Cryopreservation induces mitochondrial permeability transition in a bovine sperm model

Primer Autor
Felmer, Ricardo
Co-autores
Treulen, Favian#Arias, Maria Elena#Aguila, Luis#Uribe, Pamela
Título
Cryopreservation induces mitochondrial permeability transition in a bovine sperm model
Editorial
ACADEMIC PRESS INC ELSEVIER SCIENCE
Revista
CRYOBIOLOGY
Lenguaje
en
Resumen
When the mitochondria of somatic cells are exposed to pathological calcium overload, these trigger mitochondrial permeability transition (MPT) leading to mitochondrial dysfunction and cell death. Cryopreservation procedures expose mammalian spermatozoa to physical and chemical stressors, which affect plasma membrane integrity and induce a pathological calcium overload that gradually promotes loss of sperm quality and ultimately function. Although several studies highlight the role of calcium in many physiological and pathological processes, the MPT induced by an intracellular calcium increase and its effect on the cell quality of mammalian spermatozoa are unknown. The aim of this study was to evaluate the effects of cryopreservation on MPT and its relationship with the deterioration of sperm quality in a bovine model. To do this, frozen bovine spermatozoa were thawed and adjusted to 2 x 10(6)mL(-1) and incubated for 4hat 38 degrees C. Using flow cytometry, we evaluated MPT by the calcein-AM and cobalt chloride method, intracellular Ca2+ level using FLU03-AM, plasma membrane integrity by exclusion of propidium iodide, mitochondrial membrane potential (Delta Omega m) with tetramethylrhodamine methyl ester perchlorate and intracellular ROS production with dihydroethidium. ATP levels were assessed by a chemiluminiscent method. The results showed that thawed spermatozoa trigger MPT associated with an intracellular calcium increase and that this was accompanied by Delta Omega m dissipation, decrease of ATP levels and ROS production, and deterioration of plasma membrane integrity. In conclusion, cryopreservation induces MPT and this is associated with a loss of sperm quality.
Tipo de Recurso
Artículo original
Description
This work was supported by a Postdoctoral fellowship FONDECYT 3160214 CONICYT-Chile, and FONDECYT 1160467, CONICYT-Chile.
Este trabajo fue financiado por una beca postdoctoral FONDECYT 3160214 CONICYT-Chile, y FONDECYT 1160467, CONICYT-Chile.
doi
10.1016/j.cryobiol.2018.06.001
Formato Recurso
pdf
Palabras Claves
Bovine# Calcium# Cryopreservation# Mitochondria# Sperm
Ubicación del archivo
http://dx.doi.org/10.1016/j.cryobiol.2018.06.001
Categoría OCDE
Biology# Physiology
Materias
Bovino# Calcio# criopreservación# mitocondrias# Esperma
Disciplinas de la OCDE
Biología Reproductiva
Ciencia Animal y lechería
Biología Celular
Id de Web of Science
WOS:000441368600010
Título de la cita (Recomendado-único)
Cryopreservation induces mitochondrial permeability transition in a bovine sperm model
Identificador del recurso (Mandatado-único)
Artículo original
Versión del recurso (Recomendado-único)
version publicada
Editorial
ACADEMIC PRESS INC ELSEVIER SCIENCE
Revista/Libro
CRYOBIOLOGY
Categoría WOS
Biología# Fisiología
ISSN
0011-2240
Idioma
en
Referencia del Financiador (Mandatado si es aplicable-repetible)
ANID FONDECYT 3160214#ANID FONDECYT 1160467
ANID CONICYT FONDECYT 3160214
ANID FONDECYT 1160467
Descripción
This work was supported by a Postdoctoral fellowship FONDECYT 3160214 CONICYT-Chile, and FONDECYT 1160467, CONICYT-Chile.
Formato
pdf
Tipo de ruta
hibrida#verde
Access Rights
metadata
Derechos de acceso
metadata
Página de inicio (Recomendado-único)
121
Página final (Recomendado-único)
132
Colecciones
Colección Publicaciones Científicas
Revisa las metricas alternativas de Almetrics
Revisa las citaciones de Dimensions