Repositorio
Institucional

Mandarim-de-Lacerda, Carlos Alberto

Del Sol, Mariano

Tips for Studies with Quantitative Morphology (Morphometry and Stereology)

SOC CHILENA ANATOMIA

INTERNATIONAL JOURNAL OF MORPHOLOGY

en

The quantitative morphology contributes to making the studies less subjective and reproducible. Quantitative results are analyzed with statistics and should be based on a well-defined sample. Quantitative variables can be 'continuous' or 'discrete.' In this text, the term 'morphometry' is used to design a simpler procedure for measuring structures with a 'ruler.' The term 'stereology' is used in estimating quantities in the 3-dimensional space analyzing 2-D cut sections. Correction factors may be necessary for the retraction and compression of the tissues. In histological sections, the 'caps' of the objects that have been sectioned tangentially are lost when chemical agents remove the paraffin distorting the analysis. Moreover, the analyses based on digital images should consider the same format and the same size (pixels) to all pictures allowing a comparison between groups. Stereology can be 'model-based': points within a frame are counted to estimate the 'volume density' (Vv) of a structure, and intercepts are counted to assess the 'surface density' (Sv). Counting structures within a frame allow estimating the 'length density' (Lv). Newer and more complex 'design-based' procedures are considered unbiased. The key point is that design-based inference does not require assumptions about the material and uses the 'random sampling' approach. The estimation of the number of objects requires a 3-D (volume) probe and therefore the 'disector' technique. This review aimed to contribute to the execution of the project, the correct sampling and the data obtained with morphometry and stereology.

Artículo original

The Laboratory in UERJ is supported by CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, grant number 302.920/2016-1) and FAPERJ (Fundacao Carlos Chagas Filho do Amparo a Pesquisa do Rio de Janeiro, grant numbers E-26/201.186/2014). The association UERJ/UFRO is funded by FAPERJ (grant number E-26/010.003.093/2014).

El Laboratorio de la UERJ cuenta con el apoyo del CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, concesión número 302.920/2016-1) y de la FAPERJ (Fundaçao Carlos Chagas Filho do Amparo a Pesquisa do Rio de Janeiro, concesión número E-26/201.186/2014). La asociación UERJ/UFRO está financiada por la FAPERJ (subvención número E-26/010.003.093/2014).

10.4067/S0717-95022017000401482

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Sample size# Digital images# Statistics# Model-based stereology# Design-based stereology

http://dx.doi.org/10.4067/S0717-95022017000401482

Anatomy & Morphology

Tamaño de la muestra# Imágenes digitales# Estadística# Estereología basada en modelos# Estereología basada en el diseño

Anatomía y Morfología

Otras Ciencias Naturales

Estadísticas y Probabilidades

WOS:000428048400047

Tips for Studies with Quantitative Morphology (Morphometry and Stereology)

Artículo original

version publicada

SOC CHILENA ANATOMIA

INTERNATIONAL JOURNAL OF MORPHOLOGY

Anatomía y Morfología

0717-9502

en

CNPq 302920/2016-1#FAPERJ E-26/201.186/2014#FAPERJ E-26/010.003.093/2014

CNPq 302.920/2016-1

FAPERJ E-26/201.186/2014

FAPERJ E-26/010.003.093/2014

The Laboratory in UERJ is supported by CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, grant number 302.920/2016-1) and FAPERJ (Fundacao Carlos Chagas Filho do Amparo a Pesquisa do Rio de Janeiro, grant numbers E-26/201.186/2014). The association UERJ/UFRO is funded by FAPERJ (grant number E-26/010.003.093/2014).

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