Effect of incubation temperature after devitrification on quality parameters in human sperm cells

Primer Autor
Sanchez, Raul
Co-autores
Uribe, Pamela#Rojas, Christian#Merino, Juan#Zambrano, Fabiola#Villegas, Juana V.#Treulen, Favian#Boguen, Rodrigo#Isachenko, Vladimir#Isachenko, Evgenia
Título
Effect of incubation temperature after devitrification on quality parameters in human sperm cells
Editorial
ACADEMIC PRESS INC ELSEVIER SCIENCE
Revista
CRYOBIOLOGY
Lenguaje
en
Resumen
Sperm cryopreservation is common in assisted reproduction laboratories, providing a therapeutic option for several clinical conditions. This process has been optimized, however, the effect of post-thaw incubation temperature has been poorly studied. This work analyzed the effect of incubation temperature after devitrification on human sperm quality. Spermatozoa from normozoospermic donors were cryopreserved by vitrification. After devitrification, the spermatozoa were separated into two aliquots: (i) incubated at room temperature (RT, 22-25 degrees C) and (ii) incubated at 37 degrees C. Reactive oxygen species (ROS), viability, mitochondrial membrane potential (AWM), phosphatidylserine externalization and motility were analyzed immediately after devitrification (control) and after 2, 4 and 6 h. Spermatozoa incubated at RT showed a conserved viability and Delta Psi M compared to the control, while the incubation at 37 degrees C promoted a decrease in these parameters. The ROS levels were increased at both incubation conditions. The progressive motility was decreased in all experimental groups and the decrease was more pronounced under incubation at RT. No increase in phosphatidylserine externalization was observed. In conclusion, prior to use in assisted reproduction procedures, devitrified spermatozoa at RT conserve a better viability and Delta Psi M than at 37 degrees C.
Tipo de Recurso
Artículo original
Description
This work was supported by CONICYT, Chile, PAI/Concurso Nacional Insercion en la Academia, Convocatoria 2016 [Grant number PAI79160030 to P.U.], and the Direccion de Investigacion, Universidad de La Frontera, Programa de apoyo a profesores patrocinantes de alumnos de pre y postgrado [2016, to R.S].
Este trabajo fue apoyado por CONICYT, Chile, PAI/Concurso Nacional Inserción en la Academia, Convocatoria 2016 [Subvención número PAI79160030 a P.U.], y la Dirección de Investigación, Universidad de La Frontera, Programa de apoyo a profesores patrocinantes de alumnos de pre y postgrado [2016, a R.S].
doi
10.1016/j.cryobiol.2017.10.005
Formato Recurso
pdf
Palabras Claves
Sperm cryopreservation# Vitrification# Human sperm# Reactive oxygen species
Ubicación del archivo
http://dx.doi.org/10.1016/j.cryobiol.2017.10.005
Categoría OCDE
Biology# Physiology
Materias
Criopreservación de esperma# vitrificación# Esperma humano# Especies reactivas de oxígeno.
Disciplinas de la OCDE
Biología Reproductiva
Biotecnología Relacionada con la Salud
Tecnologías que involucran la manipulación de Células , Tejidos, órganos o el organismo completo (Reproducción Asistida)
Id de Web of Science
WOS:000418107400012
Título de la cita (Recomendado-único)
Effect of incubation temperature after devitrification on quality parameters in human sperm cells
Identificador del recurso (Mandatado-único)
Artículo original
Versión del recurso (Recomendado-único)
version publicada
Editorial
ACADEMIC PRESS INC ELSEVIER SCIENCE
Revista/Libro
CRYOBIOLOGY
Categoría WOS
Biología# Fisiología
ISSN
0011-2240
Idioma
en
Referencia del Financiador (Mandatado si es aplicable-repetible)
ANID CONICYT PAI79160030
UFRO DI
Descripción
This work was supported by CONICYT, Chile, PAI/Concurso Nacional Insercion en la Academia, Convocatoria 2016 [Grant number PAI79160030 to P.U.], and the Direccion de Investigacion, Universidad de La Frontera, Programa de apoyo a profesores patrocinantes de alumnos de pre y postgrado [2016, to R.S].
Formato
pdf
Tipo de ruta
hibrida#verde
Access Rights
metadata
Derechos de acceso
metadata
Página de inicio (Recomendado-único)
2288
Página final (Recomendado-único)
2297
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