Effect of Sperm Selection Techniques in Frozen/Thawed Cat Spermatozoa on Sperm Motility Analyzed by CASA System

Primer Autor
Risopatron, J.
Co-autores
Cheuqueman, C.#Sanchez, R.
Título
Effect of Sperm Selection Techniques in Frozen/Thawed Cat Spermatozoa on Sperm Motility Analyzed by CASA System
Editorial
SOC CHILENA ANATOMIA
Revista
INTERNATIONAL JOURNAL OF MORPHOLOGY
Lenguaje
en
Resumen
Freeze/thawing process reduces sperm survival and fertilizing ability of cat spermatozoa, with sperm motility being the most sensitive sperm parameter altered, due to cryo-damage. In this context, swim-up and density gradient processing methods can help to recover high motile and normal spermatozoa. Maximizing the use of frozen semen sample is essential, especially in endangered felids or high value cats in which sample size, number of samples or access to semen collection is reduced. To our knowledge, there is no previous report describing an in depth analysis of sperm motility improvement, after sperm selection techniques in frozen cat semen. Accordingly, we evaluated the effect of percoll gradient (PG) and swim up (SU) sperm selection techniques on sperm motility parameters and sperm recovery rate in frozen/thawed spermatozoa of domestic cat. Next, we evaluated the individual effect of the cat over sperm motility after PG sperm selection of frozen/thawed spermatozoa. SU and PG improved significantly all sperm motility parameters of frozen/thawed cat spermatozoa compared to simple washing. However, PG allows better sperm recovery from the original frozen sample and works mostly homogeneously among individual cats. This new information could help to maximize the use of frozen semen in endangered felids or high value domestic cats for its subsequent application on in vitro fertilization and artificial insemination.
Tipo de Recurso
Artículo original
Description
Authors acknowledge the FONDECYT Post-doctoral Grant No 3150320 from the Chilean Government awarded to Carolina Cheuqueman.
Los autores agradecen la Beca Postdoctoral FONDECYT No 3150320 del Gobierno de Chile otorgada a Carolina Cheuqueman.
doi
10.4067/S0717-95022017000401495
Formato Recurso
pdf
Palabras Claves
Motility# Percoll gradient# Swim up# Frozen semen# Cat spermatozoa
Ubicación del archivo
http://dx.doi.org/10.4067/S0717-95022017000401495
Categoría OCDE
Anatomy & Morphology
Materias
Motilidad# gradiente de Percoll# Nadar hacia arriba# Semen congelado# Espermatozoides de gato
Disciplinas de la OCDE
Ciencias Veterinarias
Biología Reproductiva
Tecnologías que involucran la manipulación de Células , Tejidos, órganos o el organismo completo (Reproducción Asistida)
Id de Web of Science
WOS:000428048400048
Título de la cita (Recomendado-único)
Effect of Sperm Selection Techniques in Frozen/Thawed Cat Spermatozoa on Sperm Motility Analyzed by CASA System
Identificador del recurso (Mandatado-único)
Artículo original
Versión del recurso (Recomendado-único)
version publicada
Editorial
SOC CHILENA ANATOMIA
Revista/Libro
INTERNATIONAL JOURNAL OF MORPHOLOGY
Categoría WOS
Anatomía y Morfología
ISSN
0717-9502
Idioma
en
Referencia del Financiador (Mandatado si es aplicable-repetible)
ANID FONDECYT 3150320
Descripción
Authors acknowledge the FONDECYT Post-doctoral Grant No 3150320 from the Chilean Government awarded to Carolina Cheuqueman.
Formato
pdf
Tipo de ruta
dorada#verde
Access Rights
acceso abierto
Derechos de acceso
acceso abierto
License
CC BY-NC
Colecciones
Colección Publicaciones Científicas
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