Repositorio
Institucional

Cuozzo, S. A.

Sineli, P. E.#Davila Costa, J.#Tortella, G.

Streptomyces sp is a powerful biotechnological tool for the biodegradation of HCH isomers: biochemical and molecular basis

TAYLOR & FRANCIS LTD

CRITICAL REVIEWS IN BIOTECHNOLOGY

en

Actinobacteria are well-known degraders of toxic materials that have the ability to tolerate and remove organochloride pesticides, thus, they are used for bioremediation. The biodegradation of organochlorines by actinobacteria has been demonstrated in pure and mixed cultures with the concomitant production of metabolic intermediates including -pentachlorocyclohexene (-PCCH), 1,3,4,6-tetrachloro-1,4-cyclohexadiene (1,4-TCDN), 1,2-dichlorobenzene (1,2-DCB), 1,3-dichlorobenzene (1,3-DCB), or 1,4-dichlorobenzene (1,4-DCB), 1,2,3-trichlorobenzene (1,2,3-TCB), 1,2,4-trichlorobenzene (1,2,4-TCB), or 1,3,5-trichlorobenzene (1,3,5-TCB), 1,3-DCB, and 1,2-DCB. Chromatography coupled to mass spectrometric detection, especially GC-MS, is typically used to determine HCH-isomer metabolites. The important enzymes involved in HCH isomer degradation metabolic pathways include hexachlorocyclohexane dehydrochlorinase (LinA), haloalkane dehalogenase (LinB), and alcohol dehydrogenase (LinC). The metabolic versatility of these enzymes is known. Advances have been made in the identification of actinobacterial haloalkane dehydrogenase, which is encoded by linB. This knowledge will permit future improvements in biodegradation processes using Actinobacteria. The enzymatic and genetic characterizations of the molecular mechanisms involved in these processes have not been fully elucidated, necessitating further studies. New advances in this area suggest promising results. The scope of this paper encompasses the following: (i) the aerobic degradation pathways of hexachlorocyclohexane (HCH) isomers, (ii) the important genes and enzymes involved in the metabolic pathways of HCH isomer degradation, and (iii) the identification and quantification of intermediate metabolites through gas chromatography coupled to mass spectrometry (GC-MS).

Artículo de revisión

10.1080/07388551.2017.1398133

pdf

Actinobacteria# bioremediation# degradation# HCH# isomers

http://dx.doi.org/10.1080/07388551.2017.1398133

Biotechnology & Applied Microbiology

actinobacterias# biorremediación# degradación# HCH# isómeros

Biorremediación

Biotecnología Ambiental

Microbiología

WOS:000433133800006

<i>Streptomyces</i> sp is a powerful biotechnological tool for the biodegradation of HCH isomers: biochemical and molecular basis

hdl.handle.net/11336/39570

Artículo de revisión

version publicada

TAYLOR & FRANCIS LTD

CRITICAL REVIEWS IN BIOTECHNOLOGY

Biotecnología y Microbiología Aplicada

0738-8551

en

pdf

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