Changes in sperm function and structure after freezing in domestic cat spermatozoa

Primer Autor
Risopatron, Jennie
Co-autores
Cheuqueman, Carolina#Faundez, Ricardo#Sanchez, Raul
Título
Changes in sperm function and structure after freezing in domestic cat spermatozoa
Editorial
WILEY
Revista
ANDROLOGIA
Lenguaje
en
Resumen
Sperm cryopreservation allows for a long-term storage of genetic. However, changes due to factors as cold shock, osmotic and oxidative stress cause reduction in viability and fertilising ability of frozen/thawed spermatozoa. Therefore, evaluation of cryoinjury of cat spermatozoa is a key factor in achieving better cryopreservation results. This study analysed the changes in structural and functional after freezing in ejaculated domestic cats spermatozoa. Semen samples (n=60) were analysed before and after freezing, progressive motility was determined with computer-assisted sperm analysis and viability, and acrosome intact spermatozoa, mitochondrial function and superoxide anion (O-2(-)) were assessed by flow cytometry. The results demonstrated that cryopreservation induced changes in all sperm parameters (p<0.05). Total sperm motility, viability, acrosome integrity and mitochondrial function of fresh samples were near to 80% and decrease near to 40% in frozen/thawed spermatozoa (p<0.05), nevertheless, in contrast to all other sperm parameters, the sperm positive with O-2(-) increased post/thawing (p<0.05). In conclusion, changes in frozen/thawed spermatozoa could be related to the effect of oxidative stress due to the increase in the synthesis of O-2(-) and a concomitant loss of functional competence. Therefore, the evaluation of these sperm parameters could contribute to complement the analysis of fresh or frozen semen used ART.
Tipo de Recurso
Artículo original
Description
FONDECYT POSTDOCTORADO, Grant/ Award Number: 3150320
FONDECYT POSTDOCTORADO, Número de subvención: 3150320
doi
10.1111/and.13080
Formato Recurso
pdf
Palabras Claves
domestic cat# frozen/thawed spermatozoa# sperm parameter
Ubicación del archivo
http://dx.doi.org/10.1111/and.13080
Categoría OCDE
Andrology
Materias
gato doméstico# espermatozoides congelados/descongelados# parámetro de esperma
Disciplinas de la OCDE
Biología Reproductiva
Ciencias Veterinarias
Animales domésticos
Id de Web of Science
WOS:000446978500006
Título de la cita (Recomendado-único)
Changes in sperm function and structure after freezing in domestic cat spermatozoa
Identificador del recurso (Mandatado-único)
Artículo original
Versión del recurso (Recomendado-único)
version publicada
Editorial
WILEY
Revista/Libro
ANDROLOGIA
Categoría WOS
Andrología
ISSN
0303-4569
Idioma
en
Referencia del Financiador (Mandatado si es aplicable-repetible)
ANID FONDECYT 3150320
ANID FONDECYT POSTDOCTORADO 3150320
Descripción
FONDECYT POSTDOCTORADO, Grant/ Award Number: 3150320
Formato
pdf
Tipo de ruta
hibrida
Access Rights
metadata
Derechos de acceso
metadata
Página de inicio (Recomendado-único)
63
Página final (Recomendado-único)
75
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