Repositorio
Institucional

Figueroa, E.

Magnotti, C.#Farias, J. G.#Merino, O.#Valdebenito, I.#Oliveira, R. P. S.#Cerqueira, V.

Sperm characteristics of wild and captive lebranche mullet Mugil liza (Valenciennes, 1836), subjected to sperm activation in different pH and salinity conditions

ELSEVIER SCIENCE BV

ANIMAL REPRODUCTION SCIENCE

en

In this article we describe basic aspects of the sperm biology of lebranche mullet (Mugil liza) in the wild and in captivity, in particular assessing the effects of salinity (0, 10, 20, 30, 35, 40, 50 and 60 g L-1) and pH (6, 7, 8, 9 and 10) on sperm motility. Our results indicate that the highest percentage of motility was recorded with salinity 34.6 g L-1 (95 +/- 10%) and the longest motility time was obtained with a salinity of 34.8 g L-1 (189 15 s). Variations in the salinity between 30 and 35 g L-1 did not produce any significant alterations in sperm motility, however salinities of 20 and 50 g L-1 produced a significant loss of sperm motility. The highest percentage of motility was obtained at pH 8.5 (93 +/- 12%), and the longest motility period at pH 8.7 (218 13 s), while pH lower than or equal to 7 and equal to 10 both produced a significant loss in sperm motility. A positive correlation was found between pH/salinity and the motility percentage (R-2 = 0.94 and R-2 = 0.97) and motility time (R-2 = 0.86 and R-2 = 0.98). In seminal and morphometric parameters, statistically significant differences were observed in semen volume, sperm density, plasma membrane integrity and sperm morphometry between the groups studied, showing that the characteristics of the fish have a direct influence on sperm quality. The information generated in this research will be useful for developing biotechnology tools for the effective management of Mugil liza gametes.

Artículo original

The authors are sincerely grateful for support provided by CAPES No 23038.0044323/2014-54 in Brazil, FONDECYT No1151315 and CONICYT PhD Grants in Chile (EF and OM), and to the ethics committee of the Federal University of Santa Catarina for authorising the use of animals - CEUA NoPP00861.

Los autores agradecen sinceramente el apoyo brindado por CAPES No 23038.0044323/2014-54 en Brasil, FONDECYT No1151315 y Becas de Doctorado CONICYT en Chile (EF y OM), y al comité de ética de la Universidad Federal de Santa Catarina por autorizar el uso de animales - CEUA NoPP00861.

10.1016/j.anireprosci.2018.03.004

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Spermatology# Morphology# Motility# pH# Salinity

http://dx.doi.org/10.1016/j.anireprosci.2018.03.004

Agriculture, Dairy & Animal Science# Reproductive Biology# Veterinary Sciences

Espermatología# Morfología# Motilidad# pH# Salinidad

Pesca

Biología Marina y Limnología

Biología Reproductiva

WOS:000431163900020

Sperm characteristics of wild and captive lebranche mullet <i>Mugil liza</i> (Valenciennes, 1836), subjected to sperm activation in different pH and salinity conditions

Artículo original

version publicada

ELSEVIER SCIENCE BV

ANIMAL REPRODUCTION SCIENCE

Agricultura, Lácteos y Ciencia Animal# Biología Reproductiva# Ciencias Veterinarias

0378-4320

en

CAPES 23038.0044323/2014-54#ANID FONDECYT 1151315#Federal University of Santa Catarina CEUA PP00861

CAPES 23038.0044323/2014-54

ANID FONDECYT 1151315

Federal University of Santa Catarina PP00861

The authors are sincerely grateful for support provided by CAPES No 23038.0044323/2014-54 in Brazil, FONDECYT No1151315 and CONICYT PhD Grants in Chile (EF and OM), and to the ethics committee of the Federal University of Santa Catarina for authorising the use of animals - CEUA NoPP00861.

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