Repositorio
Institucional

Farias, Jorge

Dumorne, Kelly#Valdebenito, Ivan#Risopatron, Jennie#Figueroa, Elias#Diaz, Rommy

Morphology and ultrastructure of pink cusk-eel (Genypterus blacodes, Schneider 1801) spermatozoa by scanning and transmission electron microscopy

CHURCHILL LIVINGSTONE

TISSUE & CELL

en

In this study, the morphology and ultrastructure of Genypterus blacodes spermatozoa were characterized through scanning and transmission electron microscopy. Findings revealed that the G. blacodes spermatozoa can be differentiated into three major parts: a spherical head without an acrosome (typical for externally fertilizing fish), a short mid-piece, and a long flagellum. The mean length of the spermatozoa was 57.6 +/- 6.08 mu m, with flagella accounting for 56.2 +/- 7.2 mu m. The head was 1.47 +/- 0.2 mu m long, and 0.89 +/- 0.06 mu m wide. The midpiece had a total dimension of 0.72 +/- 0.16 mu m, and was 0.31 +/- 0.02 mu m in length and 0.6 +/- 0.05 mu m in width. It was located lateral to the nucleus and contained 4 or 5 spherical mitochondria. The mitochondria were separated from the axoneme by a cytoplasmic canal. The main piece of the flagellum had short irregular sidefins, and the axoneme was composed of the typical 9+2 microtubular doublet structure enclosed by a cell membrane. The present study reveals that G. blacodes sperm can be categorized as a primitive type. This study is the first to provide comprehensive details on the morphology and ultrastructure of spermatozoa in G. blacodes.

Artículo original

This work was supported by the Fund for the Promotion of Scientific and Technological Development of FONDECYT/REGULAR No 1180387 (Dr. Jorge Farias) Universidad de la Frontera. We are grateful to the staff of the Laboratory of Electron Microscopy of University Austral (Chile), especially Dr. Orlando Garrido for their kind SEM and TEM assistance.

Este trabajo fue financiado por el Fondo para la Promoción del Desarrollo Científico y Tecnológico FONDECYT/REGULAR N.° 1180387 (Dr. Jorge Farías) de la Universidad de la Frontera. Agradecemos al personal del Laboratorio de Microscopía Electrónica de la Universidad Austral (Chile), especialmente al Dr. Orlando Garrido, por su amable apoyo en el SEM y el TEM.

10.1016/j.tice.2018.07.005

pdf

Morphology# Ultrastructure# Genypterus blacodes, spermatozoa# Scanning electron microscopy# Transmission electron microscopy

http://dx.doi.org/10.1016/j.tice.2018.07.005

Anatomy & Morphology# Cell Biology

Morfología# Ultraestructura# Genypterus blacodes, espermatozoides# Microscopía electrónica de barrido# Microscopía electrónica de transmisión.

Biología Reproductiva

Zoología, Ornitología, Entomología y Comportamiento Biológico

Biología Celular

WOS:000446691200004

Morphology and ultrastructure of pink cusk-eel (<i>Genypterus blacodes</i>, Schneider 1801) spermatozoa by scanning and transmission electron microscopy

Artículo original

version publicada

CHURCHILL LIVINGSTONE

TISSUE & CELL

Anatomía y Morfología# Biología celular

0040-8166

en

ANID FONDECYT 1180387#UACH

ANID FONDECYT REGULAR 1180387

This work was supported by the Fund for the Promotion of Scientific and Technological Development of FONDECYT/REGULAR No 1180387 (Dr. Jorge Farias) Universidad de la Frontera. We are grateful to the staff of the Laboratory of Electron Microscopy of University Austral (Chile), especially Dr. Orlando Garrido for their kind SEM and TEM assistance.

pdf

dorada#verde

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