Repositorio
Institucional

Jorquera, Milko A.

Cid, Fernanda P.#Maruyama, Fumito#Murase, Kazunori#Graether, Steffen P.#Larama, Giovanni#Bravo, Leon A.

Draft genome sequences of bacteria isolated from the Deschampsia antarctica phyllosphere

SPRINGER JAPAN KK

EXTREMOPHILES

en

Genome analyses are being used to characterize plant growth-promoting (PGP) bacteria living in different plant compartiments. In this context, we have recently isolated bacteria from the phyllosphere of an Antarctic plant (Deschampsia antarctica) showing ice recrystallization inhibition (IRI), an activity related to the presence of antifreeze proteins (AFPs). In this study, the draft genomes of six phyllospheric bacteria showing IRI activity were sequenced and annotated according to their functional gene categories. Genome sizes ranged from 5.6 to 6.3 Mbp, and based on sequence analysis of the 16S rRNA genes, five strains were identified as Pseudomonas and one as Janthinobacterium. Interestingly, most strains showed genes associated with PGP traits, such as nutrient uptake (ammonia assimilation, nitrogen fixing, phosphatases, and organic acid production), bioactive metabolites (indole acetic acid and 1-aminocyclopropane-1-carboxylate deaminase), and antimicrobial compounds (hydrogen cyanide and pyoverdine). In relation with IRI activity, a search of putative AFPs using current bioinformatic tools was also carried out. Despite that genes associated with reported AFPs were not found in these genomes, genes connected to ice-nucleation proteins (InaA) were found in all Pseudomonas strains, but not in the Janthinobacterium strain.

Artículo original

This study was financed with funds from Chilean Antarctic Institute-INACH (code RT_02_16), The National Fund for Scientific and Technological Development-FONDECYT (No. 1160302) and The National Commission for Scientific and Technological Research-CONICYT (Code USA2013-0010 and MEC no. 80140015). This work was supported by the Ministry of Education, Culture, Sports, Science and Technology in Japan or Japan Society for the Promotion of Science under Grants-in-Aid for Scientific Research (KAKENHI) (Grant Numbers 16H05830/16H05501/16H01782/ 16H02767), Kurita Water and Environment Foundation Grant, Ichiro Kanehara Foundation Scholarship Grant for Research in Basic Medical Sciences and Medical Care, Senri Life Science Foundation Kishimoto Grant, and Japan Agency for Medical Research and Development (AMED), and also by National Science and Engineering Research Council of Canada (NSERC). F. Cid thanks to the doctor scholarships awarded by INACH (Code DT_01-13), CONICYT (No. 21140534), MECESUP FRO1204 and La Frontera University.

Este estudio fue financiado con fondos del Instituto Antártico Chileno-INACH (código RT_02_16), del Fondo Nacional de Desarrollo Científico y Tecnológico-FONDECYT (N° 1160302) y de la Comisión Nacional de Investigación Científica y Tecnológica-CONICYT (Código USA2013-0010 y MEC N° 80140015). Este trabajo fue financiado por el Ministerio de Educación, Cultura, Deportes, Ciencia y Tecnología de Japón o la Sociedad Japonesa para la Promoción de la Ciencia bajo subvenciones para la investigación científica (KAKENHI) (números de subvención 16H05830/16H05501/16H01782/16H02767), la subvención de la Fundación Kurita para el Agua y el Medio Ambiente, la subvención de la Fundación Ichiro Kanehara para la investigación en ciencias médicas básicas y atención médica, la subvención Kishimoto de la Fundación Senri para las Ciencias de la Vida y la Agencia Japonesa para la Investigación y el Desarrollo Médico (AMED), y también por el Consejo Nacional de Investigación en Ciencia e Ingeniería de Canadá (NSERC). F. Cid agradece las becas de doctorado otorgadas por INACH (Código DT_01-13), CONICYT (No. 21140534), MECESUP FRO1204 y la Universidad de La Frontera.

10.1007/s00792-018-1015-x

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Antarctic bacteria# Antifreeze proteins# Ice recrystallization inhibition# Deschampsia antarctica# Phyllosphere# Plant growth-promoting bacteria

http://dx.doi.org/10.1007/s00792-018-1015-x

Biochemistry & Molecular Biology# Microbiology

bacterias antárticas# Proteínas anticongelantes# Inhibición de la recristalización del hielo# Deschampsia antártida# filosfera# Bacterias promotoras del crecimiento de las plantas.

Biotecnología agrícola

Microbiología

Genética y Herencia

WOS:000428048800016

Draft genome sequences of bacteria isolated from the <i>Deschampsia antarctica</i> phyllosphere

Artículo original

version publicada

SPRINGER JAPAN KK

EXTREMOPHILES

Bioquímica y Biología Molecular# Microbiología

1431-0651

en

INACH RT 02 16#ANID FONDECYT 1160302#

INACH RT_02_16

ANID FONDECYT 1160302

ANID CONICYT USA2013-0010

ANID CONICYT MEC 80140015

Ministry of Education, Culture, Sports, Science and Technology in Japan

Japan Society for the Promotion of Science 16H05830

Japan Society for the Promotion of Science 16H05501

Japan Society for the Promotion of Science 16H01782

Japan Society for the Promotion of Science 16H02767]

Kurita Water and Environment Foundation

Ichiro Kanehara Foundation

Senri Life Science Foundation Kishimoto Grant

Japan Agency for Medical Research and Development (AMED)

National Science and Engineering Research Council, Canada (NSERC)

INACH DT_01-13

ANID CONICYT 21140534

UFRO MECESUP FRO1204

La Frontera University

Grants-in-Aid for Scientific Research [16H01782, 16H05830, 16H05501, 16H02767] Funding Source: KAKEN

This study was financed with funds from Chilean Antarctic Institute-INACH (code RT_02_16), The National Fund for Scientific and Technological Development-FONDECYT (No. 1160302) and The National Commission for Scientific and Technological Research-CONICYT (Code USA2013-0010 and MEC no. 80140015). This work was supported by the Ministry of Education, Culture, Sports, Science and Technology in Japan or Japan Society for the Promotion of Science under Grants-in-Aid for Scientific Research (KAKENHI) (Grant Numbers 16H05830/16H05501/16H01782/ 16H02767), Kurita Water and Environment Foundation Grant, Ichiro Kanehara Foundation Scholarship Grant for Research in Basic Medical Sciences and Medical Care, Senri Life Science Foundation Kishimoto Grant, and Japan Agency for Medical Research and Development (AMED), and also by National Science and Engineering Research Council of Canada (NSERC). F. Cid thanks to the doctor scholarships awarded by INACH (Code DT_01-13), CONICYT (No. 21140534), MECESUP FRO1204 and La Frontera University.

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