Analysis of the grapevine moth Lobesia botrana antennal transcriptome and it expression of odorant-binding and chemosensory proteins

Primer Autor
Venthur, Herbert
Co-autores
Rojas, Valentina#Jimenez, Hector#Palma-Millanao, Ruben#Gonzalez-Gonzalez, Angelica#Machuca, Juan#Godoy, Ricardo#Ceballos, Ricardo#Mutis, Ana
Título
Analysis of the grapevine moth Lobesia botrana antennal transcriptome and it expression of odorant-binding and chemosensory proteins
Editorial
ELSEVIER SCIENCE INC
Revista
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
Lenguaje
en
Resumen
The grapevine moth, Lobesia botrana, is considered a harmful pest for vineyards in Chile as well as in North America and Europe. Currently, monitoring and control methods of L. botrana are based on its main sex pheromone component, being effective for low population densities. In order to improve control methods, antennal olfactory proteins in moths, such as odorant-binding proteins (OBPs) and odorant receptors (ORs) have been studied as promising targets for the discovery of new potent semiochemicals, which have not been reported for L. botrana. Therefore, the objective of this study was to identify the repertoire of proteins related to chemoreception in L. botrana by antennal transcriptome and analyze the relative expression of OBPs and CSPs in male and female antennae. Through next-generation sequencing of the antennal transcriptome by Ilumina HiSeq2500 we identified a total of 118 chemoreceptors, from which 61, 42 and 15 transcripts are related to ORs, ionotropic receptors (IRs) and gustatory receptors (GRs), respectively. Furthermore, RNA-Seq data revealed 35 transcripts for OBPs and 18 for chemosensory proteins (CSPs). Analysis by qRT-PCR showed 20 OBPs significantly expressed in female antennae, while 5 were more expressed in males. Similarly, most of the CSPs were significantly expressed in female than male antennae. All the olfactory-related sequences were compared with homologs and their phylogenetic relationships elucidated. Finally, our findings in relation to the improvement of L. botrana management are discussed.
Tipo de Recurso
Artículo original
Description
The authors would like to thank Dr. Ricardo Ceballos from Instituto de Investigaciones Agropecuarias (INIA), Quilamapu, Laboratorio de Ecologia Quimica, Chillan, the financial support of INIA's Project 502185-70 and INIA La Cruz for the insects supply. This study was supported by Fondecyt de Iniciacion 11140668 and DIUFRO DFP16-0020.
doi
10.1016/j.cbd.2018.04.003
Formato Recurso
pdf
Palabras Claves
Lobesia botrana# Chemosensory receptors# Soluble proteins# Relative expression# Antennae
Lobesia botrana# Chemosensory receptors# Soluble proteins# Relative expression# Antennae
Ubicación del archivo
http://dx.doi.org/10.1016/j.cbd.2018.04.003
Categoría OCDE
Biochemistry & Molecular Biology# Genetics & Heredity
Materias
Lobesia botrana# Receptores quimiosensoriales# Proteínas solubles# Expresión relativa# Antenas
Lobesia botrana# Receptores quimiosensoriales# Proteínas solubles# Expresión relativa# Antenas
Id de Web of Science
WOS:000442710500001
Título de la cita (Recomendado-único)
Analysis of the grapevine moth <i>Lobesia botrana</i> antennal transcriptome and it expression of odorant-binding and chemosensory proteins
Identificador del recurso (Mandatado-único)
Artículo original
Versión del recurso (Recomendado-único)
version publicada
Editorial
ELSEVIER SCIENCE INC
Revista/Libro
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
Categoría WOS
Bioquímica y Biología Molecular# Genética y herencia
ISSN
1744-117X
Idioma
en
Referencia del Financiador (Mandatado si es aplicable-repetible)
INIA 502185-70#UFRO DFP16-0020#ANID FONDECYT 11140668
Descripción
The authors would like to thank Dr. Ricardo Ceballos from Instituto de Investigaciones Agropecuarias (INIA), Quilamapu, Laboratorio de Ecologia Quimica, Chillan, the financial support of INIA's Project 502185-70 and INIA La Cruz for the insects supply. This study was supported by Fondecyt de Iniciacion 11140668 and DIUFRO DFP16-0020.
Formato
pdf
Tipo de ruta
hibrida#verde
Access Rights
metadata
Derechos de acceso
metadata
Página de inicio (Recomendado-único)
26
Página final (Recomendado-único)
30
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