Repositorio UFRO · Omeka S

Changes in sperm function and structure after freezing in domestic cat spermatozoa

Primer Autor	Risopatron, Jennie
Co-autores	Cheuqueman, Carolina#Faundez, Ricardo#Sanchez, Raul
Título	Changes in sperm function and structure after freezing in domestic cat spermatozoa
Editorial	WILEY
Revista	ANDROLOGIA
Lenguaje	en
Resumen	Sperm cryopreservation allows for a long-term storage of genetic. However, changes due to factors as cold shock, osmotic and oxidative stress cause reduction in viability and fertilising ability of frozen/thawed spermatozoa. Therefore, evaluation of cryoinjury of cat spermatozoa is a key factor in achieving better cryopreservation results. This study analysed the changes in structural and functional after freezing in ejaculated domestic cats spermatozoa. Semen samples (n=60) were analysed before and after freezing, progressive motility was determined with computer-assisted sperm analysis and viability, and acrosome intact spermatozoa, mitochondrial function and superoxide anion (O-2(-)) were assessed by flow cytometry. The results demonstrated that cryopreservation induced changes in all sperm parameters (p<0.05). Total sperm motility, viability, acrosome integrity and mitochondrial function of fresh samples were near to 80% and decrease near to 40% in frozen/thawed spermatozoa (p<0.05), nevertheless, in contrast to all other sperm parameters, the sperm positive with O-2(-) increased post/thawing (p<0.05). In conclusion, changes in frozen/thawed spermatozoa could be related to the effect of oxidative stress due to the increase in the synthesis of O-2(-) and a concomitant loss of functional competence. Therefore, the evaluation of these sperm parameters could contribute to complement the analysis of fresh or frozen semen used ART.
Tipo de Recurso	Artículo original
Description	FONDECYT POSTDOCTORADO, Grant/ Award Number: 3150320 FONDECYT POSTDOCTORADO, Número de subvención: 3150320
doi	10.1111/and.13080
Formato Recurso	pdf
Palabras Claves	domestic cat# frozen/thawed spermatozoa# sperm parameter
Ubicación del archivo	http://dx.doi.org/10.1111/and.13080
Categoría OCDE	Andrology
Materias	gato doméstico# espermatozoides congelados/descongelados# parámetro de esperma
Disciplinas de la OCDE	Biología Reproductiva Ciencias Veterinarias Animales domésticos
Versión del recurso (Recomendado-único)	version publicada
Tipo de ruta	hibrida
Access Rights	metadata
Derechos de acceso	metadata
Id de Web of Science	WOS:000446978500006
Título de la cita (Recomendado-único)	Changes in sperm function and structure after freezing in domestic cat spermatozoa
Identificador del recurso (Mandatado-único)	Artículo original
Editorial	WILEY
Revista/Libro	ANDROLOGIA
Categoría WOS	Andrología
ISSN	0303-4569
Idioma	en
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID FONDECYT 3150320 ANID FONDECYT POSTDOCTORADO 3150320
Descripción	FONDECYT POSTDOCTORADO, Grant/ Award Number: 3150320
Formato	pdf
Página de inicio (Recomendado-único)	63
Página final (Recomendado-único)	75