Repositorio
Institucional

Hirabara, Sandro M.

Martins, Amanda R.#Crisma, Amanda R.#Masi, Laureane N.#Amaral, Catia L.#Marzuca-Nassr, Gabriel N.#Bomfim, Lucas H. M.#Teodoro, Bruno G.#Queiroz, Andre L.#Serdan, Tamires D. A.#Torres, Rosangela P.#Mancini-Filho, Jorge#Rodrigues, Alice C.#Alba-Loureiro, Tatiana C.#Pithon-Curi, Tania C.#Gorjao, Renata#Silveira, Leonardo R.#Curi, Rui#Newsholme, Philip

Attenuation of obesity and insulin resistance by fish oil supplementation is associated with improved skeletal muscle mitochondrial function in mice fed a high-fat diet

ELSEVIER SCIENCE INC

JOURNAL OF NUTRITIONAL BIOCHEMISTRY

en

Omega-3 polyunsaturated fatty acids (n-3 PUFAs) have been reported to improve insulin sensitivity and glucose homeostasis in animal models of insulin resistance, but the involved mechanisms still remain unresolved. In this study, we evaluated the effects of fish oil (FO), a source of n-3 PUFAs, on obesity, insulin resistance and muscle mitochondrial function in mice fed a high-fat diet (HFD). C57B1/6 male mice, 8 weeks old, were divided into four groups: control diet (C), high-fat diet (H), C+FO (CFO) and H+FO (HFO). FO was administered by oral gavage (2 g/kg b.w.), three times a week, starting 4 weeks before diet administration until the end of the experimental protocol. HFD-induced obesity and insulin resistance associated with impaired skeletal muscle mitochondrial function, as indicated by decreased oxygen consumption, tricarboxylic acid cycle intermediate (TCAi) contents (citrate, alpha-ketoglutarate, malate and oxaloacetate), oxidative phosphorylation protein content and mitochondrial biogenesis. These effects were associated with elevated reactive oxygen species production, decreased PGC1-a transcription and reduced Akt phosphorylation. The changes induced by the HFD were partially attenuated by FO, which decreased obesity and insulin resistance and increased mitochondria] function. In the H group, FO supplementation also improved oxygen consumption, increased TCAi content, and Akt and AMPK phosphorylation, and up-regulated mRNA expression of Gpat1, Pepck, catalase and mitochondrial proteins (Pgc1 alpha, Ppar alpha, Cpt1 and Ucp3). These results suggest that dietary FO attenuates the deleterious effects of the HFD (obesity and insulin resistance) by improving skeletal muscle mitochondrial function. (C) 2017 Elsevier Inc. All rights reserved.

Artículo original

FAPESP, CNPq, CAPES, and Guggenheim Foundation supported this study. PN received FAPESP travel support and is grateful to the Curtin University School of Biomedical Sciences for research support. Authors are grateful for the technical assistance from Dr. Gilson Masahiro Murata and Mr. Jose Roberto Mendonca.

Este estudio contó con el apoyo de la FAPESP, el CNPq, la CAPES y la Fundación Guggenheim. PN recibió apoyo de la FAPESP para viajes y agradece a la Facultad de Ciencias Biomédicas de la Universidad de Curtin por el apoyo a la investigación. Los autores agradecen la asistencia técnica del Dr. Gilson Masahiro Murata y del Sr. José Roberto Mendonca.

10.1016/j.jnutbio.2017.11.012

pdf

Insulin resistance# Fish oil# Skeletal muscle# Mitochondrial function# Obesity

http://dx.doi.org/10.1016/j.jnutbio.2017.11.012

Biochemistry & Molecular Biology# Nutrition & Dietetics

Resistencia a la insulina# Aceite de pescado# Músculo esquelético# Función mitocondrial# Obesidad

Nutrición y Dietética

Fisiología

Bioquímica

version publicada

hibrida

verde

metadata

metadata

WOS:000432416000008

Attenuation of obesity and insulin resistance by fish oil supplementation is associated with improved skeletal muscle mitochondrial function in mice fed a high-fat diet

Artículo original

ELSEVIER SCIENCE INC

JOURNAL OF NUTRITIONAL BIOCHEMISTRY

Bioquímica y Biología Molecular# Nutrición y Dietética

0955-2863

en

FAPESP#CNPq#CAPES

FAPESP

CNPq

CAPES

Guggenheim Foundation

Curtin University School of Biomedical Sciences

FAPESP, CNPq, CAPES, and Guggenheim Foundation supported this study. PN received FAPESP travel support and is grateful to the Curtin University School of Biomedical Sciences for research support. Authors are grateful for the technical assistance from Dr. Gilson Masahiro Murata and Mr. Jose Roberto Mendonca.

pdf

780

784