expression of phosphate transporters gene in ryegrass (<i>Lolium perenne</i> L.) plants

Artículo original

version publicada

ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER

PLANT PHYSIOLOGY AND BIOCHEMISTRY

Ciencias de las plantas

0981-9428

en

ANID FONDECYT 1181050#ANID FONDECYT 1141247#ANID CONICYT Doctora 21151320#MINECO AGL2015-67098-R

This work was supported by the FONDECYT projects 1181050 and 1141247, the CONICYT Doctoral scholarship 21151320 and the Spanish Ministry of Economy, Industry and Competitivity (Project AGL2015-67098-R). We would like to thank the Scientific and Technological Bioresource Nucleus of Universidad de La Frontera (BIOREN-UFRO) for providing access to specialized equipment.

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