Repositorio
Institucional

Rodrigues Marques, Marcelo

Cerda, Alvaro#Fontanari, Gustavo Guadagnucci#Pimenta, Daniel Carvalho#Soares-Freitas, Rosana Manolio#Hirata, Mario Hiroyuki#Crespo Hirata, Rosario Dominguez#Gomes Areas, Jose Alfredo

Transport of cowpea bean derived peptides and their modulator effects on mRNA expression of cholesterol-related genes in Caco-2 and HepG2 cells

ELSEVIER SCIENCE BV

FOOD RESEARCH INTERNATIONAL

en

"This work studied the cell transport of peptidase-generated peptides from cowpea bean proteins and their effects on mRNA expression of cholesterol-related genes in intestinal and liver cells. The <= 3 kDa hydrolysate was obtained and incubated with Caco-2 intestinal cells using Transwell center dot plates. HepG2 liver cells were incubated with synthetic analogues of peptides (MELNAVSVVHS and MELNAVSVVSH) identified by ""de novo"" peptide sequencing in the Caco-2 monolayer permeates. The mRNA expression of NPC1L1, ABCA1 and ABCG1 was measured in Caco-2 cells, in the presence or absence of <= 3 kDa hydrolysate and the expression of HMGCR, SREBP2, LXRa, AMPK1, was determined in the HepG2 cells in the presence or absence of synthetic peptides. Exposure of Caco-2 cells to cowpea <= 3 kDa hydrolysate (2.5 and 5 mg/mL) increased ABCG1 expression at 6 h and 12 h. SREBP2, HMGCR and LDLR mRNA levels were reduced in HepG2 cells after 24 h of treatment with MELNAVSVVHS peptide (50 mu M and 100 mu M). These results suggest that MELNAVSVVHS peptide is able to cross intestinal barrier and to modulate genes involved in cholesterol homeostasis."

Artículo original

The author MR Marques is grateful to FAPESP (Foundation for research support of the State of Sao Paulo, Brazil) PhD scholarship 2013/09304-2 and research grant 2012/15900-4. The authors and sponsor declare no conflict of interest. We wish to thank Amanda Caroline C. C. Carlos for collaboration on hydrolysis.

El autor, Sr. Marques, agradece la beca de doctorado 2013/09304-2 y la beca de investigación 2012/15900-4 de la FAPESP (Fundación de Apoyo a la Investigación del Estado de São Paulo, Brasil). Los autores y el patrocinador declaran no tener ningún conflicto de intereses. Agradecemos a Amanda Caroline C. C. Carlos por su colaboración en el área de hidrólisis.

10.1016/j.foodres.2018.01.031

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Cowpea bean# Hydrolysate# Peptides uptake# Cholesterol# Intestinal barrier

http://dx.doi.org/10.1016/j.foodres.2018.01.031

Food Science & Technology

frijol caupí# hidrolizado# Captación de péptidos# Colesterol# Barrera intestinal

Biotecnología Alimentaria

Biología Molecular

Nutrición y Dietética

WOS:000430770700018

Transport of cowpea bean derived peptides and their modulator effects on mRNA expression of cholesterol-related genes in Caco-2 and HepG2 cells

Artículo original

version publicada

ELSEVIER SCIENCE BV

FOOD RESEARCH INTERNATIONAL

Ciencia y tecnología de los alimentos

0963-9969

en

FAPESP 2013/09304-2#FAPESP 2012/15900-4

FAPESP 2013/09304-2

FAPESP 2012/15900-4

The author MR Marques is grateful to FAPESP (Foundation for research support of the State of Sao Paulo, Brazil) PhD scholarship 2013/09304-2 and research grant 2012/15900-4. The authors and sponsor declare no conflict of interest. We wish to thank Amanda Caroline C. C. Carlos for collaboration on hydrolysis.

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hibrida#verde

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