Gradient sperm selection for reproductive techniques in cattle: Is Isolate a suitable replacement for Percoll?

Primer Autor
Felmer, R.
Co-autores
Sepulveda, B.#Arias, M. E.#Aguila, L.#Zambrano, F.#Sanchez, R.
Título
Gradient sperm selection for reproductive techniques in cattle: Is Isolate a suitable replacement for Percoll?
Editorial
WILEY
Revista
ANDROLOGIA
Lenguaje
en
Resumen
In assisted reproductive techniques, it is essential to perform a sperm selection to obtain spermatozoa with high motility and membrane integrity for in vitro fertilisation (IVF) and high-DNA integrity for intracytoplasmic sperm injection (ICSI). In this study, we evaluated whether Isolate((R)) was a suitable substitute for Percoll((R)) for assisted reproductive techniques. Commercial cryopreserved bovine semen was used after selection in both gradients, and plasma and acrosome membrane integrity, reactive oxygen species (ROS) levels, DNA integrity and mitochondrial membrane potential (m) were assessed by flow cytometry. Motility parameters were also evaluated by CASA system. A similar percentage of spermatozoa with intact plasma membrane, acrosome integrity and high m was observed in both sperm selection methods, but only Percoll((R)) showed higher percentage of spermatozoa with intact plasma and acrosome membrane compared to the post-thawing group. No differences were observed in the motility, ROS, DNA fragmentation and on the in vitro embryo production in all experimental groups. In conclusion, the selection of bovine spermatozoa with Isolate((R)) generates spermatozoa with similar quality parameters and embryonic development compared to Percoll((R)) providing a suitable alternative sperm selection method for assisted reproductive techniques in this species.
Tipo de Recurso
Artículo original
Description
Comision Nacional de Investigacion Cientifica y Tecnologica, Grant/Award Number: Proyecto PAI/No 79130018
Comisión Nacional de Investigación Científica y Tecnológica, Número de subvención/premio: Proyecto PAI/No 79130018
doi
10.1111/and.12921
Formato Recurso
pdf
Palabras Claves
bovine spermatozoa# Isolate# percoll# sperm selection
Ubicación del archivo
http://dx.doi.org/10.1111/and.12921
Categoría OCDE
Andrology
Materias
espermatozoides bovinos# Aislar# percol# selección de esperma
Disciplinas de la OCDE
Ciencia Animal y lechería
Biología Reproductiva
Biotecnología Relacionada con la Salud
Id de Web of Science
WOS:000426490200027
Título de la cita (Recomendado-único)
Gradient sperm selection for reproductive techniques in cattle: Is Isolate a suitable replacement for Percoll?
Identificador del recurso (Mandatado-único)
Artículo original
Versión del recurso (Recomendado-único)
version publicada
Editorial
WILEY
Revista/Libro
ANDROLOGIA
Categoría WOS
Andrología
ISSN
0303-4569
Idioma
en
Referencia del Financiador (Mandatado si es aplicable-repetible)
ANID CONICYT 79130018
CNPq 79130018
Descripción
Comision Nacional de Investigacion Cientifica y Tecnologica, Grant/Award Number: Proyecto PAI/No 79130018
Formato
pdf
Tipo de ruta
hibrida
Access Rights
metadata
Derechos de acceso
metadata
Colecciones
Colección Publicaciones Científicas
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