Repositorio
Institucional

Hidalgo, M.

Consuegra, C.#Dorado, J.#Diaz-Jimenez, M.#Ortiz, I.#Pereira, B.#Sanchez, R.#Crespo, F.

Concentrations of non-permeable cryoprotectants and equilibration temperatures are key factors for stallion sperm vitrification success

ELSEVIER

ANIMAL REPRODUCTION SCIENCE

en

Vitrification is based on rapid freezing by direct exposure of sperm to liquid nitrogen (LN2). This study evaluated the effect of non-permeable CPAs and equilibration temperature on stallion sperm quality after vitrification. In Experiment 1, different concentrations of sucrose (20, 50, 100 mM, mmol/L) and bovine serum albumin (BSA 1%, 5%, 10%) were compared including different temperatures for the equilibration ( approximate to 22 degrees C or 5 degrees C). Vitrification was performed dropping 30 mu l sperm suspension directly into LN2. In Experiment 2, conventional sperm freezing using 2.2% of glycerol in 0.5 ml straws, frozen in LN2 vapours, was compared to the sucrose and BSA extenders (and its combination) producing the most desirable results. Sperm motility, plasma membrane and acrosome integrity were statistically compared between treatments. Vitrification after sperm cooling at 5 degrees C with sucrose 20 mM (S20) or BSA 1% (BSA1) resulted in the greatest values (mean +/- SEM) for most of the sperm variables assessed. With use of the combination (S20 + BSA1/5 degrees C), there were greater values (P < 0.001) than freezing with glycerol for total (55.67 +/- 2.99 vs 35.41 +/- 2.96) and progressive sperm motility (38.32 +/- 3.05 vs 14.42 +/- 1.80), plasma membrane integrity (66.61 +/- 2.69 vs 49.16 +/- 2.60), intact-acrosomes (49.19 +/- 2.60 vs 14.91 +/- 1.57) and most of the kinetics assessed, respectively. In conclusion, stallion sperm can be vitrified after cooling at 5 degrees C using a combination of 20 mM sucrose and 1% BSA based extender and this is a promising alternative compared with conventional sperm freezing using glycerol.

Artículo original

"The authors are extremely thankful to the Ministry of Defense, especially to the equine breeding services of the Spanish Army located in Avila, Spain, for providing all animals, logistics and facilities needed to develop the experiments. This research was supported by grant AGL-2013-42726-R from Mineco (""Ministerio de Economia Industria y Competitividad""). C Consuegra was supported by the Spanish fellowships FPU from MECD (""Ministerio de Educacion, Cultura y Deporte"") and ""Semillero investigadores"" from the University of Cordoba."

"Los autores agradecen profundamente al Ministerio de Defensa, especialmente al servicio de cría equina del Ejército de Tierra español, con sede en Ávila, España, por proporcionar todos los animales, la logística y las instalaciones necesarias para el desarrollo de los experimentos. Esta investigación contó con el apoyo de la subvención AGL-2013-42726-R del Mineco (Ministerio de Economía, Industria y Competitividad). C. Consuegra recibió el apoyo de las becas españolas FPU del MECD (Ministerio de Educación, Cultura y Deporte) y del Semillero de Investigadores de la Universidad de Córdoba.

The authors are extremely thankful to the Ministry of Defense, especially to the equine breeding services of the Spanish Army located in Avila, Spain, for providing all animals, logistics and facilities needed to develop the experiments. This research was supported by grant AGL-2013-42726-R from Mineco (""Ministerio de Economia Industria y Competitividad""). C Consuegra was supported by the Spanish fellowships FPU from MECD (""Ministerio de Educacion, Cultura y Deporte"") and ""Semillero investigadores"" from the University of Cordoba."

10.1016/j.anireprosci.2018.06.022

pdf

Stallion# Sperm# Vitrification# Sucrose# BSA

http://dx.doi.org/10.1016/j.anireprosci.2018.06.022

Agriculture, Dairy & Animal Science# Reproductive Biology# Veterinary Sciences

Semental# Esperma# vitrificación# sacarosa# BSA

Ciencias Veterinarias

Ciencia Animal y lechería

Biología Reproductiva

WOS:000447110900011

Concentrations of non-permeable cryoprotectants and equilibration temperatures are key factors for stallion sperm vitrification success

Artículo original

version publicada

ELSEVIER

ANIMAL REPRODUCTION SCIENCE

Agricultura, Lácteos y Ciencia Animal# Biología Reproductiva# Ciencias Veterinarias

0378-4320

en

MINECO AGL-2013-42726-R

Ministerio de Educacion, Cultura y Deporte

Universidad de Cordoba

"The authors are extremely thankful to the Ministry of Defense, especially to the equine breeding services of the Spanish Army located in Avila, Spain, for providing all animals, logistics and facilities needed to develop the experiments. This research was supported by grant AGL-2013-42726-R from Mineco (""Ministerio de Economia Industria y Competitividad""). C Consuegra was supported by the Spanish fellowships FPU from MECD (""Ministerio de Educacion, Cultura y Deporte"") and ""Semillero investigadores"" from the University of Cordoba."

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