Repositorio
Institucional

Garces, M.

Ulloa, M.#Miranda, A.#Bravo, L. A.

Physiological and ultrastructural characterisation of a desiccation-tolerant filmy fern, Hymenophyllum caudiculatum: Influence of translational regulation and ABA on recovery

WILEY

PLANT BIOLOGY

en

The filmy fern Hymenophyllum caudiculatum can lose 60% of its relative water content, remain dry for some time and recover 88% of photochemical efficiency after 30min of rehydration. Little is known about the protective strategies and regulation of the cellular rehydration process in this filmy fern species. The aim of this study was to characterise the filmy fern ultrastructure during a desiccation-rehydration cycle, and measure the physiological effects of transcription/translation inhibitors and ABA during desiccation recovery. Confocal and transmission electron microscopy were used to compare changes in structure during fast or slow desiccation. Transcription (actinomycin D) and translation (cycloheximide) inhibitors and ABA were used to compare photochemical efficiency during desiccation recovery. Cell structure was conserved during slow desiccation and rehydration, constitutive properties of the cell wall, allowing invagination and folding of the membranes and an important change in chloroplast size. The use of a translational inhibitor impeded recovery of photochemical efficiency during the first 80min of rehydration, but the transcriptional inhibitor had no effect. Exogenous ABA delayed photochemical inactivation, and endogenous ABA levels decreased during desiccation and rehydration. Frond curling and chloroplast movements are possible strategies to avoid photodamage. Constitutive membrane plasticity and rapid cellular repair can be adaptations evolved to tolerate a rapid recovery during rehydration. Further research is required to explore the importance of existing mRNAs during the first minutes of recovery, and ABA function during desiccation of H.caudiculatum.

Artículo original

This research was supported by a grant from FONDECYT Iniciacion 11130717 and Direccion de Cooperacion Internacional, Universidad de La Frontera. Melvin Oliver is gratefully acknowledged for analysis of endogenous ABA. The authors thank Katalapi park (http://www.parquekatalapi.cl) for providing logistic support and permission to collect plant material in the park.

Esta investigación fue financiada con una beca del FONDECYT Iniciación 11130717 y la Dirección de Cooperación Internacional de la Universidad de La Frontera. Se agradece a Melvin Oliver el análisis del ABA endógeno. Los autores agradecen al parque Katalapi (http://www.parquekatalapi.cl) por el apoyo logístico y el permiso para recolectar material vegetal en el parque.

10.1111/plb.12660

pdf

Drought stress# filmy fern# translational regulation

http://dx.doi.org/10.1111/plb.12660

Plant Sciences

Estrés por sequía# helecho vaporoso# regulación traslacional

Botánica

Biología Celular

Bioquímica

WOS:000427259500014

Physiological and ultrastructural characterisation of a desiccation-tolerant filmy fern, <i>Hymenophyllum caudiculatum</i>: Influence of translational regulation and ABA on recovery

Artículo original

version publicada

WILEY

PLANT BIOLOGY

Ciencias de las plantas

1435-8603

en

ANID FONDECYT INICIACIÓN 11130717#UFRO

ANID FONDECYT Iniciacion 11130717

UFRO Direccion de Cooperacion Internacional

This research was supported by a grant from FONDECYT Iniciacion 11130717 and Direccion de Cooperacion Internacional, Universidad de La Frontera. Melvin Oliver is gratefully acknowledged for analysis of endogenous ABA. The authors thank Katalapi park (http://www.parquekatalapi.cl) for providing logistic support and permission to collect plant material in the park.

pdf

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