Effects of Diet-Induced Obesity and Deficient in Vitamin D on Spermatozoa Function and DNA Integrity in Sprague-Dawley Rats
| Primer Autor |
Risopatron, J.
|
| Co-autores |
Merino, O.#Sanchez, R.#Gregorio, B. M.#Sampaio, F. J.
|
| Título |
Effects of Diet-Induced Obesity and Deficient in Vitamin D on Spermatozoa Function and DNA Integrity in Sprague-Dawley Rats
|
| Editorial |
HINDAWI LTD
|
| Revista |
BIOMED RESEARCH INTERNATIONAL
|
| Lenguaje |
en
|
| Resumen |
Obesity has adverse effects on male fertility and usually is diagnosed with a prevalence of vitamin D deficiency (VD-). Discussion on the impact of obesity/VD- on sperm function has been limited. This study analyzed the effects of diet-induced obesity/VD- on viability and plasmamembrane integrity (PMI), superoxide anion (O-2(-)) level, and DNA fragmentation (DNA(frag)) in sperm Sprague-Dawley rats. The males were randomized into four groups and fed for a period of 12 weeks: G1: control diet with vitamin D(C/VD+), G2: control diet without vitamin D (C/VD-), G3: high-fat diet with vitamin D (HF/VD+), and G4: high-fat diet without vitamin D (HF/VD-). Sperm function parameters were analyzed by flow cytometry. PMI percentages and O-2(-) levels were not affected by any of the diets. DNA fragmentation was increasing significantly (p<0.05) in the spermatozoa of animals with diets vitamin D deficient (G2) and diet-induced obesity (G4). Our results allow us to point out that diet-induced obesity and VD- produce greater damage in DNA sperm of rats. The use of nutraceuticals containing vitamin D could be reducing the risk of fragmentation of DNA in spermatozoa.
|
| Tipo de Recurso |
Artículo original
|
| Description |
This work was supported by FAPERJ-UFRO No FPJ15-0014 and National Commission for Scientific and Technological Research (CONICYT-PFCHA/Doctorado Nacional/2015-21151103), Chile.
Este trabajo contó con el apoyo de FAPERJ-UFRO No FPJ15-0014 y la Comisión Nacional de Investigaciones Científicas y Tecnológicas (CONICYT-PFCHA/Doctorado Nacional/2015-21151103), Chile.
|
| doi |
10.1155/2018/5479057
|
| Formato Recurso |
pdf
|
| Ubicación del archivo |
http://dx.doi.org/10.1155/2018/5479057
|
| Categoría OCDE |
Biotechnology & Applied Microbiology# Medicine, Research & Experimental
|
| Disciplinas de la OCDE |
Andrología
Endocrinología y Metabolismo (Incluye Diabetes, Hormonas)
Fisiología
|
| Id de Web of Science |
WOS:000452966400001
|
| Título de la cita (Recomendado-único) |
Effects of Diet-Induced Obesity and Deficient in Vitamin D on Spermatozoa Function and DNA Integrity in Sprague-Dawley Rats
|
| Identificador del recurso (Mandatado-único) |
Artículo original
|
| Versión del recurso (Recomendado-único) |
version publicada
|
| Editorial |
HINDAWI LTD
|
| Revista/Libro |
BIOMED RESEARCH INTERNATIONAL
|
| Categoría WOS |
Biotecnología y Microbiología Aplicada# Medicina, Investigación y Experimental
|
| ISSN |
2314-6133
|
| Idioma |
en
|
| Referencia del Financiador (Mandatado si es aplicable-repetible) |
FAPERJ-UFRO FPJ15-0014#ANID CONICYT-PFCHA/Doctorado Nacional/2015 21151103
FAPERJ-UFRO FPJ15-0014
ANID CONICYT PFCHA 2015 21151103
|
| Descripción |
This work was supported by FAPERJ-UFRO No FPJ15-0014 and National Commission for Scientific and Technological Research (CONICYT-PFCHA/Doctorado Nacional/2015-21151103), Chile.
|
| Formato |
pdf
|
| Tipo de ruta |
dorada#verde
|
| Access Rights |
acceso abierto
|
| Derechos de acceso |
acceso abierto
|
| License |
CC BY
|
- Colecciones
- Colección Publicaciones Científicas
cienciaabierta.ufro.cl
ciencia.abierta@ufrontera.cl