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Institucional

Venthur, Herbert

Rojas, Valentina#Jimenez, Hector#Palma-Millanao, Ruben#Gonzalez-Gonzalez, Angelica#Machuca, Juan#Godoy, Ricardo#Ceballos, Ricardo#Mutis, Ana

Analysis of the grapevine moth Lobesia botrana antennal transcriptome and it expression of odorant-binding and chemosensory proteins

ELSEVIER SCIENCE INC

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS

en

The grapevine moth, Lobesia botrana, is considered a harmful pest for vineyards in Chile as well as in North America and Europe. Currently, monitoring and control methods of L. botrana are based on its main sex pheromone component, being effective for low population densities. In order to improve control methods, antennal olfactory proteins in moths, such as odorant-binding proteins (OBPs) and odorant receptors (ORs) have been studied as promising targets for the discovery of new potent semiochemicals, which have not been reported for L. botrana. Therefore, the objective of this study was to identify the repertoire of proteins related to chemoreception in L. botrana by antennal transcriptome and analyze the relative expression of OBPs and CSPs in male and female antennae. Through next-generation sequencing of the antennal transcriptome by Ilumina HiSeq2500 we identified a total of 118 chemoreceptors, from which 61, 42 and 15 transcripts are related to ORs, ionotropic receptors (IRs) and gustatory receptors (GRs), respectively. Furthermore, RNA-Seq data revealed 35 transcripts for OBPs and 18 for chemosensory proteins (CSPs). Analysis by qRT-PCR showed 20 OBPs significantly expressed in female antennae, while 5 were more expressed in males. Similarly, most of the CSPs were significantly expressed in female than male antennae. All the olfactory-related sequences were compared with homologs and their phylogenetic relationships elucidated. Finally, our findings in relation to the improvement of L. botrana management are discussed.

Artículo original

The authors would like to thank Dr. Ricardo Ceballos from Instituto de Investigaciones Agropecuarias (INIA), Quilamapu, Laboratorio de Ecologia Quimica, Chillan, the financial support of INIA's Project 502185-70 and INIA La Cruz for the insects supply. This study was supported by Fondecyt de Iniciacion 11140668 and DIUFRO DFP16-0020.

Los autores agradecen al Dr. Ricardo Ceballos del Instituto de Investigaciones Agropecuarias (INIA), Quilamapu, Laboratorio de Ecología Química, Chillán, el apoyo financiero del Proyecto 502185-70 del INIA y al INIA La Cruz por el suministro de insectos. Este estudio fue apoyado por Fondecyt de Iniciacion 11140668 y DIUFRO DFP16-0020.

10.1016/j.cbd.2018.04.003

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Lobesia botrana# Chemosensory receptors# Soluble proteins# Relative expression# Antennae

http://dx.doi.org/10.1016/j.cbd.2018.04.003

Biochemistry & Molecular Biology# Genetics & Heredity

Lobesia botrana# Receptores quimiosensoriales# Proteínas solubles# Expresión relativa# Antenas

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Zoología, Ornitología, Entomología y Comportamiento Biológico

Biología Molecular

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WOS:000442710500001

Analysis of the grapevine moth <i>Lobesia botrana</i> antennal transcriptome and it expression of odorant-binding and chemosensory proteins

Artículo original

ELSEVIER SCIENCE INC

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS

Bioquímica y Biología Molecular# Genética y herencia

1744-117X

en

INIA 502185-70#UFRO DFP16-0020#ANID FONDECYT 11140668

INIA 502185-70

ANID FONDECYT 11140668

UFRO DFP16-0020

The authors would like to thank Dr. Ricardo Ceballos from Instituto de Investigaciones Agropecuarias (INIA), Quilamapu, Laboratorio de Ecologia Quimica, Chillan, the financial support of INIA's Project 502185-70 and INIA La Cruz for the insects supply. This study was supported by Fondecyt de Iniciacion 11140668 and DIUFRO DFP16-0020.

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