A catechol oxidase AcPPO from cherimoya (Annona cherimola Mill.) is localized to the Golgi apparatus

Primer Autor
Campos-Vargas, Reinaldo
Co-autores
Olmedo, Patricio#Moreno, Adrian A.#Sanhueza, Dayan#Balic, Ivan#Silva-Sanzana, Christian#Zepeda, Baltasar#Verdonk, Julian C.#Arriagada, Cesar#Meneses, Claudio
Título
A catechol oxidase AcPPO from cherimoya (Annona cherimola Mill.) is localized to the Golgi apparatus
Editorial
ELSEVIER IRELAND LTD
Revista
PLANT SCIENCE
Lenguaje
en
Resumen
Cherimoya (Annona cherimola) is an exotic fruit with attractive organoleptic characteristics. However, it is highly perishable and susceptible to postharvest browning. In fresh fruit, browning is primarily caused by the poly phenol oxidase (PPO) enzyme catalyzing the oxidation of o-diphenols to quinones, which polymerize to form brown melanin pigment. There is no consensus in the literature regarding a specific role of PPO, and its sub cellular localization in different plant species is mainly described within plastids. The present work determined the subcellular localization of a PPO protein from cherimoya (AcPPO). The obtained results revealed that the AcPPO-green fluorescent protein co-localized with a Golgi apparatus marker, and AcPPO activity was present in Golgi apparatus-enriched fractions. Likewise, transient expression assays revealed that AcPPO remained active in Golgi apparatus-enriched fractions obtained from tobacco leaves. These results suggest a putative function of AcPPO in the Golgi apparatus of cherimoya, providing new perspectives on PPO functionality in the secretory pathway, its effects on cherimoya physiology, and the evolution of this enzyme.
Tipo de Recurso
Artículo original
doi
10.1016/j.plantsci.2017.10.012
Formato Recurso
pdf
Palabras Claves
Annonaceae# Polyphenol oxidase# Secretory pathway
Annonaceae# Polyphenol oxidase# Secretory pathway
Ubicación del archivo
http://dx.doi.org/10.1016/j.plantsci.2017.10.012
Categoría OCDE
Biochemistry & Molecular Biology# Plant Sciences
Materias
Annonáceas# Polifenol oxidasa# Vía secretora
Annonáceas# Polifenol oxidasa# Vía secretora
Id de Web of Science
WOS:000423003300006
Título de la cita (Recomendado-único)
A catechol oxidase AcPPO from cherimoya (<i>Annona cherimola</i> Mill.) is localized to the Golgi apparatus
Identificador del recurso (Mandatado-único)
Artículo original
Versión del recurso (Recomendado-único)
version publicada
Editorial
ELSEVIER IRELAND LTD
Revista/Libro
PLANT SCIENCE
Categoría WOS
Bioquímica y Biología Molecular# Ciencias de las plantas
ISSN
0168-9452
Idioma
en
Formato
pdf
Tipo de ruta
hibrida#verde
Access Rights
metadata
Derechos de acceso
metadata
Página de inicio (Recomendado-único)
4173
Página final (Recomendado-único)
4188
Colecciones
Colección Publicaciones Científicas
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