Repositorio
Institucional

Concha, Eric

Heipieper, Hermann J.#Wick, Lukas Y.#Ciudad, Gustavo A.#Navia, Rodrigo

Effects of limonene, n-decane and n-decanol on growth and membrane fatty acid composition of the microalga Botryococcus braunii

SPRINGEROPEN

AMB EXPRESS

en

Botryococcus braunii is a promising microalga for the production of biofuels and other chemicals because of its high content of internal lipids and external hydrocarbons. However, due to the very thick cell wall of B. braunii, traditional chemical/physical downstream processing very often is not as effective as expected and requires high amounts of energy. In this cases, the application of two-phase aqueous-organic solvent systems could be an alternative to cultivate microalgae allowing for a simultaneous extraction of the valuable compounds without significant negative effects on cell growth. Two-phase systems have been applied before, however, there are no studies so far on the mechanisms used by microalgae to survive in contact with solvents present as a second-phase. In this study, the effects of the solvents limonene, n-decane and n-decanol on growth of the microalga B. braunii as well as the adaptive cell response in terms of their phospholipid fatty acid contents were analized. A concentration-dependent negative effect of all three solvents on cell growth was observed. Effects were accompanied by changes of the membrane fatty acid composition of the alga as manifested by a decrease of the unsaturation . In addition, an association was found between the solvent hydrophobicity (given as log octanol-water partition coefficient () values) and their toxic effects, whereby n-decanol and n-decane emerged as the most and least toxic solvent respectively. Among the tested solvents, the latter promises to be the most suitable for a two-phase extraction system.

Artículo original

This study was funded by CONICYT award numbers 781211006, 24121442, 75120043, Anillo de Investigacion en Ciencia y Tecnologia GAMBIO Project No. ACT172128, CONICYT and FONDECYT 1150707.Z

Este estudio fue financiado por los premios CONICYT números 781211006, 24121442, 75120043, Anillo de Investigación en Ciencia y Tecnología GAMBIO Proyecto No. ACT172128, CONICYT y FONDECYT 1150707.Z

10.1186/s13568-018-0718-9

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Botryococcus braunii# Two-phase system# Solvent tolerance# Fatty acid profile# Unsaturation index

http://dx.doi.org/10.1186/s13568-018-0718-9

Biotechnology & Applied Microbiology

Botryococcus braunii# Sistema bifásico# Tolerancia a los disolventes# Perfil de ácidos grasos# Índice de insaturación

Biotecnología Ambiental

Bioproductos (Productos que se manufacturan usando biotecnología como materia prima), Biomateriales, Bioplásticos, Biocombustibles, Químicos Brutos y Finos, Bioderivados, Materiales nuevos Bioderivados

Microbiología

WOS:000452568300001

Effects of limonene, <i>n</i>-decane and <i>n</i>-decanol on growth and membrane fatty acid composition of the microalga <i>Botryococcus braunii</i>

Artículo original

version publicada

SPRINGEROPEN

AMB EXPRESS

Biotecnología y Microbiología Aplicada

2191-0855

en

ANID CONICYT 781211006#ANID CONICYT 24121442#ANID CONICYT 75120043#ANID CONICYT ACT172128#ANID CONICYT FONDECYT 1150707.Z

ANID CONICYT 781211006

ANID CONICYT 24121442

ANID CONICYT 75120043

ANID ACT172128

ANID FONDECYT 1150707

This study was funded by CONICYT award numbers 781211006, 24121442, 75120043, Anillo de Investigacion en Ciencia y Tecnologia GAMBIO Project No. ACT172128, CONICYT and FONDECYT 1150707.Z

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acceso abierto

CC BY 4.0

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