Stomata regulation by tissue-specific expression of the Citrus sinensis MYB61 transcription factor improves water-use efficiency in Arabidopsis

Primer Autor
Arce-Johnson, Patricio
Co-autores
Romero-Romero, Jesus L.#Inostroza-Blancheteau, Claudio#Orellana, Daniela#Aquea, Felipe#Reyes-Diaz, Marjorie#Gil, Pilar M.#Pablo Matte, Juan
Título
Stomata regulation by tissue-specific expression of the Citrus sinensis MYB61 transcription factor improves water-use efficiency in Arabidopsis
Editorial
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
Revista
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Lenguaje
en
Resumen
Water-use efficiency (WUE) is a quantitative measurement of biomass produced per volume of water transpired by a plant. WUE is an important physiological trait for drought response to mitigate the water deficiency. In this work, a cisgenic construction from Citrus sinensis was developed and its function in the improvement of WUE was evaluated in Arabidopsis. Sequences of the CsMYB61 coding region, a transcription factor implicated in the closure of stomata, together with a putative stomata-specific promoter from CsMYB.1.5, were identified and cloned. The protein encoded in the CsMYB61 locus harbors domains and motifs characteristic of MYB61 proteins. In addition, a 1.2 kb promoter region of the gene CsMYB15 (pCsMYB15) containing regulatory elements for expression in guard cells and in response to Abscisic Acid (ABA) and light was isolated. In Arabidopsis, pCsMYB15 directs the expression of the reporter gene GUS in stomata in the presence of light. In addition, transgenic lines expressing the CsMYB61 coding region under transcriptional control of pCsMYB15 have a normal phenotype under in vitro and greenhouse conditions. These transgenic lines exhibited a smaller opening of the stomata pore, lower stomatal conductance and respiration rate, enhanced sensitivity to exogenous ABA, and high drought stress tolerance. Our results indicate that stomata-specific expression of CsMYB61 enhances water use efficiency under drought conditions in Arabidospis.
Tipo de Recurso
Artículo original
Description
The authors thank Valeria M. Borjas Mendez for technical assistance. Jesus L. Romero-Romero was supported by PhD fellowship CONACYT (SC392465), COTEBAL: 1865 and CONICYT Doctoral Scholarship for foreigners (63130094). This work was supported by FONDECYT 1150220 to PAJ, CAPES FB-002-2014 to FA and CONICYT PAI 82140040 to JPM. We thank Alyssa Grube for assistance in language support.
Los autores agradecen a Valeria M. Borjas Méndez por su asistencia técnica. Jesús L. Romero-Romero recibió apoyo de la beca de doctorado CONACYT (SC392465), COTEBAL: 1865 y la Beca de Doctorado CONICYT para extranjeros (63130094). Este trabajo recibió apoyo de FONDECYT 1150220 para PAJ, CAPES FB-002-2014 para FA y CONICYT PAI 82140040 para JPM. Agradecemos a Alyssa Grube por su apoyo lingüístico.
doi
10.1016/j.plaphy.2018.06.034
Formato Recurso
pdf
Palabras Claves
Drought tolerance# Abiotic stress# Water use efficiency# Arabidopsis# Cisgenic# MYB61 transcription factor# Citrus
Ubicación del archivo
http://dx.doi.org/10.1016/j.plaphy.2018.06.034
Categoría OCDE
Plant Sciences
Materias
Tolerancia a la sequía# Estrés abiótico# Eficiencia en el uso del agua# Arabidopsis# Cisgénico# factor de transcripción MYB61# Agrios
Disciplinas de la OCDE
Biotecnología agrícola
Tecnología de modificación genética
Genética y Herencia
Id de Web of Science
WOS:000444789200006
Título de la cita (Recomendado-único)
Stomata regulation by tissue-specific expression of the <i>Citrus sinensis</i> MYB61 transcription factor improves water-use efficiency in Arabidopsis
Identificador del recurso (Mandatado-único)
Artículo original
Versión del recurso (Recomendado-único)
version publicada
Editorial
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
Revista/Libro
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Categoría WOS
Ciencias de las plantas
ISSN
0981-9428
Idioma
en
Referencia del Financiador (Mandatado si es aplicable-repetible)
CONACYT SC392465#COTEBAL 1865#ANIDCONICYT Doctorado 63130094#ANID FONDECYT 1150220#CAPES FB-002-2014#ANID CONICYT PAI 82140040
CONACYT PHd SC392465
COTEBAL 1865
ANID CONICYT Doctoral Scholarship 63130094
ANID FONDECYT 1150220
CAPES FB-002-2014
ANID CONICYT PAI 82140040
Descripción
The authors thank Valeria M. Borjas Mendez for technical assistance. Jesus L. Romero-Romero was supported by PhD fellowship CONACYT (SC392465), COTEBAL: 1865 and CONICYT Doctoral Scholarship for foreigners (63130094). This work was supported by FONDECYT 1150220 to PAJ, CAPES FB-002-2014 to FA and CONICYT PAI 82140040 to JPM. We thank Alyssa Grube for assistance in language support.
Formato
pdf
Tipo de ruta
hibrida#verde
Access Rights
metadata
Derechos de acceso
metadata
Página de inicio (Recomendado-único)
97
Página final (Recomendado-único)
105
Colecciones
Colección Publicaciones Científicas
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