Repositorio UFRO · Omeka S

Gradient sperm selection for reproductive techniques in cattle: Is Isolate a suitable replacement for Percoll?

Primer Autor	Felmer, R.
Co-autores	Sepulveda, B.#Arias, M. E.#Aguila, L.#Zambrano, F.#Sanchez, R.
Título	Gradient sperm selection for reproductive techniques in cattle: Is Isolate a suitable replacement for Percoll?
Editorial	WILEY
Revista	ANDROLOGIA
Lenguaje	en
Resumen	In assisted reproductive techniques, it is essential to perform a sperm selection to obtain spermatozoa with high motility and membrane integrity for in vitro fertilisation (IVF) and high-DNA integrity for intracytoplasmic sperm injection (ICSI). In this study, we evaluated whether Isolate((R)) was a suitable substitute for Percoll((R)) for assisted reproductive techniques. Commercial cryopreserved bovine semen was used after selection in both gradients, and plasma and acrosome membrane integrity, reactive oxygen species (ROS) levels, DNA integrity and mitochondrial membrane potential (m) were assessed by flow cytometry. Motility parameters were also evaluated by CASA system. A similar percentage of spermatozoa with intact plasma membrane, acrosome integrity and high m was observed in both sperm selection methods, but only Percoll((R)) showed higher percentage of spermatozoa with intact plasma and acrosome membrane compared to the post-thawing group. No differences were observed in the motility, ROS, DNA fragmentation and on the in vitro embryo production in all experimental groups. In conclusion, the selection of bovine spermatozoa with Isolate((R)) generates spermatozoa with similar quality parameters and embryonic development compared to Percoll((R)) providing a suitable alternative sperm selection method for assisted reproductive techniques in this species.
Tipo de Recurso	Artículo original
Description	Comision Nacional de Investigacion Cientifica y Tecnologica, Grant/Award Number: Proyecto PAI/No 79130018 Comisión Nacional de Investigación Científica y Tecnológica, Número de subvención/premio: Proyecto PAI/No 79130018
doi	10.1111/and.12921
Formato Recurso	pdf
Palabras Claves	bovine spermatozoa# Isolate# percoll# sperm selection
Ubicación del archivo	http://dx.doi.org/10.1111/and.12921
Categoría OCDE	Andrology
Materias	espermatozoides bovinos# Aislar# percol# selección de esperma
Disciplinas de la OCDE	Ciencia Animal y lechería Biología Reproductiva Biotecnología Relacionada con la Salud
Id de Web of Science	WOS:000426490200027
Título de la cita (Recomendado-único)	Gradient sperm selection for reproductive techniques in cattle: Is Isolate a suitable replacement for Percoll?
Identificador del recurso (Mandatado-único)	Artículo original
Versión del recurso (Recomendado-único)	version publicada
Editorial	WILEY
Revista/Libro	ANDROLOGIA
Categoría WOS	Andrología
ISSN	0303-4569
Idioma	en
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID CONICYT 79130018 CNPq 79130018
Descripción	Comision Nacional de Investigacion Cientifica y Tecnologica, Grant/Award Number: Proyecto PAI/No 79130018
Formato	pdf
Tipo de ruta	hibrida
Access Rights	metadata
Derechos de acceso	metadata